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Mouse Monoclonal CPT2 antibody. Suitable for IP, Flow Cyt, ICC/IF, IHC-P and reacts with Mouse, Rat, Human, Cow samples. Cited in 7 publications.


Images

Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (AB110293), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (AB110293), expandable thumbnail
  • Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (AB110293), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (AB110293), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Tested
Not recommended
Tested
Tested
Mouse
Tested
Expected
Not recommended
Expected
Expected
Rat
Tested
Expected
Not recommended
Expected
Expected
Cow
Tested
Expected
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse, Rat, Human, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
4 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed:20538056, PubMed:24780397). Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters (PubMed:20538056).

Alternative names

CPT1, CPT2, Carnitine palmitoyltransferase II, CPT II

Recommended products

Mouse Monoclonal CPT2 antibody. Suitable for IP, Flow Cyt, ICC/IF, IHC-P and reacts with Mouse, Rat, Human, Cow samples. Cited in 7 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
1C2AE6
Purification technique
Precipitation Ammonium Sulphate
Light chain type
kappa
Concentration
Loading...
Purification notes

Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Carnitine palmitoyltransferase 2 (CPT2) also known as CPT-2 is an enzyme located mainly in the mitochondria of liver muscle and heart tissues. This enzyme has a molecular mass of approximately 68 kDa. CPT2 plays a mechanical role in the transfer of long-chain fatty acids from the cytosol into the mitochondrial matrix completing the process initiated by CPT1 on the outer mitochondrial membrane. CPT2 acts on the inner mitochondrial membrane converting acylcarnitine back into acyl-CoA which is an essential step in beta-oxidation. Expression of CPT2 is higher in tissues with elevated fatty acid oxidation rates.

Biological function summary

CPT2 is integral to the mitochondrial pathway of fatty acid metabolism. It functions as part of the carnitine shuttle system a complex important for the conversion of acylcarnitine to acyl-CoA inside the mitochondria. This role is vital for the full oxidation of long-chain fatty acids. Through its action CPT2 aids in maintaining energy homeostasis and producing ketone bodies especially during periods of fasting or strenuous exercise.

Pathways

CPT2 is involved in both the beta-oxidation and the carnitine shuttle pathways. These pathways facilitate the energy production from fatty acids particularly during states of low carbohydrate availability. CPT2 works closely with CPT1 as both enzymes form the gateway for the entry of long-chain fatty acids into mitochondria. Together with enzymes from the tricarboxylic acid cycle CPT2 helps sustain ATP production under such metabolic conditions.

Associated diseases and disorders

Defects in CPT2 can lead to disorders like CPT2 deficiency which present with symptoms of muscle weakness and recurrent rhabdomyolysis especially during prolonged fasting or significant exercise. This deficiency can also result in hypoketotic hypoglycemia and liver dysfunction. CPT2-related disorders often involve altered interactions with proteins such as the acyl-CoA dehydrogenases impacting the normal fatty acid oxidation process and causing metabolic disturbances.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293), expandable thumbnail

    Flow Cytometry - Anti-CPT2 antibody [1C2AE6] (ab110293)

    HL-60 cells were stained with 1 µg/mL of ab110293 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  • Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [1C2AE6] (ab110293)

    Immunocytochemistry image of ab110293 stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110293 at 4 µg/ml for 2h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

  • Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293), expandable thumbnail

    Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)

    SDS-PAGE analysis of IP samples. As an IP antibody, ab110293 works with human, bovine, rat, and mouse samples.

    Lane STD: Molecular weight ladder

    Lane HLM: Human liver mitochondria

    Lane BHM: Bovine heart mitochondria

    Lane RLM: Rat liver mitochondria

    Lane MLM: Mouse liver mitochondria

    All lanes: Immunoprecipitation - Anti-CPT2 antibody [1C2AE6] (ab110293)

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [1C2AE6] (ab110293)

    IHC image of CPT2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110293, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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