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Rabbit Recombinant Monoclonal CRCP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873), expandable thumbnail
  • Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873), expandable thumbnail
  • Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.

Alternative names

DNA-directed RNA polymerase III subunit RPC9, RNA polymerase III subunit C9, Calcitonin gene-related peptide-receptor component protein, CGRP-RCP, CGRP-receptor component protein, CGRPRCP, HsC17, CRCP

Recommended products

Rabbit Recombinant Monoclonal CRCP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Alternative names

DNA-directed RNA polymerase III subunit RPC9, RNA polymerase III subunit C9, Calcitonin gene-related peptide-receptor component protein, CGRP-RCP, CGRP-receptor component protein, CGRPRCP, HsC17, CRCP

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR9670(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248873 is the carrier-free version of Anti-CRCP antibody [EPR9670(B)] ab139264.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target CRCP also known as CGRP receptor component protein is a protein with a mass of approximately 148 kDa. It has a mechanical role in assisting the function of calcitonin gene-related peptide (CGRP) receptors. CRCP localizes in various tissues including the central nervous system and peripheral tissues like the heart and kidneys. Its expression is important for the proper signaling of peptide hormones in these areas.

Biological function summary

The CRCP influences the efficacy of CGRP receptor signaling. It functions as a part of the receptor complex aiding in the transmission of signals that control vasodilation and neuronal communication. CRCP does not act alone often associating with other components such as receptor activity-modifying proteins (RAMPs) and calcitonin receptor-like receptors (CLR) forming a fully functional CGRP receptor complex. This complex regulates important processes like blood flow and neurotransmitter release.

Pathways

CRCP participates in pathways related to neurovascular and endocrine signaling. It plays a role in the CGRP signaling pathway which involves significant proteins such as RAMP1 and CLR. These pathways contribute to the regulation of blood vessel tone and neuropeptide release impacting various physiological responses. CRCP's role in these pathways highlights its importance in maintaining proper cardiovascular and nervous system functioning.

Associated diseases and disorders

CRCP is involved with migraine and cardiovascular diseases. Aberrations in CRCP or its associated signaling pathways may lead to dysregulation contributing to migraine pathophysiology. The protein's interaction with CGRP is well-documented in these conditions where the overactivation of the CGRP pathway can exacerbate symptoms. Moreover since CRCP is part of the vascular regulation mechanism any disruption in its function might contribute to cardiovascular disorders indicating a significant therapeutic target in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRCP antibody [EPR9670(B)] (Anti-CRCP antibody [EPR9670(B)] ab139264) at 1/5000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 17 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRCP antibody [EPR9670(B)] (Anti-CRCP antibody [EPR9670(B)] ab139264) at 1/10000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 17 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CRCP antibody [EPR9670(B)] (Anti-CRCP antibody [EPR9670(B)] ab139264) at 1/1000 dilution

    Lane 1: Human ovary cancer lysate at 10 µg

    Lane 2: HeLa lysate at 10 µg

    Lane 3: Jurkat lysate at 10 µg

    Lane 4: Human prostate cancer lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Anti-CRCP antibody [EPR9670(B)] ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (Anti-CRCP antibody [EPR9670(B)] ab139264)

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Anti-CRCP antibody [EPR9670(B)] ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (Anti-CRCP antibody [EPR9670(B)] ab139264)

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (ab248873)

    This data was developed using Anti-CRCP antibody [EPR9670(B)] ab139264, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified Anti-CRCP antibody [EPR9670(B)] ab139264 at 1/100.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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