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AB156286

Anti-CRB2 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal CRB2 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CRB2 aa 450-550.

View Alternative Names

Protein crumbs homolog 2, Crumbs-like protein 2, CRB2

2 Images
Western blot - Anti-CRB2 antibody (AB156286)
  • WB

Supplier Data

Western blot - Anti-CRB2 antibody (AB156286)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRB2 antibody (ab156286) at 1 µg/mL

All lanes:

Human Kidney Tissue Lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 100 kDa,123 kDa,134 kDa

false

Western blot - Anti-CRB2 antibody (AB156286)
  • WB

Supplier Data

Western blot - Anti-CRB2 antibody (AB156286)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRB2 antibody (ab156286) at 1 µg/mL

Lane 1:

Mouse Brain Tissue Lysate at 15 µg

Lane 2:

Rat Brain Tissue Lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 100 kDa,123 kDa,134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CRB2 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q5IJ48

Specificity

At least three isoforms of CRB2 are known to exist; ab156286 will detect all three. This antibody is predicted to not crossreact with other members of the CRB family.

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Alternative Products

Primary Antibodies

AB126373

Anti-CRB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB2 antibody (AB126373)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB254715

Anti-ZNF503 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF503 antibody (AB254715)

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Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

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Primary Antibodies

AB238501

Anti-CRB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRB1 antibody (AB238501)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRB2 also known as Crumbs homolog 2 is a protein involved in important cellular functions. It is part of the Crumbs family and has a molecular weight of approximately 137 kDa. Researchers find CRB2 expression largely in the brain and kidneys playing roles in maintaining cell junction integrity. The protein localizes mainly to tight junctions where it plays a mechanically important role in stabilizing cell polarity and tissue organization.
Biological function summary

CRB2 contributes to processes of epithelial cell polarization and morphogenesis. As part of a protein complex involving CRB1 CRB2 and CRB3 it participates in maintaining cell cortex stability. Through this action CRB2 ensures that the cellular architecture remains intact which is essential for proper organ function and development. It influences the formation of apical-basal polarity in epithelial cells which is essential for constructing specialized tissues.

Pathways

CRB2 integrates into the Hippo signaling pathway and tight junction pathway. In the Hippo pathway it closely interacts with proteins like YAP and TAZ to regulate organ size by controlling cell proliferation and apoptosis. By affecting tight junctions CRB2 also influences pathways involved in cell adhesion and the maintenance of cellular barriers connecting with partners such as ZO-1.

CRB2 associates with retinal degeneration and nephrotic syndrome. Mutations affecting its function can lead to retinopathies as its role in maintaining retinal cell structure is compromised. Similarly CRB2's interaction with podocyte proteins is critical in kidney function linking it to nephrotic syndrome when dysfunctional. Pathological conditions also show connections to CRB1 where mutations in both proteins may combine to exacerbate disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo (By similarity). Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer (By similarity). The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress : cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB (By similarity). Plays a role in the maintenance of retinal neuroepithelium organization, structural integrity, adhesion, photoreceptor polarity and retinal photoreceptor layer thickness (By similarity). May play a role in determining the length of cone photoreceptor outer segments and proliferation of late-born progenitor cells (By similarity). Also required for maintenance of the apical polarity complex during development of the cortex (By similarity). Inhibits gamma-secretase-dependent cleavage of APP and secretion of amyloid-beta peptide 40 and amyloid-beta peptide 42, and thereby inhibits gamma-secretase-dependent Notch transcription (PubMed : 20299451).
See full target information CRB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:8959-8974 PubMed32469452

2020

PTPN22 interacts with EB1 to regulate T-cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Yang Yu,Bin Bai,Tao Wang,Jiahui Zhao,Na Zhang,Yanjiao Zhao,Xipeng Wang,Bing Wang
View all publications
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Product promise

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