Name: Anti-CPT1A antibody [EPR21843-71-2F]
SKU: ab234111
Rating: 4 (1 reviews)

Rabbit Recombinant Monoclonal CPT1A antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 34 publications.

Images

Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (AB234111), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information CPT1A

Function

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed:11350182, PubMed:14517221, PubMed:16651524, PubMed:9691089). Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed:29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed:38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).

Alternative names

CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L

Recommended products

Rabbit Recombinant Monoclonal CPT1A antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 34 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21843-71-2F
Purification technique: Affinity purification Protein A
Specificity: We detected weak cross-reactivity with CPT1B with the recombinant protein only. Our WB images were generated by testing unboiled samples only.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.

Biological function summary

CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.

Pathways

CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.

Associated diseases and disorders

CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.

Product promise

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11 product images

Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Lanes 1- 2: Merged signal (red and green). Green - ab234111 observed at 88 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab234111 was shown to react with CPT1A in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human CPT1A knockout HEK-293T cell line ab266319 (knockout cell lysate Human CPT1A knockout HEK-293T cell lysate ab256880) was used. Wild-type HEK-293T and CPT1A knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab234111 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: CPT1A knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human CPT1A knockout HEK-293T cell line (Human CPT1A knockout HEK-293T cell line ab266319)
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 37 seconds; Lane 4: 114 seconds; Lane 5: 15 seconds; Lanes 6 and 7: 92 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1: C6 (rat glial tumor cell line) whole cell lysate at 20 µg
Lane 2: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3: Mouse kidney lysate at 20 µg
Lane 4: Rat kidney lysate at 20 µg
Lane 5: Human kidney lysate at 20 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 7: SK-OV-3 (human ovarian cancer cell line) whole cell lysate at 20 µg
Secondary
Lanes 1, 2, 3, 4, 6 and 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 5: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in rat kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human ovarian carcinoma (PMID: 26716645) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Blocking/Dilution buffer: 5% NFDM/TBST.
ab234111 was shown to specifically react with CPT1A in wild-type HAP1 cells as signal was lost in CPT1A knockout cells. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. ab234111 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
All lanes: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: CPT1A knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: SK-OV-3 (human ovarian cancer cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 92s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Immunohistochemical analysis of paraffin-embedded human heart tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human heart is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Blocking and diluting buffer: 5% NFDM /TBST
Lanes 1 - 2: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lanes 1 - 2: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] - BSA and Azide free (Anti-CPT1A antibody [EPR21843-71-2F] - BSA and Azide free ab235841) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 1/20 dilution
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Observed band size: 88 kDa
Exposure time: 92s
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Blocking and diluting buffer: 5% NFDM/TBST
Lanes 1 - 3: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] - BSA and Azide free (Anti-CPT1A antibody [EPR21843-71-2F] - BSA and Azide free ab235841) at 1/1000 dilution
Lanes 1 - 3: Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 3: HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 88 kDa
Exposure time: 180s
Flow Cytometry (Intracellular) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SK-OV-3 (human ovarian cancer cell line) cell line labeling CPT1A with ab234111 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.