AB190177

Anti-Cre recombinase antibody

Name: Anti-Cre recombinase antibody, rabbit polyclonal (ab190177) | Abcam
Brand: Abcam Limited
SKU: AB190177
Price: 470 USD
Availability: InStock
Rating: 3 (10 reviews)

(10 Reviews)

(22 Publications)

Anti-Cre recombinase antibody (ab190177) is a rabbit polyclonal antibody detecting Cre recombinase in Western Blot, ICC/IF. Suitable for Bacteriophage P1.

- Over 10 publications

View Alternative Names

Recombinase cre, cre, Cre, Recombinase cre, Cyclization recombinase

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB190177)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB190177)

Immunofluorescent analysis of U-2 OS cells (Human bone osteosarcoma epithelial cell line) transfected with GFP-Cre recombinase (green) labeling Cre recombinase with ab190177 at 1/3000 dilution (red). DAPI staining is shown in blue.

Supplier Data

Western blot - Anti-Cre recombinase antibody (AB190177)

Predicted band size : 26 kDa (GFP) 38 kDa (Cre)= 64 kDa

WB : 40 μg of whole cell extracts transfer in nitrocelullose, block : 1 hour 5% milk in TBST, incubation with primary antibody o/n @ +4°C in 5% milk TBST.

All lanes:

Western blot - Anti-Cre recombinase antibody (ab190177) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 40 µg

Lane 2:

HEK-293T cells transfected with GFP-Cre recombinase lysate at 40 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Bacteriophage P1

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Bacteriophage P1 Recombinase cre.

P06956

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacteriophage P1": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Bacteriophage P1": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/3000", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Bacteriophage P1": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Product Specifications
Anti-Cre recombinase antibody (ab190177) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in bacteriophage p1 samples.
Anti-Cre recombinase antibody (ab190177) specifically detects Cre recombinase (UniProt ID: P06956; Molecular weight: 39kDa) and is sold in 100 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Cre recombinase antibody (ab190177) has high sensitivity and specificity.
Anti-Cre recombinase antibody (ab190177) has been cited over 16 times in peer reviewed journals and is trusted by the scientific community.
Anti-Cre recombinase antibody (ab190177) has 7 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.

See full target information Recombinase cre

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1818-1836 PubMed40813470

2025

Dysregulation of FGFR1 signaling in the hippocampus facilitates depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Jongpil Shin,Hyeonsik Oh,Ji Hye Park,Hyunsik Bae,Chan Mo Yang,Minju Lee,Seokhwi Kim,Won Do Heo

mSphere 9:e0075024 PubMed39526784

2024

Nuclear pore complexes undergo Nup221 exchange during blood-stage asexual replication of parasites.

Applications

Unspecified application

Species

Unspecified reactive species

James Blauwkamp,Sushma V Ambekar,Tahir Hussain,Gunnar R Mair,Josh R Beck,Sabrina Absalon

Science advances 10:eadj4433 PubMed38959322

2024

Phospholipase C beta 1 in the dentate gyrus gates fear memory formation through regulation of neuronal excitability.

Applications

Unspecified application

Species

Unspecified reactive species

Jinsu Lee,Yeonji Jeong,Seahyung Park,Sungsoo Kim,Hyunsik Oh,Ju-Ae Jin,Jong-Woo Sohn,Daesoo Kim,Hee-Sup Shin,Won Do Heo

Nature communications 15:3953 PubMed38729967

2024

Fused in sarcoma (FUS) inhibits milk production efficiency in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Haili Shao,Jipeng Huang,Hui Wang,Guolei Wang,Xu Yang,Mei Cheng,Changjie Sun,Li Zou,Qin Yang,Dandan Zhang,Zhen Liu,Xuelong Jiang,Lei Shi,Peng Shi,Baowei Han,Baowei Jiao

Science advances 10:eadk7636 PubMed38728397

2024

Hyperexcitation of ovBNST CRF neurons during stress contributes to female-biased expression of anxiety-like avoidance behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Na Zhang,Sha Zhao,Yanqiao Ma,Zhixin Xiao,Bao Xue,Yuan Dong,Qingyu Wang,Huamin Xu,Xia Zhang,Ying Wang

iScience 26:106338 PubMed36968090

2023

Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction.

Applications

IHC-P

Species

Mouse

Huanjuan Su,Zhichen Yang,Ying Zhang,Bihui Yang,Zhanmei Zhou,Cailing Su,Wei Cao

Experimental neurology 360:114289 PubMed36471512

2022

Ischemic stroke rehabilitation through optogenetic modulation of parvalbumin neurons in the contralateral motor cortex.

Applications

Species

Unspecified reactive species

Weilin Liu,Xiaojun He,Huawei Lin,Minguang Yang,Yaling Dai,Lewen Chen,Chaohui Li,Shengxiang Liang,Jing Tao,Lidian Chen

Stem cell research & therapy 13:160 PubMed35410459

2022

Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.

Applications

IHC-FrFl

Species

Mouse

Hsin-Hsiung Chen,Hao-Yu Lu,Chao-Hsin Chang,Shih-Hao Lin,Chu-Wei Huang,Po-Han Wei,Yi-Wen Chen,Yi-Rou Lin,Hsien-Sung Huang,Pei-Yu Wang,Yeou-Ping Tsao,Show-Li Chen

Reproduction (Cambridge, England) 163:199-207 PubMed35119382

2022

Desmoplakin (Dsp) conditional knockout in NR5A1+ somatic cells affects germ cell survival in developing mouse gonads.

Applications

IHC

Species

Mouse

Rafal P Piprek,Izabela Rams-Pociecha,Robert Zdanowski,Malgorzata Kloc,Jacek Z Kubiak

JID innovations : skin science from molecules to population health 2:100076 PubMed35146482

2022

A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Ilah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A Karreth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cre recombinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB190177)

Western blot - Anti-Cre recombinase antibody (AB190177)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (22)

Product promise