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AB250468

Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal CRABP2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II, CRABP-II, CRABP2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.

Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • WB

Supplier Data

Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRABP2 antibody [EPR14256(B)] (<a href='/en-us/products/primary-antibodies/crabp2-antibody-epr14256b-ab181255'>ab181255</a>) at 1/50000 dilution

All lanes:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

true

Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)
  • WB

Supplier Data

Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (AB250468)

This data was developed using ab181255, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRABP2 antibody [EPR14256(B)] (<a href='/en-us/products/primary-antibodies/crabp2-antibody-epr14256b-ab181255'>ab181255</a>) at 1/10000 dilution

All lanes:

Human skin cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14256(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250468 is the carrier-free version of ab181255.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cellular Retinoic Acid-Binding Protein 2 (CRABP2) mechanically facilitates cellular retinoic acid (RA) signaling by transporting RA to the nucleus. The protein is also known as CRABP II and is approximately 15 kDa in mass. You can find its expression in various tissues including skin adipose tissue and brain where it exhibits specific roles in RA signaling and cellular differentiation processes.
Biological function summary

CRABP2 participates in modulating the availability of RA which influences gene expression by binding to nuclear receptors. Although CRABP2 functions independently rather than as part of a larger protein complex it holds a significant role in gene transcription regulation. This active role in regulating RA levels impacts cellular differentiation and proliferation processes essential for tissue development and homeostasis.

Pathways

CRABP2 is an important participant in the retinoic acid signaling pathway. This pathway impacts important biological processes such as embryonic development and cell growth. CRABP2 closely interacts with the retinoic acid receptor (RAR) guiding RA to RARs and contributing to the regulation of gene expression. Moreover it is linked to the transforming growth factor-beta (TGF-beta) pathway playing a role in managing cellular apoptosis and differentiation.

Researchers associate CRABP2 with several cancers notably skin cancer due to its influence on cell proliferation. Altered expression or function of CRABP2 can also affect neurodegenerative diseases impacting pathways involving neuronal survival. CRABP2's interactions with proteins like RAR and in cancer contexts with overexpressed RA receptors highlight its relevance in modulating RA-dependent processes that can lead to pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
See full target information CRABP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com