Goat Polyclonal CPT1A antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CPT1A aa 600-650.
Images
Publications
Filter by
- Biomolecules 11:2021Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJean Baptiste Nyandwi,Young Shin Ko,Hana Jin,Seung Pil Yun,Sang Won Park,Hye Jung KimPubMed 34680102
- Saudi journal of biological sciences 28:1966-19772021Short-term administration of stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdullah S Shatoor,Suliman Al Humayed,Hussain M AlmohiyPubMed 33732083
- International journal of molecular sciences 20:2019Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSithandiwe E Mazibuko-Mbeje et. al.PubMed 30717198
- The Journal of clinical investigation 120:142-562009Pharmacologic inhibition of fatty acid oxidation sensitizes human leukemia cells to apoptosis induction.Applications:WBReactive species:HumanIsmael Samudio,Romain Harmancey,Michael Fiegl,Hagop Kantarjian,Marina Konopleva,Borys Korchin,Kumar Kaluarachchi,William Bornmann,Seshagiri Duvvuri,Heinrich Taegtmeyer,Michael AndreeffPubMed 20038799
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CPT1A aa 600-650. The exact immunogen used to generate this antibody is proprietary information. Database link P50416
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed:11350182, PubMed:14517221, PubMed:16651524, PubMed:9691089). Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed:29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed:38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).
Alternative names
CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L
Recommended products
Goat Polyclonal CPT1A antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CPT1A aa 600-650.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CPT1A aa 600-650. The exact immunogen used to generate this antibody is proprietary information. Database link P50416
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.
- Biological function summary
CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.
- Pathways
CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.
- Associated diseases and disorders
CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-CPT1A antibody (ab53532)
Primary incubation was 1 hour. Detected by chemiluminescence. RIPA buffer used.
All lanes: Western blot - Anti-CPT1A antibody (ab53532) at 0.1 µg/mL
All lanes: Human Liver lysate at 35 µg
Predicted band size: 88 kDa
Observed band size: 80 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com