Rabbit Recombinant Monoclonal Creatine kinase B type antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Images
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img18016.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)")
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img9044.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104145.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)")
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img71570.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (AB108388)")
Publications
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- The EMBO journal 42:e1111482023A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingxin Zhu,Yi Tang,Xiao-Yan Li,Samuel A Kerk,Costas A Lyssiotis,Wenqing Feng,Xiaoyue Sun,Geoffrey E Hespe,Zijun Wang,Marc P Stemmler,Simone Brabletz,Thomas Brabletz,Evan T Keller,Jun Ma,Jung-Sun Cho,Jingwen Yang,Stephen J WeissPubMed 36843552
- American journal of cancer research 12:4652-46652022CKB affects human osteosarcoma progression by regulating the p53 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengxin Zhou,Xinli Dou,Chenguang LiPubMed 36381321
- Cell death & disease 12:10502021Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenyang Duan,Lei Kuang,Chen Hong,Xinming Xiang,Jiancang Liu,Qinghui Li,Xiaoyong Peng,Yuanqun Zhou,Hongchen Wang,Liangming Liu,Tao LiPubMed 34741026
- Neoplasia (New York, N.Y.) 23:1147-11652021CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Wang,Mohit Hulsurkar,Lijuan Zhuo,Jinbang Xu,Han Yang,Samira Naderinezhad,Lin Wang,Guoliang Zhang,Nanping Ai,Linna Li,Jeffrey T Chang,Songlin Zhang,Ladan Fazli,Chad J Creighton,Fang Bai,Michael M Ittmann,Martin E Gleave,Wenliang LiPubMed 34706306
- Andrology 8:407-4202019Characterization of seminal plasma proteomic alterations associated with the IVF and rescue-ICSI pregnancy in assisted reproduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesX Liu,G Liu,P Zhu,Y Wang,J Wang,W Zhang,W Wang,N Li,X Wang,C Zhang,J Liu,X Shen,F LiuPubMed 31364287
- Oncology letters 18:1133-11442019Cystic and solitary nodular pulmonary metastases in a patient with low-grade endometrial stromal sarcoma: A case report and literature review.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Xu et. al.PubMed 31423173
- Molecular reproduction and development 83:526-402016Implications of caveolae in testicular and epididymal myoid cells to sperm motility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRegiana L Oliveira,Adam Parent,Daniel G Cyr,Mary Gregory,Craig A Mandato,Charles E Smith,Louis HermoPubMed 27088550
- Proceedings of the National Academy of Sciences of 110:19820-52013Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLouise E Glover et. al.PubMed 24248342
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/100 - 1/250. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50000 - 1/200000 | Notes - |
Species Rat | Dilution info 1/50000 - 1/200000 | Notes - |
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
Alternative names
CKBB, CKB, Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK, CPK-B
Recommended products
Rabbit Recombinant Monoclonal Creatine kinase B type antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3926
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img18016.jpg "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)")
Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
All lanes: Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: Mouse brain lysates at 20 µg
Lane 3: Rat brain lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img9044.jpg "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)")
Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: SH-SY5Y cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108388 observed at 45 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab108388 was shown to specifically react with Creatine kinase B when Creatine kinase B knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab108388 and ab18058 (loading control to vinculin) were diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
Predicted band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104145.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Creatine kinase B type with purified ab108388 at 1/500 dilution (1.14 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--western-blot-img71570.jpg "Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)")
Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
All lanes: Western blot - Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: SH SY5Y cell lysate at 10 µg
Lane 3: Y79 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Predicted band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-b-type-antibody-epr3926-ab108388--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3926] (ab108388)
ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.
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