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AB183728

Anti-CRALBP antibody [EPR13801]

4

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal CRALBP antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CRALBP, RLBP1, Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein

2 Images
Immunoprecipitation - Anti-CRALBP antibody [EPR13801] (AB183728)
  • IP

Supplier Data

Immunoprecipitation - Anti-CRALBP antibody [EPR13801] (AB183728)

Western blot analysis of immunoprecipitation pellet from mouse eyeball lysate immunoprecipitated using ab183728 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-CRALBP antibody [EPR13801] (ab183728)

Predicted band size: 36 kDa

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Western blot - Anti-CRALBP antibody [EPR13801] (AB183728)
  • WB

Supplier Data

Western blot - Anti-CRALBP antibody [EPR13801] (AB183728)

All lanes:

Western blot - Anti-CRALBP antibody [EPR13801] (ab183728) at 1/5000 dilution

Lane 1:

mouse eyeball lysate at 10 µg

Lane 2:

rat eyeball lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

false

  • Carrier free

    Anti-CRALBP antibody [EPR13801] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13801

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'CRALBP' also known as cellular retinaldehyde-binding protein is a protein involved in the visual cycle. It weighs approximately 36 kDa. Expressed mainly in the retina CRALBP is found in retinal pigment epithelial (RPE) cells and Muller glial cells. It binds to retinoids which are critical for proper vision functioning. CRALBP also goes by the synonyms such as 'BSA b 2' which is used in specific contexts related to its role in retinal metabolism.
Biological function summary

The protein aids in the effective conversion of all-trans-retinal to 11-cis-retinal an important step in the visual cycle. It acts as a chaperone that stabilizes retinoid intermediates facilitating their transport between various retinal cell types. CRALBP forms part of a complex with retinoid isomerohydrolase and 11-cis-retinol dehydrogenase enhancing its binding and transport function. This ensures smooth retinoid flux necessary for regenerating the visual pigment rhodopsin.

Pathways

CRALBP is integral to two main pathways; the retinoid cycle in cones and the rhodopsin regeneration pathway in rods. In these pathways it interacts with proteins such as RPE65 and 11-cis-retinol dehydrogenase ensuring efficient retinoid transfer and isomerization. This important involvement supports the regeneration of visual pigments necessary for photoreceptor cell function under various lighting conditions.

CRALBP associates with retina-related conditions notably congenital stationary night blindness (CSNB) and retinitis pigmentosa (RP). Mutations affecting CRALBP can alter retinoid transport and contribute to these disorders. Additionally CRALBP dysfunction links to other proteins like RPE65 and RDH5 which can also play roles in the pathogenesis of these visual impairments. Understanding these relationships helps in developing targeted treatments for retinoid-related visual disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.
See full target information RLBP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 59: PubMed34779489

2021

Increased expression of PSME2 is associated with clear cell renal cell carcinoma invasion by regulating BNIP3‑mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Wang,Fengbo Wu,Yutong Deng,Jinlong Chai,Yuehua Zhang,Gu He,Xiang Li

Molecular pharmacology 98:1-12 PubMed32362584

2020

Small-Molecule Ligands that Bind the RET Receptor Activate Neuroprotective Signals Independent of but Modulated by Coreceptor GFR1.

Applications

Unspecified application

Species

Unspecified reactive species

Sean Jmaeff,Yulia Sidorova,Hayley Lippiatt,Pablo F Barcelona,Hinyu Nedev,Lucia M Saragovi,Mark A Hancock,Mart Saarma,H Uri Saragovi

The Journal of biological chemistry 295:6532-6542 PubMed32245892

2020

Small-molecule agonists of the RET receptor tyrosine kinase activate biased trophic signals that are influenced by the presence of GFRa1 co-receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Sean Jmaeff,Yulia Sidorova,Hinyu Nedev,Mart Saarma,H Uri Saragovi
View all publications
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Product promise

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