Anti-Cpt1c/CPT1-B antibody [EPR28434-111] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] - BSA and Azide free (AB316934)

This data was developed using ab316933, the same antibody clone in a different buffer formulation.

Cpt1c/CPT1-B was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab316933 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316933 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : ab316933 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316933 in SH-SY5Y whole cell lysate

All lanes:

Immunoprecipitation - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] (<a href='/en-us/products/primary-antibodies/cpt1c-cpt1-b-antibody-epr28434-111-ab316933'>ab316933</a>) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] - BSA and Azide free (AB316934)

This data was developed using ab316933, the same antibody clone in a different buffer formulation.

Negative control : spleen (PMID : 12376098), liver (PMID : 12376098, PMID : 18192268).

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the bands at approximately 150 kDa (in lanes 1-4) and 40 kDa (in lane 5) are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] (<a href='/en-us/products/primary-antibodies/cpt1c-cpt1-b-antibody-epr28434-111-ab316933'>ab316933</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Human hypothalamus tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Human hippocampus tissue lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Human spleen tissue lysate at 20 µg with 5% NFDM/TBST

Lane 6:

Human liver tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] - BSA and Azide free (AB316934)

This data was developed using ab316933, the same antibody clone in a different buffer formulation.

Negative control : 293T (PMID : 18192268), HEL.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cpt1c/CPT1-B antibody [EPR28434-111] (<a href='/en-us/products/primary-antibodies/cpt1c-cpt1-b-antibody-epr28434-111-ab316933'>ab316933</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

HEL (erythroleukemia erythroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 103s