Knockout Tested Mouse Monoclonal CRBN antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img101152.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81638.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776)")
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img435339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776)")
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img419610.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (AB252776)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Species Mouse | Dilution info 2 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL (PubMed:26990986, PubMed:33009960). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity). Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling (PubMed:31620128).
Alternative names
AD-006, CRBN, Protein cereblon
Recommended products
Knockout Tested Mouse Monoclonal CRBN antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 4D6
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRBN also known as cereblon is a protein involved in the regulation of protein homeostasis. It has a molecular weight of approximately 51 kDa. CRBN is expressed in various tissues including the brain kidney and muscle. It functions as a substrate receptor for the CRL4-CRBN E3 ubiquitin ligase complex recognizing proteins for ubiquitination and degradation. The chemical structure of thalidomide shows binding affinity to CRBN influencing its function.
- Biological function summary
Cereblon plays a role in the regulation of protein stability and degradation. It forms part of the CRL4-CRBN complex which tags specific proteins for ubiquitination and subsequent proteasomal degradation. This function is critical for maintaining cellular protein balance. Additionally CRBN modulates immune response and has immunomodulator properties influencing cell-mediated responses.
- Pathways
CRBN participates in the ubiquitin-proteasome pathway which regulates protein turnover and quality control within cells. Moreover CRBN is involved in immune signaling pathways partly by modulating the expression of various cytokines and immune-related proteins. It interacts with proteins such as DDB1 and CUL4 within these pathways impacting cellular responses to stress and immune challenges.
- Associated diseases and disorders
CRBN is associated with multiple myeloma and intellectual disability. Cereblon acts as a target for drugs like thalidomide and lenalidomide which are used in the treatment of multiple myeloma by altering ubiquitination processes. In intellectual disability alterations in CRBN function or expression can disrupt neuronal development and signaling. CRBN interacts with proteins like IKZF1 and IKZF3 within these contexts affecting their roles in disease progression and treatment responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img101152.jpg "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)")
Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CRBN antibody [4D6] ab244223).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-CRBN antibody [4D6] ab244223 observed at 55 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
Anti-CRBN antibody [4D6] ab244223 was shown to react with CRBN in MOLT4 (Licensor sample) wild-type cells in Western blot. Loss of signal was observed when CRBN knockout sample was used. MOLT4 (Licensor sample) wild-type and CRBN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-CRBN antibody [4D6] ab244223 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 2 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CRBN antibody [4D6] (Anti-CRBN antibody [4D6] ab244223) at 2 µg/mL
Lane 1: wild-type MOLT4 (Licensor sample) cell lysate at 20 µg
Lane 2: CRBN knockout MOLT4 cell lysate at 20 µg
Lane 3: NIH/3T3 cell lysate at 20 µg
Lane 4: MEF-1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81638.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)
This image was developed using the same antibody clone but in a different formulation, PBS and sodium azide, Anti-CRBN antibody [4D6] ab244223.
IHC image of CRBN staining in a section of formalin-fixed paraffin-embedded normal human testis* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-CRBN antibody [4D6] ab244223, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img435339.jpg "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)")
Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)
This data was developed using the same antibody clone in a different buffer formulation (abAB244223).
Western blot: Anti-CRBN antibody [4D6] (Anti-CRBN antibody [4D6] ab244223) staining at 0.2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-CRBN antibody [4D6] ab244223 was shown to bind specifically to CRBN. A band was observed at 51 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CRBN knockout cell line. To generate this image, wild-type and CRBN knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CRBN antibody [4D6] (Anti-CRBN antibody [4D6] ab244223) at 0.2 µg/mL
Lane 1: Wild-type MCF7 cell lysate at 20 µg
Lane 2: CRBN knockout MCF7 cell lysate at 20 µg
Lane 3: Wild-type A549 ab288558 cell lysate at 20 µg
Lane 4: CRBN knockout A549 ab288959 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 51 kDa
![Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776), expandable thumbnail](https://content.abcam.com/products/images/crbn-antibody-4d6-bsa-and-azide-free-ab252776--western-blot-img419610.jpg "Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)")
Western blot - Anti-CRBN antibody [4D6] - BSA and Azide free (ab252776)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CRBN antibody [4D6] ab244223).
Anti-CRBN antibody [4D6] (Anti-CRBN antibody [4D6] ab244223) staining at 2 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CRBN antibody [4D6] ab244223 was shown to bind specifically to CRBN. A band was observed at 51 kDa in wild-type cell lysates with no signal observed at this size in CRBN knockout cell lines. To generate this image, wild-type and CRBN knockout cell lysates were analysed: CRBN knockout A549 cell line (ab288959), CRBN knockout HCT 116 cell line (ab288959) and CRBN knockout MOLT-4. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CRBN antibody [4D6] (Anti-CRBN antibody [4D6] ab244223) at 2 µg/mL
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: Western blot - Human CRBN knockout A549 cell line (ab288959)
Lane 2: CRBN knockout A549 cell lysate at 20 µg
Lane 3: Wild-type HCT 116 cell lysate at 20 µg
Lane 4: Western blot - Human CRBN knockout HCT116 cell line (Human CRBN knockout HCT116 cell line ab289186)
Lane 4: CRBN knockout HCT 116 cell lysate at 20 µg
Lane 5: Wild-type MOLT-4 cell lysate at 20 µg
Lane 6: CRBN knockout MOLT-4 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
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