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Rabbit Polyclonal Creatine kinase B type antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250.


Images

Western blot - Anti-Creatine kinase B type antibody (AB151579), expandable thumbnail
  • Western blot - Anti-Creatine kinase B type antibody (AB151579), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (AB151579), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody (AB151579), expandable thumbnail
  • Western blot - Anti-Creatine kinase B type antibody (AB151579), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P12277

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Creatine kinase B type antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P12277
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Creatine kinase B type antibody (ab151579), expandable thumbnail

    Western blot - Anti-Creatine kinase B type antibody (ab151579)

    10% SDS PAGE

    All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

    All lanes: H1299 cell lysate at 30 µg

    Predicted band size: 43 kDa

  • Western blot - Anti-Creatine kinase B type antibody (ab151579), expandable thumbnail

    Western blot - Anti-Creatine kinase B type antibody (ab151579)

    10% SDS PAGE

    All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

    All lanes: NIH-3T3 cell lysate at 30 µg

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (ab151579), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (ab151579)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Creatine kinase B with ab151579 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody (ab151579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody (ab151579)

    Immunohistochemical analysis of paraffin-embedded human hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.

  • Western blot - Anti-Creatine kinase B type antibody (ab151579), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Creatine kinase B type antibody (ab151579)

    All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

    Lane 1: Xenopus laevis brain tissue lysate - whole at 15 µg

    Lane 2: HEK293 cell lysate at 15 µg

    Lane 3: Rat brain tissue lysate - whole at 15 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 43 kDa, 75-135 kDa

    Exposure time: 30s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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