AB53532

Anti-CPT1A antibody

Name: Anti-CPT1A antibody (ab53532) | Abcam
Brand: Abcam Limited
SKU: AB53532
Price: 535 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal CPT1A antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CPT1A aa 600-650.

View Alternative Names

CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L

1 Images

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Western blot - Anti-CPT1A antibody (AB53532)

Primary incubation was 1 hour. Detected by chemiluminescence. RIPA buffer used.

All lanes:

Western blot - Anti-CPT1A antibody (ab53532) at 0.1 µg/mL

All lanes:

Human Liver lysate at 35 µg

Predicted band size: 88 kDa

Observed band size: 80 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CPT1A aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P50416

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.

Biological function summary

CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.

Pathways

CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.

CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed : 11350182, PubMed : 14517221, PubMed : 16651524, PubMed : 9691089). Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed : 29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed : 38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).

See full target information CPT1A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 11: PubMed34680102

2021

Rosmarinic Acid Exhibits a Lipid-Lowering Effect by Modulating the Expression of Reverse Cholesterol Transporters and Lipid Metabolism in High-Fat Diet-Fed Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Baptiste Nyandwi,Young Shin Ko,Hana Jin,Seung Pil Yun,Sang Won Park,Hye Jung Kim

Saudi journal of biological sciences 28:1966-1977 PubMed33732083

2021

Short-term administration of stimulates insulin signaling, suppresses fatty acids metabolism, and increases glucose uptake and utilization in the hearts of healthy rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah S Shatoor,Suliman Al Humayed,Hussain M Almohiy

International journal of molecular sciences 20: PubMed30717198

2019

Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sithandiwe E Mazibuko-Mbeje,Phiwayinkosi V Dludla,Candice Roux,Rabia Johnson,Samira Ghoor,Elizabeth Joubert,Johan Louw,Andy R Opoku,Christo J F Muller

The Journal of clinical investigation 120:142-56 PubMed20038799

2009

Pharmacologic inhibition of fatty acid oxidation sensitizes human leukemia cells to apoptosis induction.

Applications

Species

Human

Ismael Samudio,Romain Harmancey,Michael Fiegl,Hagop Kantarjian,Marina Konopleva,Borys Korchin,Kumar Kaluarachchi,William Bornmann,Seshagiri Duvvuri,Heinrich Taegtmeyer,Michael Andreeff

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