Rabbit Polyclonal CPT1B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 25 publications.
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- Nature metabolism 5:1290-13022023Fatty acid oxidation organizes mitochondrial supercomplexes to sustain astrocytic ROS and cognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrenda Morant-Ferrando,Daniel Jimenez-Blasco,Paula Alonso-Batan,Jesús Agulla,Rebeca Lapresa,Dario Garcia-Rodriguez,Sara Yunta-Sanchez,Irene Lopez-Fabuel,Emilio Fernandez,Peter Carmeliet,Angeles Almeida,Marina Garcia-Macia,Juan P BolañosPubMed 37460843
- Human molecular genetics 32:1922-19312023Nicotinamide riboside rescues dysregulated glycolysis and fatty acid β-oxidation in a human hepatic cell model of citrin deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWinifred W Yau,Gao Bin Chen,Jin Zhou,Joel Celio Francisco,Nivetha Kanakaram Thimmukonda,Shang Li,Brijesh Kumar Singh,Paul Michael YenPubMed 36881658
- Scientific reports 12:145762022PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC-1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yang Huang,Shin-Ichi Oka,Xiaoyong Xu,Chian-Feng Chen,Chien-Yi Tung,Ya-Yuan Chang,Youssef Mourad,Omair Vehra,Andreas Ivessa,Ghassan Yehia,Peter Romanienko,Chiao-Po Hsu,Junichi SadoshimaPubMed 36028747
- Frontiers in physiology 13:8592462022Disrupting AMPK-Glycogen Binding in Mice Increases Carbohydrate Utilization and Reduces Exercise Capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalie R Janzen,Jamie Whitfield,Lisa Murray-Segal,Bruce E Kemp,John A Hawley,Nolan J HoffmanPubMed 35392375
- Foods (Basel, Switzerland) 10:2021A Multi-Ingredient Formula Ameliorates Exercise-Induced Fatigue by Changing Metabolic Pathways and Increasing Antioxidant Capacity in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Chen,Xuan Ma,Lixing Cao,Shuang Zhao,Chong Zhao,Shutao Yin,Hongbo HuPubMed 34945671
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e214662021Mixed conjugated linoleic acid sex-dependently reverses high-fat diet-induced insulin resistance via the gut-adipose axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoyu Li,Pan Zhuang,Yu Zhang,Qiyang Shou,Yanhua Lu,Guangfa Wang,Jieni Qiu,Jun Wang,Lilin He,Jingnan Chen,Jingjing JiaoPubMed 33734496
- Experimental physiology 106:1208-12232021Resting skeletal muscle PNPLA2 (ATGL) and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver J Chrzanowski-Smith,Robert M Edinburgh,Eleanor Smith,Mark P Thomas,Jean-Philippe Walhin,Francoise Koumanov,Sean Williams,James A Betts,Javier T GonzalezPubMed 33675111
- Diabetes, metabolic syndrome and obesity : targets and therapy 14:1233-12412021The β3 Adrenergic Receptor Agonist CL316243 Ameliorates the Metabolic Abnormalities of High-Fat Diet-Fed Rats by Activating AMPK/PGC-1α Signaling in Skeletal Muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Na Ding,Ya Cheng,Lu-Yao Xu,Le-Quan Zhou,Li Guan,Hai-Mei Liu,Ya-Xing Zhang,Run-Mei Li,Jin-Wen XuPubMed 33776460
- American journal of physiology. Regulatory, integrative and comparative physiology 320:R547-R5622020Proteomic analysis of mitochondrial biogenesis in cardiomyocytes differentiated from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSundararajan Venkatesh,Erdene Baljinnyam,Mingming Tong,Toshihide Kashihara,Lin Yan,Tong Liu,Hong Li,Lai-Hua Xie,Michinari Nakamura,Shin-Ichi Oka,Carolyn K Suzuki,Diego Fraidenraich,Junichi SadoshimaPubMed 33112656
- Cell death discovery 6:1062020Cardiomyocyte-restricted high-mobility group box 1 (HMGB1) deletion leads to small heart and glycolipid metabolic disorder through GR/PGC-1α signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Yu et. al.PubMed 33101708
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Horse, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Horse, Dog, Pig, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.
Alternative names
KIAA1670, CPT1B, CPT1-M, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein, CPT I, CPTI-M
Recommended products
Rabbit Polyclonal CPT1B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 25 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab134988 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab134135.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Carnitine palmitoyltransferase 1B (CPT1B) also known as CPT1-M is an enzyme important for fatty acid metabolism. This protein has a molecular weight of about 88 kDa. It is expressed mainly in skeletal muscle tissue and heart. CPT1B is involved in the transport of long-chain fatty acids into the mitochondria which is necessary for beta-oxidation an important metabolic process where fatty acids are broken down to generate energy.
- Biological function summary
CPT1B serves an important role in energy metabolism by enabling the conversion of fatty acids into energy within muscle cells. This protein functions as part of the carnitine shuttle complex in conjunction with carnitine-acylcarnitine translocase (CACT) and carnitine palmitoyltransferase 2 (CPT2). This system collectively facilitates the translocation of fatty acids into the mitochondria for subsequent breakdown and energy production.
- Pathways
CPT1B plays a role in fatty acid oxidation and energy metabolism pathways. It helps regulate the balance of energy production by modulating the entry of fatty acids into mitochondria. In these pathways CPT1B interacts with CPT2 and CACT ensuring efficient fatty acid transport and oxidation. Through these interactions it helps maintain energy homeostasis especially in tissues with high metabolic demands like muscle and heart.
- Associated diseases and disorders
CPT1B has associations with metabolic conditions such as obesity and insulin resistance. When CPT1B function is impaired it can lead to inefficient fatty acid metabolism contributing to lipid accumulation and metabolic dysfunction. Also connections exist between CPT1B activity and symptoms of metabolic syndrome where alterations in CPT1B expression may impact energy utilization and fat storage in muscle tissue.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1B antibody (ab134988)
IHC image of CPT1B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134988, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-CPT1B antibody (ab134988)
Please note that Human Liver tissue lysate in Lane 4 was used as a negative control.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134988 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-CPT1B antibody (ab134988) at 1 µg/mL
Lane 1: Heart (Human) Tissue Lysate - adult normal tissue at 25 µg
Lane 2: Heart (Human) Whole Cell Lysate - fetal normal tissue at 25 µg
Lane 3: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 25 µg
Lane 4: Liver (Human) Tissue Lysate - adult normal tissue (Negative control) at 25 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 4min
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