Anti-CPT1B antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1B antibody (AB134988)

IHC image of CPT1B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134988, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

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Western blot - Anti-CPT1B antibody (AB134988)

Please note that Human Liver tissue lysate in Lane 4 was used as a negative control.

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134988 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-CPT1B antibody (ab134988) at 1 µg/mL

Lane 1:

Heart (Human) Tissue Lysate - adult normal tissue at 25 µg

Lane 2:

Heart (Human) Whole Cell Lysate - fetal normal tissue at 25 µg

Lane 3:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 25 µg

Lane 4:

Liver (Human) Tissue Lysate - adult normal tissue (Negative control) at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

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Exposure time: 4min