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AB250626

Anti-CRABP1 antibody [EPR7905] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CRABP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RBP5, CRABP1, Cellular retinoic acid-binding protein 1, Cellular retinoic acid-binding protein I, CRABP-I

3 Images
Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)
  • IP

Supplier Data

Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)

This data was developed using ab182573, the same antibody clone in a different buffer formulation.

Western blot analysis of CRABP1 in immunoprecipitation pellets from Human Y79 lysate. ab182573 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

All lanes:

Immunoprecipitation - Anti-CRABP1 antibody [EPR7905] (<a href='/en-us/products/primary-antibodies/crabp1-antibody-epr7905-ab182573'>ab182573</a>)

Predicted band size: 15 kDa

false

Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)
  • WB

Supplier Data

Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)

This data was developed using ab182573, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRABP1 antibody [EPR7905] (<a href='/en-us/products/primary-antibodies/crabp1-antibody-epr7905-ab182573'>ab182573</a>) at 1/1000 dilution

Lane 1:

Y79 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)
  • WB

Supplier Data

Western blot - Anti-CRABP1 antibody [EPR7905] - BSA and Azide free (AB250626)

This data was developed using ab182573, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRABP1 antibody [EPR7905] (<a href='/en-us/products/primary-antibodies/crabp1-antibody-epr7905-ab182573'>ab182573</a>) at 1/1000 dilution

All lanes:

Human skin lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7905

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250626 is the carrier-free version of ab182573.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRABP1 also known as Cellular Retinoic Acid Binding Protein 1 is a small protein with a molecular mass of around 15 kDa. It mainly binds and transports retinoic acid a metabolite of vitamin A that is important for cell growth and differentiation. CRABP1 is typically expressed in various tissues including the skin eyes and central nervous system. It provides cells with a way to regulate retinoic acid levels acting as a buffer and maintaining proper cellular functioning.
Biological function summary

CRABP1 plays a significant role in modulating the access of retinoic acid to nuclear receptors by controlling its availability in the cell. It is not part of a large protein complex but instead often acts independently to manage intracellular retinoic acid. The protein ensures normal development and maintenance of tissues by influencing gene transcription related to cell differentiation and proliferation. This regulation is important in processes like embryogenesis and skin regeneration.

Pathways

Several intricate signaling cascades involve CRABP1 such as the retinoic acid signaling pathway and the retinoid metabolic pathway. In these pathways CRABP1 directly interacts with proteins like Retinoic Acid Receptors (RARs) to modulate their activity. By regulating retinoic acid levels CRABP1 influences cellular response outcomes which is important for processes like morphogenesis and homeostasis.

CRABP1 has been linked to specific conditions like skin disorders and certain types of cancer. Altered levels of CRABP1 can impact the normal regulation of retinoic acid leading to dysfunctional cell proliferation and differentiation. The protein's interaction with RAR-related proteins plays a role in these diseases where disrupted signaling can result in anomalous cellular behavior contributing to pathological development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
See full target information CRABP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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