JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB180835

Anti-CRB3 antibody [14F9]

2

(1 Review)

|

(7 Publications)

Rat Monoclonal CRB3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CRB3.

View Alternative Names

UNQ588/PRO1158, CRB3, Protein crumbs homolog 3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (AB180835)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (AB180835)

Immunofluorescence analysis of HeLa cell labeling CRB3 with ab1808350 at a 1/100 dilution. DAPI was used to stain the nuclei.

Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (AB180835)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRB3 antibody [14F9] (AB180835)

Immunofluorescence analysis of Mouse intestine labeling CRB3 with ab1808350 at a 1/100 dilution. E-cadherin staining was used to highlight the lateral membrane in mouse intestine.

Western blot - Anti-CRB3 antibody [14F9] (AB180835)
  • WB

Supplier Data

Western blot - Anti-CRB3 antibody [14F9] (AB180835)

All lanes:

Western blot - Anti-CRB3 antibody [14F9] (ab180835) at 1/1000 dilution

Lane 1:

HEK293T cells overexpressed with control vector

Lane 2:

HEK293T cells overexpressed with CRB3

Lane 3:

Mouse kidney tissue lysate

Lane 4:

Mouse colon tissue lysate

Predicted band size: 13 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

14F9

Isotype

IgG2c

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CRB3.

Q9BUF7

Specificity

The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Lyophilized protein G purified in PBS pH7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CRB3 also known as Crumbs homolog 3 is an essential component in maintaining cell polarity and tight junction formation. With a mass of approximately 13-15 kDa CRB3 is a small but important member of the Crumbs family of proteins. It is expressed in epithelial tissues especially across the apical membrane of epithelial cells contributing to the structural organization and proper functioning of tight junctions. CRB3 is integral in ensuring the polarized distribution of cellular components therefore supporting epithelial barrier function.
Biological function summary

In epithelial cells CRB3 plays a role in establishing and preserving apical-basal polarity. It functions as part of a complex with PALS1 and PATJ important for maintaining tight junction structures. CRB3 interacts with the actin cytoskeleton through interactions with other proteins and modulates cellular adhesion properties. Through such activities CRB3 contributes to cell morphology and prevents the invasion of pathogens across epithelial barriers.

Pathways

CRB3 serves a pivotal role in pathways regulating epithelial cell polarity and tissue homeostasis. CRB3 is connected with the AMPK signaling pathway and interacts with other proteins like LKB1 and STRAD. This connection influences cellular energy balance and metabolism important for the survival and function of polarized cells. CRB3 regulates processes within these pathways that control how epithelial cells respond to metabolic stress and maintain homeostasis.

Mutation or dysfunction in CRB3 is associated with epithelial integrity issues contributing to diseases like cancer and retinal dystrophy. In cancer loss of CRB3 function can result in disrupted cell polarity enhanced tumor invasiveness and metastasis. CRB3's involvement with the RhoA pathway links it to changes in cell morphology and motility further playing a role in cancer pathophysiology. In retinal dystrophy CRB3 interacts with proteins like CRB1 where mutations in these proteins affect photoreceptor structure and function leading to vision impairment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the establishment of cell polarity in mammalian epithelial cells (PubMed : 12771187, PubMed : 14718572, PubMed : 23439680). Regulates the morphogenesis of tight junctions (PubMed : 12771187, PubMed : 14718572). Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity).
See full target information CRB3
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115509 PubMed40186871

2025

The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Sherry,Komal Ishwar Pawar,Lee Dolat,Erin Smith,I-Chang Chang,Khavong Pha,Robyn Kaake,Danielle L Swaney,Clara Herrera,Eleanor McMahon,Robert J Bastidas,Jeffrey R Johnson,Raphael H Valdivia,Nevan J Krogan,Cherilyn A Elwell,Kliment Verba,Joanne N Engel

Scientific reports 14:5707 PubMed38459197

2024

Proteomic analysis of serum extracellular vesicles from biliary tract infection patients to identify novel biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Jung Chang,Yung-Ning Huang,Yang-Bor Lu,Yi Zhang,Ping-Hua Wu,Jian-Shan Huang,Wei Yang,Tung-Ying Chiang,Hui-Shan Hsieh,Wen-Hung Chung,Yu-Chieh Weng

American journal of physiology. Renal physiology 324:F211-F224 PubMed36546837

2022

Transcription factor HNF1β controls a transcriptional network regulating kidney cell structure and tight junction integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Lotte E Tholen,Femke Latta,Joost H A Martens,Joost G J Hoenderop,Jeroen H F de Baaij

Nature genetics 51:1389-1398 PubMed31477929

2019

A pooled single-cell genetic screen identifies regulatory checkpoints in the continuum of the epithelial-to-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

José L McFaline-Figueroa,Andrew J Hill,Xiaojie Qiu,Dana Jackson,Jay Shendure,Cole Trapnell

Cellular microbiology 19: PubMed28618099

2017

EPEC effector EspF promotes Crumbs3 endocytosis and disrupts epithelial cell polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Tapia,Sarah E Kralicek,Gail A Hecht

EMBO molecular medicine 9:890-905 PubMed28500065

2017

Loss of Mpdz impairs ependymal cell integrity leading to perinatal-onset hydrocephalus in mice.

Applications

ICC/IF

Species

Mouse

Anja Feldner,M Gordian Adam,Fabian Tetzlaff,Iris Moll,Dorde Komljenovic,Felix Sahm,Tobias Bäuerle,Hiroshi Ishikawa,Horst Schroten,Thomas Korff,Ilse Hofmann,Hartwig Wolburg,Andreas von Deimling,Andreas Fischer

Molecular medicine reports 14:2555-65 PubMed27484148

2016

Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis.

Applications

IHC

Species

Unspecified reactive species

Chao Huang,Hong Liu,Xiuli Gong,Bin Wen,Dan Chen,Jinyuan Liu,Fengliang Hu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

  • 5
  • 67

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com