Mouse Monoclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-mm-antibody-3e5ba5-ab110319--western-blot-img5621.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)")
![Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-mm-antibody-3e5ba5-ab110319--western-blot-img417463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Alternative names
CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M
Recommended products
Mouse Monoclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 3E5BA5
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
- Purification notes
ab110319 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-mm-antibody-3e5ba5-ab110319--western-blot-img5621.jpg "Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)")
Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)
All lanes: Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/mL
All lanes: Human heart homogenate at 20 µg
Predicted band size: 43 kDa
![Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319), expandable thumbnail](https://content.abcam.com/products/images/creatine-kinase-mm-antibody-3e5ba5-ab110319--western-blot-img417463.jpg "Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)")
Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)
Creatine Kinase MM Western blot staining using mouse Anti-Creatine Kinase MM antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 was used as a loading control.All lanes: Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1/50 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Mouse placenta tissue lysate at 20 µg
Lane 4: Human skeletal muscle tissue lysate at 20 µg
Lane 5: Human spleen tissue lysate at 20 µg
Lane 6: Human placenta tissue lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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