AB125114

Anti-Creatine kinase B type antibody [CK-BYK/21E10]

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(7 Publications)

Mouse Monoclonal Creatine kinase B type antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CKB aa 1-50.

View Alternative Names

CKBB, CKB, Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK, CPK-B

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Lab

Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (AB125114)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Creatine kinase B knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab125114 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab125114 was shown to recognize Creatine kinase B when Creatine kinase B knockout samples were used, along with additional cross-reactive bands. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab125114 and ab181602 (loading control to GAPDH) were diluted 1/50 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW)preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)

Predicted band size: 43 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CK-BYK/21E10

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human CKB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P12277

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed : 8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle : localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

See full target information CKB

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3221 PubMed40185737

2025

The Futile Creatine Cycle powers UCP1-independent thermogenesis in classical BAT.

Applications

Unspecified application

Species

Unspecified reactive species

Jakub Bunk,Mohammed F Hussain,Maria Delgado-Martin,Bozena Samborska,Mina Ersin,Abhirup Shaw,Janane F Rahbani,Lawrence Kazak

Biology 12: PubMed37508386

2023

Mitochondrial Proteome Changes in Rett Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Gocha Golubiani,Laura van Agen,Lia Tsverava,Revaz Solomonia,Michael Müller

Neoplasia (New York, N.Y.) 23:1147-1165 PubMed34706306

2021

CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Mohit Hulsurkar,Lijuan Zhuo,Jinbang Xu,Han Yang,Samira Naderinezhad,Lin Wang,Guoliang Zhang,Nanping Ai,Linna Li,Jeffrey T Chang,Songlin Zhang,Ladan Fazli,Chad J Creighton,Fang Bai,Michael M Ittmann,Martin E Gleave,Wenliang Li

Frontiers in neuroscience 13:1234 PubMed31803010

2019

Investigations Into Bioenergetic Neuroprotection of Cone Photoreceptors: Relevance to Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel S Narayan,Glyn Chidlow,John P M Wood,Robert J Casson

FEBS letters 593:601-610 PubMed30801684

2019

Possible ATP trafficking by ATP-shuttles in the olfactory cilia and glucose transfer across the olfactory mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Acevedo,Kris Blanchard,Juan Bacigalupo,Cecilia Vergara

Nature communications 9:4770 PubMed30425250

2018

Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Shangwen Jiang,Min Li,Xuelian Xiong,Mingrui Zhu,Duanzhuo Li,Lei Zhao,Lili Qian,Linhui Zhai,Jing Li,Han Lu,Shengnan Sun,Jiandie Lin,Yan Lu,Xiaoying Li,Minjia Tan

Proceedings of the National Academy of Sciences of 110:19820-5 PubMed24248342

2013

Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Louise E Glover,Brittelle E Bowers,Bejan Saeedi,Stefan F Ehrentraut,Eric L Campbell,Amanda J Bayless,Evgenia Dobrinskikh,Agnieszka A Kendrick,Caleb J Kelly,Adrianne Burgess,Lauren Miller,Douglas J Kominsky,Paul Jedlicka,Sean P Colgan

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-Creatine kinase B type antibody [CK-BYK/21E10]

Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (AB125114)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise