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AB154898

Anti-CRALBP antibody

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(4 Publications)

Rabbit Polyclonal CRALBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus.

View Alternative Names

CRALBP, RLBP1, Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody (AB154898)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody (AB154898)

Immunohistochemical analysis of mouse eye tissue stained for CRALBP protein with ab154898 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody (AB154898)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody (AB154898)

Immunohistochemical analysis of rat eye tissue stained for CRALBP protein with ab154898 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-CRALBP antibody (AB154898)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRALBP antibody (AB154898)

Immunofluorescent analysis of methanol-fixed HepG2 cells labeling CRALBP with ab154898 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P12271

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'CRALBP' also known as cellular retinaldehyde-binding protein is a protein involved in the visual cycle. It weighs approximately 36 kDa. Expressed mainly in the retina CRALBP is found in retinal pigment epithelial (RPE) cells and Muller glial cells. It binds to retinoids which are critical for proper vision functioning. CRALBP also goes by the synonyms such as 'BSA b 2' which is used in specific contexts related to its role in retinal metabolism.
Biological function summary

The protein aids in the effective conversion of all-trans-retinal to 11-cis-retinal an important step in the visual cycle. It acts as a chaperone that stabilizes retinoid intermediates facilitating their transport between various retinal cell types. CRALBP forms part of a complex with retinoid isomerohydrolase and 11-cis-retinol dehydrogenase enhancing its binding and transport function. This ensures smooth retinoid flux necessary for regenerating the visual pigment rhodopsin.

Pathways

CRALBP is integral to two main pathways; the retinoid cycle in cones and the rhodopsin regeneration pathway in rods. In these pathways it interacts with proteins such as RPE65 and 11-cis-retinol dehydrogenase ensuring efficient retinoid transfer and isomerization. This important involvement supports the regeneration of visual pigments necessary for photoreceptor cell function under various lighting conditions.

CRALBP associates with retina-related conditions notably congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP). Mutations affecting CRALBP can alter retinoid transport and contribute to these disorders. Additionally CRALBP dysfunction links to other proteins like RPE65 and RDH5 which can also play roles in the pathogenesis of these visual impairments. Understanding these relationships helps in developing targeted treatments for retinoid-related visual disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
See full target information RLBP1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 25: PubMed32987810

2020

Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ayyad Zartasht Khan,Catherine Joan Jackson,Tor Paaske Utheim,Sjur Reppe,Dipak Sapkota,Ole Kristoffer Olstad,Bernd Thiede,Jon Roger Eidet

Frontiers in cellular neuroscience 14:7 PubMed32082123

2020

Optimization of a Method to Isolate and Culture Adult Porcine, Rats and Mice Müller Glia in Order to Study Retinal Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Xandra Pereiro,Noelia Ruzafa,Arantxa Acera,Aritz Urcola,Elena Vecino

PloS one 14:e0221344 PubMed31419250

2019

Aeromonas sobria serine protease decreases epithelial barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hidetomo Kobayashi,Soshi Seike,Masafumi Yamaguchi,Mitsunobu Ueda,Eizo Takahashi,Keinosuke Okamoto,Hiroyasu Yamanaka

Autophagy 14:1796-1817 PubMed29979914

2018

Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Sriganesh Ramachandra Rao,Bruce A Pfeffer,Néstor Más Gómez,Lara A Skelton,Ueda Keiko,Janet R Sparrow,Aryn M Rowsam,Claire H Mitchell,Steven J Fliesler
View all publications
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Product promise

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