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AB248695

Anti-CPT1B antibody [EPR7838] - BSA and Azide free

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Rabbit Recombinant Monoclonal CPT1B antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA1670, CPT1B, CPT1-M, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein, CPT I, CPTI-M

2 Images
Western blot - Anti-CPT1B antibody [EPR7838] - BSA and Azide free (AB248695)
  • WB

Unknown

Western blot - Anti-CPT1B antibody [EPR7838] - BSA and Azide free (AB248695)

This data was developed using ab134135, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CPT1B antibody [EPR7838] (<a href='/en-us/products/primary-antibodies/cpt1b-antibody-epr7838-ab134135'>ab134135</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 87 kDa

false

OI-RD Scanning - Anti-CPT1B antibody [EPR7838] - BSA and Azide free (AB248695)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CPT1B antibody [EPR7838] - BSA and Azide free (AB248695)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7838

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248695 is the carrier-free version of ab134135.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carnitine palmitoyltransferase 1B (CPT1B) also known as CPT1-M is an enzyme important for fatty acid metabolism. This protein has a molecular weight of about 88 kDa. It is expressed mainly in skeletal muscle tissue and heart. CPT1B is involved in the transport of long-chain fatty acids into the mitochondria which is necessary for beta-oxidation an important metabolic process where fatty acids are broken down to generate energy.
Biological function summary

CPT1B serves an important role in energy metabolism by enabling the conversion of fatty acids into energy within muscle cells. This protein functions as part of the carnitine shuttle complex in conjunction with carnitine-acylcarnitine translocase (CACT) and carnitine palmitoyltransferase 2 (CPT2). This system collectively facilitates the translocation of fatty acids into the mitochondria for subsequent breakdown and energy production.

Pathways

CPT1B plays a role in fatty acid oxidation and energy metabolism pathways. It helps regulate the balance of energy production by modulating the entry of fatty acids into mitochondria. In these pathways CPT1B interacts with CPT2 and CACT ensuring efficient fatty acid transport and oxidation. Through these interactions it helps maintain energy homeostasis especially in tissues with high metabolic demands like muscle and heart.

CPT1B has associations with metabolic conditions such as obesity and insulin resistance. When CPT1B function is impaired it can lead to inefficient fatty acid metabolism contributing to lipid accumulation and metabolic dysfunction. Also connections exist between CPT1B activity and symptoms of metabolic syndrome where alterations in CPT1B expression may impact energy utilization and fat storage in muscle tissue.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.
See full target information CPT1B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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