Rabbit Polyclonal CRB1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRB1 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CRB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P82279
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a role in photoreceptor morphogenesis in the retina (By similarity). May maintain cell polarization and adhesion (By similarity).
Alternative names
Protein crumbs homolog 1, CRB1
Recommended products
Rabbit Polyclonal CRB1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRB1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CRB1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P82279
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRB1 also known as Crumbs Homolog 1 is a protein with a mass of approximately 160 kDa. Mechanically CRB1 plays a critical role in the structural maintenance of retinal photoreceptor cells. It localizes predominantly to the apical membrane of these cells in the retina. CRB1 helps organize intercellular junctions and maintain cell polarity providing essential support to the retina's architecture and function.
- Biological function summary
CRB1 maintains cell polarity and adhesion in photoreceptors which are critical for normal vision function. It is part of the CRB1 complex which includes other proteins such as CRB2 and CRB3. This complex operates within the retina and the brain maintaining the structural integrity necessary for cellular communication and overall tissue organization.
- Pathways
CRB1 plays a role in the Hippo signaling pathway and the cellular polarity regulation pathway. In these pathways it interacts with proteins such as MPP5 and AMOT which are integral for regulating cell growth and maintaining tissue homeostasis. These interactions ensure proper functioning and survival of retinal cells preventing their degradation over time.
- Associated diseases and disorders
CRB1 mutations associate with retinal dystrophies including retinitis pigmentosa and Leber congenital amaurosis. These conditions lead to progressive vision loss due to the degeneration of photoreceptor cells. Mutations can lead to dysfunctional interaction with proteins like PALS1 and CRB3 disrupting the cell polarity and junction maintenance which ultimately affects the structural support in the retina.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB1 antibody (ab238501)
Paraffin-embedded human eye tissue stained for CRB1 using ab238501 at 1/66 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-CRB1 antibody (ab238501)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CRB1 (Green) using ab238501 at 1/22 dilution in ICC/IF, followed by Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
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