Anti-Cre recombinase antibody (ab216262) is a rabbit polyclonal antibody that is used to detect Cre recombinase in Western Blot, Flow Cytometry, ICC/IF. Suitable for Bacteriophage P1 samples.
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- Genes & diseases 11:964-9772023deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling.Applications:IHC-FrReactive species:Unspecified reactive speciesChengzhi Wu,Hengyu Liu,Dongmei Zhong,Xiaoming Yang,Zhiheng Liao,Yuyu Chen,Shun Zhang,Deying Su,Baolin Zhang,Chuan Li,Liru Tian,Caixia Xu,Peiqiang SuPubMed 37692479
- Glia 71:450-4662022Astrocytic Yin Yang 1 is critical for murine brain development and protection against apoptosis, oxidative stress, and inflammation.Applications:IHCReactive species:Unspecified reactive speciesEdward Pajarillo,Ivan Nyarko-Danquah,Alexis Digman,Cynthia Vied,Deok-Soo Son,Jayden Lee,Michael Aschner,Eunsook LeePubMed 36300569
- Glia 70:1886-19012022Deletion of RE1-silencing transcription factor in striatal astrocytes exacerbates manganese-induced neurotoxicity in mice.Applications:IHCReactive species:Unspecified reactive speciesEdward Pajarillo,Mark Demayo,Alexis Digman,Ivan Nyarko-Danquah,Deok-Soo Son,Michael Aschner,Eunsook LeePubMed 35638297
- Nature cell biology 24:645-6582022Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElif Eroglu,Christopher Y T Yen,Yat-Long Tsoi,Nevin Witman,Ahmed Elewa,Alberto Joven Araus,Heng Wang,Tamara Szattler,Chimezie H Umeano,Jesper Sohlmér,Alexander Goedel,András Simon,Kenneth R ChienPubMed 35550612
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Bacteriophage P1 Recombinase cre aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P06956
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Reactivity data
Select an application| ICC/IF | WB | Flow Cyt | |
|---|---|---|---|
| Bacteriophage P1 | Expected | Expected | Tested |
| Recombinant fragment | Tested | Tested | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1/1000 - 1/2000 | Notes Use antigen retrieval with microwave boiling for 7 minutes in 10mM Tris HCl, pH 9. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Bacteriophage P1 | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Bacteriophage P1 | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Bacteriophage P1 | Dilution info 1/200 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
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Target data
Function
Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
Additional Targets
Cre recombinase
Alternative names
Recombinase cre, cre
Recommended products
Anti-Cre recombinase antibody (ab216262) is a rabbit polyclonal antibody that is used to detect Cre recombinase in Western Blot, Flow Cytometry, ICC/IF. Suitable for Bacteriophage P1 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Bacteriophage P1 Recombinase cre aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P06956
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Cre recombinase antibody (ab216262) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Bacteriophage P1 samples.
What is the molecular weight of Cre recombinase?
Anti-Cre recombinase (ab216262) specifically detects a band for Cre recombinase (UniProt: P06956) at a molecular weight of 39kDa.
Recommended positive controls
293T transfected with GFP-Cre recombinase; Human 293T cells transfected with GFP-Cre recombinase lysate;
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-Cre recombinase antibody (ab216262)
All lanes: Western blot - Anti-Cre recombinase antibody (ab216262) at 1/2000 dilution
Lane 1: Human 293T cell lysate at 40 µg
Lane 2: Human 293T cells transfected with GFP-Cre recombinase lysate at 40 µg
Predicted band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (ab216262)
Immunocytochemistry analysis of formalin fixed and paraffin-embedded 239T transfected with empty vector (left) or with GFP-Cre recombinase (right) labeling Cre recombinase using ab216262 at 1/1000 dilution, after antigen retrieval.
Flow Cytometry - Anti-Cre recombinase antibody (ab216262)
Flow cytometric analysis of formalin fixed 293T non transfected (top) or transfected with GFP-Cre recombinase (bottom) labeling Cre recombinase using ab216262 at 1/200 dilution, followed by Alexa 647 conjugated secondary antibody detection.
Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (ab216262)
Immunofluorescent analysis of formalin fixed 293T cells transfected with GFP-Cre recombinase (green) and incubated with ab216262 at 1/2000 dilution (red). DAPI staining is shown in blue.
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