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AB238501

Anti-CRB1 antibody

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Rabbit Polyclonal CRB1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRB1 aa 1-200.

View Alternative Names

Protein crumbs homolog 1, CRB1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB1 antibody (AB238501)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB1 antibody (AB238501)

Paraffin-embedded human eye tissue stained for CRB1 using ab238501 at 1/66 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-CRB1 antibody (AB238501)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRB1 antibody (AB238501)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CRB1 (Green) using ab238501 at 1/22 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CRB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P82279

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRB1 also known as Crumbs Homolog 1 is a protein with a mass of approximately 160 kDa. Mechanically CRB1 plays a critical role in the structural maintenance of retinal photoreceptor cells. It localizes predominantly to the apical membrane of these cells in the retina. CRB1 helps organize intercellular junctions and maintain cell polarity providing essential support to the retina's architecture and function.
Biological function summary

CRB1 maintains cell polarity and adhesion in photoreceptors which are critical for normal vision function. It is part of the CRB1 complex which includes other proteins such as CRB2 and CRB3. This complex operates within the retina and the brain maintaining the structural integrity necessary for cellular communication and overall tissue organization.

Pathways

CRB1 plays a role in the Hippo signaling pathway and the cellular polarity regulation pathway. In these pathways it interacts with proteins such as MPP5 and AMOT which are integral for regulating cell growth and maintaining tissue homeostasis. These interactions ensure proper functioning and survival of retinal cells preventing their degradation over time.

CRB1 mutations associate with retinal dystrophies including retinitis pigmentosa and Leber congenital amaurosis. These conditions lead to progressive vision loss due to the degeneration of photoreceptor cells. Mutations can lead to dysfunctional interaction with proteins like PALS1 and CRB3 disrupting the cell polarity and junction maintenance which ultimately affects the structural support in the retina.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in photoreceptor morphogenesis in the retina (By similarity). May maintain cell polarization and adhesion (By similarity).
See full target information CRB1
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Product promise

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