JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240304

Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Cre recombinase antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein samples. Cited in 1 publication.

View Alternative Names

Recombinase cre, cre

1 Images
Western blot - Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (AB240304)
  • WB

Supplier Data

Western blot - Anti-Cre recombinase antibody [EPR13835] - BSA and Azide free (AB240304)

This data was developed using ab188568, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cre recombinase antibody [EPR13835] (<a href='/en-us/products/primary-antibodies/cre-recombinase-antibody-epr13835-ab188568'>ab188568</a>) at 1/5000 dilution

Lane 1:

Active bacteriophage p1 Cre recombinase full length protein at 0.001 µg

Lane 1:

Western blot - Recombinant Bacteriophage P1 Cre recombinase protein (<a href='/en-us/products/proteins-peptides/recombinant-bacteriophage-p1-cre-recombinase-protein-ab134845'>ab134845</a>) at 0.001 µg

Lane 2:

E. coli at 10 µg

Lane 3:

Saccharomyces cerevisiae at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa,86 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13835

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab240304 is the carrier-free version of ab188568.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
See full target information Recombinase cre
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 54:455-470.e5 PubMed32553121

2020

DDX3X Suppresses the Susceptibility of Hindbrain Lineages to Medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deanna M Patmore,Amir Jassim,Erica Nathan,Reuben J Gilbertson,Daniel Tahan,Nadin Hoffmann,Yiai Tong,Kyle S Smith,Thirumala-Devi Kanneganti,Hiromichi Suzuki,Michael D Taylor,Paul Northcott,Richard J Gilbertson
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB190177

Anti-Cre recombinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB190177)

  • 3
  • 10
Primary Antibodies

AB216262

Anti-Cre recombinase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cre recombinase antibody (AB216262)

  • 4
  • 2

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB252881

Anti-GFP antibody [3H9]

BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)

  • 0
  • 0
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com