Anti-Creatine kinase B type antibody [EPR3926]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Creatine kinase B type with purified ab108388 at 1/500 dilution (1.14 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Lab

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Creatine kinase B knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108388 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab108388 was shown to specifically react with Creatine kinase B when Creatine kinase B knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab108388 and ab18058 (loading control to vinculin) were diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)

Predicted band size: 43 kDa

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• WB

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Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

SH SY5Y cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 43 kDa

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• WB

Lab

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)

All lanes:

Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

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