Rabbit Polyclonal Creatine kinase B type antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow, Pig | Dilution info - | Notes - |
Select an associated product type
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
CKBB, CKBB, CKB, Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK, CPK-B
Rabbit Polyclonal Creatine kinase B type antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Creatine kinase B-type aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein A
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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10% SDS PAGE
All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution
All lanes: H1299 cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE
All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution
All lanes: NIH-3T3 cell lysate at 30 µg
Predicted band size: 43 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Creatine kinase B with ab151579 at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded human hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.
All lanes: Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution
Lane 1: Xenopus laevis brain tissue lysate - whole at 15 µg
Lane 2: HEK293 cell lysate at 15 µg
Lane 3: Rat brain tissue lysate - whole at 15 µg
All lanes: HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa, 75-135 kDa
Exposure time: 30s
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