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AB248873

Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CRCP antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

DNA-directed RNA polymerase III subunit RPC9, RNA polymerase III subunit C9, Calcitonin gene-related peptide-receptor component protein, CGRP-RCP, CGRP-receptor component protein, CGRPRCP, HsC17, CRCP

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IP

Lab

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (<a href='/en-us/products/primary-antibodies/crcp-antibody-epr9670b-ab139264'>ab139264</a>)

Predicted band size: 17 kDa

false

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • IP

Lab

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-CRCP antibody [EPR9670(B)] (<a href='/en-us/products/primary-antibodies/crcp-antibody-epr9670b-ab139264'>ab139264</a>)

Predicted band size: 17 kDa

false

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • WB

Unknown

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRCP antibody [EPR9670(B)] (<a href='/en-us/products/primary-antibodies/crcp-antibody-epr9670b-ab139264'>ab139264</a>) at 1/1000 dilution

Lane 1:

Human ovary cancer lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Jurkat lysate at 10 µg

Lane 4:

Human prostate cancer lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa

false

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • WB

Lab

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRCP antibody [EPR9670(B)] (<a href='/en-us/products/primary-antibodies/crcp-antibody-epr9670b-ab139264'>ab139264</a>) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 20 kDa

false

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)
  • WB

Lab

Western blot - Anti-CRCP antibody [EPR9670(B)] - BSA and Azide free (AB248873)

This data was developed using ab139264, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRCP antibody [EPR9670(B)] (<a href='/en-us/products/primary-antibodies/crcp-antibody-epr9670b-ab139264'>ab139264</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9670(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248873 is the carrier-free version of ab139264.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CRCP also known as CGRP receptor component protein is a protein with a mass of approximately 148 kDa. It has a mechanical role in assisting the function of calcitonin gene-related peptide (CGRP) receptors. CRCP localizes in various tissues including the central nervous system and peripheral tissues like the heart and kidneys. Its expression is important for the proper signaling of peptide hormones in these areas.
Biological function summary

The CRCP influences the efficacy of CGRP receptor signaling. It functions as a part of the receptor complex aiding in the transmission of signals that control vasodilation and neuronal communication. CRCP does not act alone often associating with other components such as receptor activity-modifying proteins (RAMPs) and calcitonin receptor-like receptors (CLR) forming a fully functional CGRP receptor complex. This complex regulates important processes like blood flow and neurotransmitter release.

Pathways

CRCP participates in pathways related to neurovascular and endocrine signaling. It plays a role in the CGRP signaling pathway which involves significant proteins such as RAMP1 and CLR. These pathways contribute to the regulation of blood vessel tone and neuropeptide release impacting various physiological responses. CRCP's role in these pathways highlights its importance in maintaining proper cardiovascular and nervous system functioning.

CRCP is involved with migraine and cardiovascular diseases. Aberrations in CRCP or its associated signaling pathways may lead to dysregulation contributing to migraine pathophysiology. The protein's interaction with CGRP is well-documented in these conditions where the overactivation of the CGRP pathway can exacerbate symptoms. Moreover since CRCP is part of the vascular regulation mechanism any disruption in its function might contribute to cardiovascular disorders indicating a significant therapeutic target in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed : 20413673, PubMed : 33558764, PubMed : 34675218). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With POLR3H/RPC8 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation (By similarity) (PubMed : 20413673, PubMed : 33558764, PubMed : 33558766, PubMed : 34675218). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed : 19609254, PubMed : 19631370).. Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.
See full target information CRCP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com