AB216262

Anti-Cre recombinase antibody

Name: Anti-Cre recombinase antibody (ab216262) | Abcam
Brand: Abcam Limited
SKU: AB216262
Rating: 4 (2 reviews)

(2 Reviews)

(8 Publications)

Anti-Cre recombinase antibody (ab216262) is a rabbit polyclonal antibody detecting Cre recombinase in Western Blot, Flow Cytometry, ICC/IF. Suitable for Bacteriophage P1.

View Alternative Names

Recombinase cre, cre

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

Immunocytochemistry analysis of formalin fixed and paraffin-embedded 239T transfected with empty vector (left) or with GFP-Cre recombinase (right) labeling Cre recombinase using ab216262 at 1/1000 dilution, after antigen retrieval.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

Immunofluorescent analysis of formalin fixed 293T cells transfected with GFP-Cre recombinase (green) and incubated with ab216262 at 1/2000 dilution (red). DAPI staining is shown in blue.

Flow Cytometry - Anti-Cre recombinase antibody (AB216262)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cre recombinase antibody (AB216262)

Flow cytometric analysis of formalin fixed 293T non transfected (top) or transfected with GFP-Cre recombinase (bottom) labeling Cre recombinase using ab216262 at 1/200 dilution, followed by Alexa 647 conjugated secondary antibody detection.

Supplier Data

Western blot - Anti-Cre recombinase antibody (AB216262)

All lanes:

Western blot - Anti-Cre recombinase antibody (ab216262) at 1/2000 dilution

Lane 1:

Human 293T cell lysate at 40 µg

Lane 2:

Human 293T cells transfected with GFP-Cre recombinase lysate at 40 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Bacteriophage P1

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Bacteriophage P1 Recombinase cre aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P06956

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacteriophage P1": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "" }, "Recombinant fragment": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "Use antigen retrieval with microwave boiling for 7 minutes in 10mM Tris HCl, pH 9.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Cre recombinase antibody (ab216262) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Bacteriophage P1 samples.

What is the molecular weight of Cre recombinase?
Anti-Cre recombinase (ab216262) specifically detects a band for Cre recombinase (UniProt: P06956) at a molecular weight of 39kDa.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.

See full target information Recombinase cre

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Virchows Archiv : an international journal of pathology 486:803-815 PubMed39592484

2024

Low CXCL11 expression is indicative of poor prognosis in rectal cancer patients undergoing preoperative chemoradiotherapy: a retrospective cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Lin Chou,Cheng-Yi Lin,Wan-Shan Li,Sung-Wei Lee,Ching-Chieh Yang,Yu-Feng Tian,Yow-Ling Shiue,Hsin-Hwa Tsai,Hong-Yue Lai

Frontiers in neuroscience 17:1241919 PubMed37869509

2023

An experimental platform for stochastic analyses of single serotonergic fibers in the mouse brain.

Applications

IHC-P

Species

Mouse

Kasie C Mays,Justin H Haiman,Skirmantas Janušonis

American journal of physiology. Renal physiology 325:F770-F778 PubMed37823193

2023

Generation of -Cre mice for investigation of intercalated cells and the collecting duct.

Applications

IHC-P

Species

Human

Vijay Saxena,Samuel Arregui,Shaobo Zhang,Jorge Canas,Xuebin Qin,David S Hains,Andrew L Schwaderer

Genes & diseases 11:964-977 PubMed37692479

2023

deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling.

Applications

IHC-Fr

Species

Unspecified reactive species

Chengzhi Wu,Hengyu Liu,Dongmei Zhong,Xiaoming Yang,Zhiheng Liao,Yuyu Chen,Shun Zhang,Deying Su,Baolin Zhang,Chuan Li,Liru Tian,Caixia Xu,Peiqiang Su

The American journal of pathology 193:493-508 PubMed36638912

2023

Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxu Yuan,Owais M Bhat,Yao Zou,Yang Zhang,Pin-Lan Li

Glia 71:450-466 PubMed36300569

2022

Astrocytic Yin Yang 1 is critical for murine brain development and protection against apoptosis, oxidative stress, and inflammation.

Applications

IHC

Species

Unspecified reactive species

Edward Pajarillo,Ivan Nyarko-Danquah,Alexis Digman,Cynthia Vied,Deok-Soo Son,Jayden Lee,Michael Aschner,Eunsook Lee

Glia 70:1886-1901 PubMed35638297

2022

Deletion of RE1-silencing transcription factor in striatal astrocytes exacerbates manganese-induced neurotoxicity in mice.

Applications

IHC

Species

Unspecified reactive species

Edward Pajarillo,Mark Demayo,Alexis Digman,Ivan Nyarko-Danquah,Deok-Soo Son,Michael Aschner,Eunsook Lee

Nature cell biology 24:645-658 PubMed35550612

2022

Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders.

Applications

Unspecified application

Species

Unspecified reactive species

Elif Eroglu,Christopher Y T Yen,Yat-Long Tsoi,Nevin Witman,Ahmed Elewa,Alberto Joven Araus,Heng Wang,Tamara Szattler,Chimezie H Umeano,Jesper Sohlmér,Alexander Goedel,András Simon,Kenneth R Chien

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cre recombinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

Flow Cytometry - Anti-Cre recombinase antibody (AB216262)

Western blot - Anti-Cre recombinase antibody (AB216262)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

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