Rabbit Recombinant Monoclonal CRABP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--immunoprecipitation-img74001.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)")
![Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--western-blot-img54384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)")
![Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--western-blot-img123648.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
Alternative names
RBP5, CRABP1, Cellular retinoic acid-binding protein 1, Cellular retinoic acid-binding protein I, CRABP-I
Recommended products
Rabbit Recombinant Monoclonal CRABP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7905
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250626 is the carrier-free version of Anti-CRABP1 antibody [EPR7905] ab182573.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRABP1 also known as Cellular Retinoic Acid Binding Protein 1 is a small protein with a molecular mass of around 15 kDa. It mainly binds and transports retinoic acid a metabolite of vitamin A that is important for cell growth and differentiation. CRABP1 is typically expressed in various tissues including the skin eyes and central nervous system. It provides cells with a way to regulate retinoic acid levels acting as a buffer and maintaining proper cellular functioning.
- Biological function summary
CRABP1 plays a significant role in modulating the access of retinoic acid to nuclear receptors by controlling its availability in the cell. It is not part of a large protein complex but instead often acts independently to manage intracellular retinoic acid. The protein ensures normal development and maintenance of tissues by influencing gene transcription related to cell differentiation and proliferation. This regulation is important in processes like embryogenesis and skin regeneration.
- Pathways
Several intricate signaling cascades involve CRABP1 such as the retinoic acid signaling pathway and the retinoid metabolic pathway. In these pathways CRABP1 directly interacts with proteins like Retinoic Acid Receptors (RARs) to modulate their activity. By regulating retinoic acid levels CRABP1 influences cellular response outcomes which is important for processes like morphogenesis and homeostasis.
- Associated diseases and disorders
CRABP1 has been linked to specific conditions like skin disorders and certain types of cancer. Altered levels of CRABP1 can impact the normal regulation of retinoic acid leading to dysfunctional cell proliferation and differentiation. The protein's interaction with RAR-related proteins plays a role in these diseases where disrupted signaling can result in anomalous cellular behavior contributing to pathological development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--immunoprecipitation-img74001.jpg "Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)")
Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)
This data was developed using Anti-CRABP1 antibody [EPR7905] ab182573, the same antibody clone in a different buffer formulation.Western blot analysis of CRABP1 in immunoprecipitation pellets from Human Y79 lysate. Anti-CRABP1 antibody [EPR7905] ab182573 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
All lanes: Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] (Anti-CRABP1 antibody [EPR7905] ab182573)
Predicted band size: 15 kDa
![Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--western-blot-img54384.jpg "Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)")
Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)
This data was developed using Anti-CRABP1 antibody [EPR7905] ab182573, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CRABP1 antibody [EPR7905] (Anti-CRABP1 antibody [EPR7905] ab182573) at 1/1000 dilution
All lanes: Human skin lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 15 kDa
![Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626), expandable thumbnail](https://content.abcam.com/products/images/crabp1-antibody-epr7905-bsa-and-azide-free-ab250626--western-blot-img123648.jpg "Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)")
Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (ab250626)
This data was developed using Anti-CRABP1 antibody [EPR7905] ab182573, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CRABP1 antibody [EPR7905] (Anti-CRABP1 antibody [EPR7905] ab182573) at 1/1000 dilution
Lane 1: Y79 cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
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