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AB126373

Anti-CRB2 antibody

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(1 Publication)

Rabbit Polyclonal CRB2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CRB2.

View Alternative Names

Protein crumbs homolog 2, Crumbs-like protein 2, CRB2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

Immunohistochemical analysis of human eye tissue (retina) labelling CRB2 in outer limiting membrane with ab126373 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

Immunohistochemical analysis of human Fallopian tube tissue labelling CRB2 in apical membrane of glandular cells with ab126373 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

Immunohistochemical analysis of human placenta tissue labelling CRB2 in membrane of trophoblastic cells with ab126373 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

Immunohistochemical analysis of human cerebral cortex labelling CRB2 in cytoplasm of neuronal cells with ab126373 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

Immunohistochemical analysis of human kidney tissue labelling CRB2 in membrane of cells in glomeruli with ab126373 at 1/50 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CRB2. The exact immunogen used to generate this antibody is proprietary information.

Q5IJ48

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRB2 also known as Crumbs homolog 2 is a protein involved in important cellular functions. It is part of the Crumbs family and has a molecular weight of approximately 137 kDa. Researchers find CRB2 expression largely in the brain and kidneys playing roles in maintaining cell junction integrity. The protein localizes mainly to tight junctions where it plays a mechanically important role in stabilizing cell polarity and tissue organization.
Biological function summary

CRB2 contributes to processes of epithelial cell polarization and morphogenesis. As part of a protein complex involving CRB1 CRB2 and CRB3 it participates in maintaining cell cortex stability. Through this action CRB2 ensures that the cellular architecture remains intact which is essential for proper organ function and development. It influences the formation of apical-basal polarity in epithelial cells which is essential for constructing specialized tissues.

Pathways

CRB2 integrates into the Hippo signaling pathway and tight junction pathway. In the Hippo pathway it closely interacts with proteins like YAP and TAZ to regulate organ size by controlling cell proliferation and apoptosis. By affecting tight junctions CRB2 also influences pathways involved in cell adhesion and the maintenance of cellular barriers connecting with partners such as ZO-1.

CRB2 associates with retinal degeneration and nephrotic syndrome. Mutations affecting its function can lead to retinopathies as its role in maintaining retinal cell structure is compromised. Similarly CRB2's interaction with podocyte proteins is critical in kidney function linking it to nephrotic syndrome when dysfunctional. Pathological conditions also show connections to CRB1 where mutations in both proteins may combine to exacerbate disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo (By similarity). Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer (By similarity). The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress : cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB (By similarity). Plays a role in the maintenance of retinal neuroepithelium organization, structural integrity, adhesion, photoreceptor polarity and retinal photoreceptor layer thickness (By similarity). May play a role in determining the length of cone photoreceptor outer segments and proliferation of late-born progenitor cells (By similarity). Also required for maintenance of the apical polarity complex during development of the cortex (By similarity). Inhibits gamma-secretase-dependent cleavage of APP and secretion of amyloid-beta peptide 40 and amyloid-beta peptide 42, and thereby inhibits gamma-secretase-dependent Notch transcription (PubMed : 20299451).
See full target information CRB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadp4765 PubMed39841844

2025

WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by loss of function.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Wang,Rui Zhu,Pengcheng Yu,Sixian Qi,Zhenxing Zhong,Ruxin Jin,Yu Wang,Yuan Gu,Dan Ye,Kang Chen,Yilai Shu,Yi Wang,Fa-Xing Yu
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