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Rabbit Recombinant Monoclonal CRBN antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Immunoprecipitation - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (AB315345), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (AB315345), expandable thumbnail
  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (AB315345), expandable thumbnail
  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (AB315345), expandable thumbnail
  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (AB315345), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIPFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Tested
Expected
Expected

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL (PubMed:26990986, PubMed:33009960). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity). Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling (PubMed:31620128).

Alternative names

AD-006, CRBN, Protein cereblon

Recommended products

Rabbit Recombinant Monoclonal CRBN antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28739-64
Purification technique
Affinity purification Protein A
Specificity

WB-rat is labelled with inconclusive, since the autoubiquitination of CRBN (around 100 kDa; PMID: 25043012) are found in both cell & tissue WB. There is slightly different from WB-human & mouse.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315345 is the carrier-free version of Anti-CRBN antibody [EPR28739-64] ab315344.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CRBN also known as cereblon is a protein involved in the regulation of protein homeostasis. It has a molecular weight of approximately 51 kDa. CRBN is expressed in various tissues including the brain kidney and muscle. It functions as a substrate receptor for the CRL4-CRBN E3 ubiquitin ligase complex recognizing proteins for ubiquitination and degradation. The chemical structure of thalidomide shows binding affinity to CRBN influencing its function.

Biological function summary

Cereblon plays a role in the regulation of protein stability and degradation. It forms part of the CRL4-CRBN complex which tags specific proteins for ubiquitination and subsequent proteasomal degradation. This function is critical for maintaining cellular protein balance. Additionally CRBN modulates immune response and has immunomodulator properties influencing cell-mediated responses.

Pathways

CRBN participates in the ubiquitin-proteasome pathway which regulates protein turnover and quality control within cells. Moreover CRBN is involved in immune signaling pathways partly by modulating the expression of various cytokines and immune-related proteins. It interacts with proteins such as DDB1 and CUL4 within these pathways impacting cellular responses to stress and immune challenges.

Associated diseases and disorders

CRBN is associated with multiple myeloma and intellectual disability. Cereblon acts as a target for drugs like thalidomide and lenalidomide which are used in the treatment of multiple myeloma by altering ubiquitination processes. In intellectual disability alterations in CRBN function or expression can disrupt neuronal development and signaling. CRBN interacts with proteins like IKZF1 and IKZF3 within these contexts affecting their roles in disease progression and treatment responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345), expandable thumbnail

    Immunoprecipitation - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345)

    This data was developed using Anti-CRBN antibody [EPR28739-64] ab315344, the same antibody clone in a different buffer formulation.

    CRBN was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-CRBN antibody [EPR28739-64] ab315344 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CRBN antibody [EPR28739-64] ab315344 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-CRBN antibody [EPR28739-64] ab315344 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CRBN antibody [EPR28739-64] ab315344 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-CRBN antibody [EPR28739-64] (Anti-CRBN antibody [EPR28739-64] ab315344) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345)

    This data was developed using Anti-CRBN antibody [EPR28739-64] ab315344, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CRBN with Anti-CRBN antibody [EPR28739-64] ab315344 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345), expandable thumbnail

    Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345)

    This data was developed using Anti-CRBN antibody [EPR28739-64] ab315344, the same antibody clone in a different buffer formulation.

    The molecular weight observed around 100 kDa is autoubiquitination of CRBN, which is consistent with what has been described in the literature (PMID: 25043012).

    Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-9 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CRBN antibody [EPR28739-64] (Anti-CRBN antibody [EPR28739-64] ab315344) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human testis tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 7: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 8: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 9: Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/100000 dilution

    Lanes 4 - 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 3s

  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345), expandable thumbnail

    Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345)

    This data was developed using Anti-CRBN antibody [EPR28739-64] ab315344, the same antibody clone in a different buffer formulation.

    The molecular weight observed around 100 kDa is autoubiquitination of CRBN, which is consistent with what has been described in the literature (PMID: 25043012).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CRBN antibody [EPR28739-64] (Anti-CRBN antibody [EPR28739-64] ab315344) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 3: U266B1 (human mtiple myeloma B lymphocyte) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 5: HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 7: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 8: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 9: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 40 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 5.5s

  • Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345), expandable thumbnail

    Western blot - Anti-CRBN antibody [EPR28739-64] - BSA and Azide free (ab315345)

    This data was developed using Anti-CRBN antibody [EPR28739-64] ab315344, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CRBN antibody [EPR28739-64] (Anti-CRBN antibody [EPR28739-64] ab315344) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting CRBN whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

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