Rabbit Polyclonal CRALBP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus.
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- Molecules (Basel, Switzerland) 25:2020Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyyad Zartasht Khan et. al.PubMed 32987810
- Frontiers in cellular neuroscience 14:72020Optimization of a Method to Isolate and Culture Adult Porcine, Rats and Mice Müller Glia in Order to Study Retinal Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXandra Pereiro et. al.PubMed 32082123
- Autophagy 14:1796-18172018Compromised phagosome maturation underlies RPE pathology in cell culture and whole animal models of Smith-Lemli-Opitz Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSriganesh Ramachandra Rao et. al.PubMed 29979914
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12271
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
Alternative names
CRALBP, RLBP1, Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein
Recommended products
Rabbit Polyclonal CRALBP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RLBP1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P12271
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'CRALBP' also known as cellular retinaldehyde-binding protein is a protein involved in the visual cycle. It weighs approximately 36 kDa. Expressed mainly in the retina CRALBP is found in retinal pigment epithelial (RPE) cells and Muller glial cells. It binds to retinoids which are critical for proper vision functioning. CRALBP also goes by the synonyms such as 'BSA b 2' which is used in specific contexts related to its role in retinal metabolism.
- Biological function summary
The protein aids in the effective conversion of all-trans-retinal to 11-cis-retinal an important step in the visual cycle. It acts as a chaperone that stabilizes retinoid intermediates facilitating their transport between various retinal cell types. CRALBP forms part of a complex with retinoid isomerohydrolase and 11-cis-retinol dehydrogenase enhancing its binding and transport function. This ensures smooth retinoid flux necessary for regenerating the visual pigment rhodopsin.
- Pathways
CRALBP is integral to two main pathways; the retinoid cycle in cones and the rhodopsin regeneration pathway in rods. In these pathways it interacts with proteins such as RPE65 and 11-cis-retinol dehydrogenase ensuring efficient retinoid transfer and isomerization. This important involvement supports the regeneration of visual pigments necessary for photoreceptor cell function under various lighting conditions.
- Associated diseases and disorders
CRALBP associates with retina-related conditions notably congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP). Mutations affecting CRALBP can alter retinoid transport and contribute to these disorders. Additionally CRALBP dysfunction links to other proteins like RPE65 and RDH5 which can also play roles in the pathogenesis of these visual impairments. Understanding these relationships helps in developing targeted treatments for retinoid-related visual disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CRALBP antibody (ab154898)
10% SDS PAGE
All lanes: Western blot - Anti-CRALBP antibody (ab154898) at 1/3000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CRALBP antibody (ab154898)
Immunofluorescent analysis of methanol-fixed HepG2 cells labeling CRALBP with ab154898 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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