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Rabbit Recombinant Monoclonal CPSF73 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388), expandable thumbnail
  • OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (AB248388), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Tested
Not recommended
Expected
Rat
Expected
Not recommended
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed:30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed:30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed:30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity).

Alternative names

CPSF73, CPSF3, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF 73 kDa subunit

Recommended products

Rabbit Recombinant Monoclonal CPSF73 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7468
Purification technique
Affinity purification
Dissociation constant
1.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248388 is the carrier-free version of Anti-CPSF73 antibody [EPR7468] ab131245.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CPSF73 also known as Cleavage and Polyadenylation Specificity Factor 73 is an important component of the mRNA processing machinery. Mechanically CPSF73 functions as an endonuclease that cleaves pre-mRNA at the 3' end a necessary step for the addition of a poly(A) tail. CPSF73 has a molecular mass of approximately 73 kDa. This protein is expressed ubiquitously across various cell types reflecting its essential role in gene expression regulation.

Biological function summary

The mRNA processing involves CPSF73 as part of the larger cleavage and polyadenylation complex. This complex is responsible for recognizing specific cleavage sites within pre-mRNA and is critical for mRNA maturation. CPSF73’s activity ensures the proper maturation of messenger RNA facilitating its export from the nucleus translation into proteins and subsequent degradation. The endonuclease works closely with other components of the complex including CPSF160 CPSF100 and CFIm suggesting that its role extends beyond just cleavage to broader mRNA stability and regulation.

Pathways

CPSF73 actively participates in the mRNA processing pathway playing an integral role in post-transcriptional regulation. It also interfaces with the spliceosome machinery illustrating the crosstalk between pre-mRNA splicing and polyadenylation sites. CPSF73 is closely related to other RNA processing proteins such as CstF-64 and Symplekin which together maintain the precise regulation of gene expression. This interrelation is particularly important in pathways where timing of mRNA processing impacts cellular function and response.

Associated diseases and disorders

CPSF73's malfunction could lead to serious implications including cancer and genetic disorders. Aberrant expression or mutations in CPSF73 have been linked to various cancers as they directly affect mRNA maturation and stability which could result in unchecked cell proliferation. Furthermore its connection with proteins such as CPSF160 has been observed in cancers where dysregulation of mRNA processing pathways contribute to tumorigenesis. Disorders like these highlight the necessity of CPSF73 for maintaining cellular health and proper gene expression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388), expandable thumbnail

    Western blot - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388)

    This data was developed using Anti-CPSF73 antibody [EPR7468] ab131245, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CPSF73 antibody [EPR7468] (Anti-CPSF73 antibody [EPR7468] ab131245) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Caco-2 cell lysate at 10 µg

    Lane 5: RAW 264.7 cell lysate at 10 µg

    Lane 6: PC-12 cell lysate at 10 µg

    Lane 7: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 77 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388)

    This data was developed using Anti-CPSF73 antibody [EPR7468] ab131245, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labelling CPSF73 with Anti-CPSF73 antibody [EPR7468] ab131245 at 1/50 dilution.

  • Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388)

    This data was developed using Anti-CPSF73 antibody [EPR7468] ab131245, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with Anti-CPSF73 antibody [EPR7468] ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388), expandable thumbnail

    OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] - BSA and Azide free (ab248388)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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