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AB110293

Anti-CPT2 antibody [1C2AE6]

1

(1 Review)

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(9 Publications)

Mouse Monoclonal CPT2 antibody. Suitable for IP, Flow Cyt, ICC/IF, IHC-P and reacts with Mouse, Rat, Human, Cow samples. Cited in 9 publications.

View Alternative Names

CPT1, CPT2, Carnitine palmitoyltransferase II, CPT II

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (AB110293)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (AB110293)

Immunocytochemistry image of ab110293 stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110293 at 4 µg/ml for 2h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (AB110293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (AB110293)

IHC image of CPT2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110293, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (AB110293)
  • Flow Cyt

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Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (AB110293)

HL-60 cells were stained with 1 µg/mL of ab110293 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (AB110293)
  • IP

Unknown

Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (AB110293)

SDS-PAGE analysis of IP samples. As an IP antibody, ab110293 works with human, bovine, rat, and mouse samples.

Lane STD : Molecular weight ladder

Lane HLM : Human liver mitochondria

Lane BHM : Bovine heart mitochondria

Lane RLM : Rat liver mitochondria

Lane MLM : Mouse liver mitochondria

All lanes:

Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)

Predicted band size: 74 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C2AE6

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, IHC-P, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carnitine palmitoyltransferase 2 (CPT2) also known as CPT-2 is an enzyme located mainly in the mitochondria of liver muscle and heart tissues. This enzyme has a molecular mass of approximately 68 kDa. CPT2 plays a mechanical role in the transfer of long-chain fatty acids from the cytosol into the mitochondrial matrix completing the process initiated by CPT1 on the outer mitochondrial membrane. CPT2 acts on the inner mitochondrial membrane converting acylcarnitine back into acyl-CoA which is an essential step in beta-oxidation. Expression of CPT2 is higher in tissues with elevated fatty acid oxidation rates.
Biological function summary

CPT2 is integral to the mitochondrial pathway of fatty acid metabolism. It functions as part of the carnitine shuttle system a complex important for the conversion of acylcarnitine to acyl-CoA inside the mitochondria. This role is vital for the full oxidation of long-chain fatty acids. Through its action CPT2 aids in maintaining energy homeostasis and producing ketone bodies especially during periods of fasting or strenuous exercise.

Pathways

CPT2 is involved in both the beta-oxidation and the carnitine shuttle pathways. These pathways facilitate the energy production from fatty acids particularly during states of low carbohydrate availability. CPT2 works closely with CPT1 as both enzymes form the gateway for the entry of long-chain fatty acids into mitochondria. Together with enzymes from the tricarboxylic acid cycle CPT2 helps sustain ATP production under such metabolic conditions.

Defects in CPT2 can lead to disorders like CPT2 deficiency which present with symptoms of muscle weakness and recurrent rhabdomyolysis especially during prolonged fasting or significant exercise. This deficiency can also result in hypoketotic hypoglycemia and liver dysfunction. CPT2-related disorders often involve altered interactions with proteins such as the acyl-CoA dehydrogenases impacting the normal fatty acid oxidation process and causing metabolic disturbances.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed : 20538056, PubMed : 24780397). Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters (PubMed : 20538056).
See full target information CPT2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:725-744 PubMed39781464

2025

Mitochondrial SIRT2-mediated CPT2 deacetylation prevents diabetic cardiomyopathy by impeding cardiac fatty acid oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Guo,Ziyin Zhang,Zheng Wen,Xiaonan Kang,Dan Wang,Lu Zhang,Mengke Cheng,Gang Yuan,Huihui Ren

Cell research 34:13-30 PubMed38163844

2024

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzi Mao,Jiaojiao Zhang,Qian Zhou,Xiadi He,Zhifang Zheng,Yun Wei,Kaiqiang Zhou,Yan Lin,Haowen Yu,Haihui Zhang,Yineng Zhou,Pengcheng Lin,Baixing Wu,Yiyuan Yuan,Jianyuan Zhao,Wei Xu,Shimin Zhao

Signal transduction and targeted therapy 8:229 PubMed37321990

2023

Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Boyuan Ma,Anji Ju,Shaosen Zhang,Qi An,Siran Xu,Jie Liu,Li Yu,Yan Fu,Yongzhang Luo

Frontiers in pharmacology 13:905092 PubMed35814216

2022

Notoginsenoside R1 Regulates Ischemic Myocardial Lipid Metabolism by Activating the AKT/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lei,Yiqi Yan,Yaolei Ma,Min Jiang,Boli Zhang,Han Zhang,Yuhong Li

Developmental cell 52:196-209.e9 PubMed31866205

2019

Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Liu,Bowen Xie,Ming Fan,Demet Candas-Green,Joy X Jiang,Ryan Wei,Yinsheng Wang,Hong-Wu Chen,Yiyang Hu,Jian Jian Li

Molecular nutrition & food research 62:e1800315 PubMed29883529

2018

The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyu Zhang,Tiantian Zhang,Lin Ding,Jie Xu,Changhu Xue,Teruyoshi Yanagita,Yaoguang Chang,Yuming Wang

Journal of neurochemistry 142:407-419 PubMed28512781

2017

Developmental regulation and localization of carnitine palmitoyltransferases (CPTs) in rat brain.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer N Jernberg,Caitlyn E Bowman,Michael J Wolfgang,Susanna Scafidi

The Journal of clinical investigation 126:2642-60 PubMed27294527

2016

Programmed death ligand-1 expression on donor T cells drives graft-versus-host disease lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Asim Saha,Roddy S O'Connor,Govindarajan Thangavelu,Scott B Lovitch,Durga Bhavani Dandamudi,Caleph B Wilson,Benjamin G Vincent,Victor Tkachev,Jan M Pawlicki,Scott N Furlan,Leslie S Kean,Kazutoshi Aoyama,Patricia A Taylor,Angela Panoskaltsis-Mortari,Rocio Foncea,Parvathi Ranganathan,Steven M Devine,Joel S Burrill,Lili Guo,Catarina Sacristan,Nathaniel W Snyder,Ian A Blair,Michael C Milone,Michael L Dustin,James L Riley,David A Bernlohr,William J Murphy,Brian T Fife,David H Munn,Jeffrey S Miller,Jonathan S Serody,Gordon J Freeman,Arlene H Sharpe,Laurence A Turka,Bruce R Blazar

Antioxidants & redox signaling 19:243-57 PubMed22938691

2012

Increased mitochondrial activity in BMP7-treated brown adipocytes, due to increased CPT1- and CD36-mediated fatty acid uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Kristy L Townsend,Ding An,Matthew D Lynes,Tian Lian Huang,Hongbin Zhang,Laurie J Goodyear,Yu-Hua Tseng
View all publications
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