AB326710

Anti-CPT2 antibody [3B10]

Recombinant
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Rabbit Recombinant Monoclonal CPT2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

CPT1, CPT2, Carnitine palmitoyltransferase II, CPT II

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT2 antibody [3B10] (AB326710)

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CPT2 Monoclonal Antibody, Unconjugated (ab326710) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

Supplier Data

Western blot - Anti-CPT2 antibody [3B10] (AB326710)

25 ug total protein per lane of various lysates (see on figure) probed with CPT2 monoclonal antibody, unconjugated (ab326710) at 1 : 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

All lanes:

Western blot - Anti-CPT2 antibody [3B10] (ab326710) at 1/1000 dilution

Lane 1:

Mouse liver at 25 µg

Lane 2:

Mouse kidney at 25 µg

Lane 3:

Mouse heart at 25 µg

Lane 4:

Rat heart at 25 µg

Lane 5:

LNCaP at 25 µg

Lane 6:

HeLa at 25 µg

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

3B10

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 1% TBS, 1X

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|The product can be stored for up to 12 months

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed : 20538056, PubMed : 24780397). Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters (PubMed : 20538056).

See full target information CPT2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com