Anti-Cre recombinase antibody is a rabbit polyclonal antibody that is used to detect Cre recombinase in ICC/IF, Western blot. Suitable for Bacteriophage P1 samples.
- Cre recombinanse, an enzyme from the P1 bacteriophage catalyzes site-specific recombination between two 34-base-pair loxP sites
- Cited in over 10 publications
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Publications
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- iScience 26:1063382023Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction.Applications:IHC-PReactive species:MouseHuanjuan Su,Zhichen Yang,Ying Zhang,Bihui Yang,Zhanmei Zhou,Cailing Su,Wei CaoPubMed 36968090
- Experimental neurology 360:1142892022Ischemic stroke rehabilitation through optogenetic modulation of parvalbumin neurons in the contralateral motor cortex.Applications:IFReactive species:Unspecified reactive speciesWeilin Liu,Xiaojun He,Huawei Lin,Minguang Yang,Yaling Dai,Lewen Chen,Chaohui Li,Shengxiang Liang,Jing Tao,Lidian ChenPubMed 36471512
- Reproduction (Cambridge, England) 163:199-2072022Desmoplakin (Dsp) conditional knockout in NR5A1+ somatic cells affects germ cell survival in developing mouse gonads.Applications:IHCReactive species:MouseRafal P Piprek,Izabela Rams-Pociecha,Robert Zdanowski,Malgorzata Kloc,Jacek Z KubiakPubMed 35119382
- JID innovations : skin science from molecules to population health 2:1000762022A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A KarrethPubMed 35146482
- Brain research bulletin 170:174-1862021Longitudinal tracing of neurochemical metabolic disorders in working memory neural circuit and optogenetics modulation in rats with vascular cognitive impairment.Applications:IFReactive species:RatHuawei Lin,Tingting Jin,Lewen Chen,Yaling Dai,Weiwei Jia,Xiaojun He,Minguang Yang,Jianhong Li,Shengxiang Liang,Jinsong Wu,Jia Huang,Lidian Chen,Weilin Liu,Jing TaoPubMed 33600886
- Methods (San Diego, Calif.) :2020A high-throughput screening platform to identify nanocarriers for efficient delivery of RNA-based therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVitor Francisco et. al.PubMed 33359052
- American journal of physiology. Heart and circulat 319:H443-H4552020The role of endothelial autocrine NRG1/ERBB4 signaling in cardiac remodeling.
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 35:956-9652020Ezh2 Ameliorates Osteoarthritis by Activating TNFSF13B.Applications:IHC-PReactive species:MouseXiaotian Du,Yishan Chen,Qin Zhang,Junxin Lin,Yeke Yu,Zongyou Pan,Heng Sun,Chunhui Yuan,Dongsheng Yu,Haoyu Wu,Xiaoan Zhang,Jun Dai,Shouan Zhu,Yiting Zhou,Hongwei OuyangPubMed 31910305
- Cells 8:2019N-Cadherin Is Critical for the Survival of Germ Cells, the Formation of Steroidogenic Cells, and the Architecture of Developing Mouse Gonads.
- Cell reports 29:1579-1593.e62019Spontaneous Pain Disrupts Ventral Hippocampal CA1-Infralimbic Cortex Connectivity and Modulates Pain Progression in Rats with Peripheral Inflammation.Applications:IHCReactive species:RatLongyu Ma,Lupeng Yue,Yuqi Zhang,Yue Wang,Bingxuan Han,Shuang Cui,Feng-Yu Liu,You Wan,Ming YiPubMed 31693897
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Bacteriophage P1 Recombinase cre. Database link P06956
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Bacteriophage P1 | Predicted | Predicted |
| Transfected cell line - Bacteriophage P1 | Not recommended | Tested |
| Transfected cell lysate - Bacteriophage P1 | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Bacteriophage P1 | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bacteriophage P1 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Bacteriophage P1 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Bacteriophage P1 | Dilution info 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Bacteriophage P1 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Bacteriophage P1 | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
Additional Targets
Cre recombinase
Alternative names
Recombinase cre, cre
Recommended products
Anti-Cre recombinase antibody is a rabbit polyclonal antibody that is used to detect Cre recombinase in ICC/IF, Western blot. Suitable for Bacteriophage P1 samples.
- Cre recombinanse, an enzyme from the P1 bacteriophage catalyzes site-specific recombination between two 34-base-pair loxP sites
- Cited in over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Bacteriophage P1 Recombinase cre. Database link P06956
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Cre recombinase antibody (ab190177) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in bacteriophage p1 samples.
Anti-Cre recombinase antibody (ab190177) specifically detects Cre recombinase (UniProt ID: P06956; Molecular weight: 39kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Cre recombinase antibody (ab190177) has high sensitivity and specificity.
Anti-Cre recombinase antibody (ab190177) has been cited over 16 times in peer reviewed journals and is trusted by the scientific community.
Anti-Cre recombinase antibody (ab190177) has 7 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (ab190177)
Immunofluorescent analysis of U-2 OS cells (Human bone osteosarcoma epithelial cell line) transfected with GFP-Cre recombinase (green) labeling Cre recombinase with ab190177 at 1/3000 dilution (red). DAPI staining is shown in blue.
Western blot - Anti-Cre recombinase antibody (ab190177)
Predicted band size : 26 kDa (GFP) 38 kDa (Cre)= 64 kDa
WB: 40 μg of whole cell extracts transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ +4°C in 5% milk TBST.
All lanes: Western blot - Anti-Cre recombinase antibody (ab190177) at 1/2000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 40 µg
Lane 2: HEK-293T cells transfected with GFP-Cre recombinase lysate at 40 µg
Predicted band size: 39 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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