Anti-Creatine kinase B type antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody (AB151579)

Immunohistochemical analysis of paraffin-embedded human hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (AB151579)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Creatine kinase (brain) protein at cytoplasm and nucleus using ab151579 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody (AB151579)

Immunofluorescence analysis of 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling Creatine kinase (brain) protein at cytoplasm using ab151579 at a 1/1000 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

• WB

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Western blot - Anti-Creatine kinase B type antibody (AB151579)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Lane 3:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

• WB

AbReview44420****

Western blot - Anti-Creatine kinase B type antibody (AB151579)

All lanes:

Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

Lane 1:

Xenopus laevis brain tissue lysate - whole at 15 µg

Lane 2:

HEK293 cell lysate at 15 µg

Lane 3:

Rat brain tissue lysate - whole at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa,75-135 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-Creatine kinase B type antibody (AB151579)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

• WB

Supplier Data

Western blot - Anti-Creatine kinase B type antibody (AB151579)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

All lanes:

Zebrafish eye tissue lysate at 30 µg

Predicted band size: 43 kDa

false