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AB125114

Anti-Creatine kinase B type antibody [CK-BYK/21E10]

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(5 Publications)

Mouse Monoclonal Creatine kinase B type antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CKB aa 1-50.

View Alternative Names

CKBB, CKB, Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK, CPK-B

1 Images
Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (AB125114)
  • WB

Lab

Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (AB125114)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Creatine kinase B knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab125114 observed at 45 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab125114 was shown to recognize Creatine kinase B when Creatine kinase B knockout samples were used, along with additional cross-reactive bands. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab125114 and ab181602 (loading control to GAPDH) were diluted 1/50 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW)preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Creatine kinase B type antibody [CK-BYK/21E10] (ab125114)

Predicted band size: 43 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CK-BYK/21E10

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CKB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P12277

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Reactivity data

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Have you thought about this alternative?

AB108388

Anti-Creatine kinase B type antibody [EPR3926]

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We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99% PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed : 8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle : localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
See full target information CKB
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biology 12: PubMed37508386

2023

Mitochondrial Proteome Changes in Rett Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Gocha Golubiani,Laura van Agen,Lia Tsverava,Revaz Solomonia,Michael Müller

Neoplasia (New York, N.Y.) 23:1147-1165 PubMed34706306

2021

CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Mohit Hulsurkar,Lijuan Zhuo,Jinbang Xu,Han Yang,Samira Naderinezhad,Lin Wang,Guoliang Zhang,Nanping Ai,Linna Li,Jeffrey T Chang,Songlin Zhang,Ladan Fazli,Chad J Creighton,Fang Bai,Michael M Ittmann,Martin E Gleave,Wenliang Li

Frontiers in neuroscience 13:1234 PubMed31803010

2019

Investigations Into Bioenergetic Neuroprotection of Cone Photoreceptors: Relevance to Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel S Narayan,Glyn Chidlow,John P M Wood,Robert J Casson

Nature communications 9:4770 PubMed30425250

2018

Proteome-wide analysis of USP14 substrates revealed its role in hepatosteatosis via stabilization of FASN.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Shangwen Jiang,Min Li,Xuelian Xiong,Mingrui Zhu,Duanzhuo Li,Lei Zhao,Lili Qian,Linhui Zhai,Jing Li,Han Lu,Shengnan Sun,Jiandie Lin,Yan Lu,Xiaoying Li,Minjia Tan

Proceedings of the National Academy of Sciences of 110:19820-5 PubMed24248342

2013

Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Louise E Glover,Brittelle E Bowers,Bejan Saeedi,Stefan F Ehrentraut,Eric L Campbell,Amanda J Bayless,Evgenia Dobrinskikh,Agnieszka A Kendrick,Caleb J Kelly,Adrianne Burgess,Lauren Miller,Douglas J Kominsky,Paul Jedlicka,Sean P Colgan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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