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AB110319

Anti-Creatine Kinase MM antibody [3E5BA5]

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(1 Publication)

Mouse Monoclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

2 Images
Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)
  • WB

Lab

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab129002 was used as a loading control.

All lanes:

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1/50 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse placenta tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Lane 5:

Human spleen tissue lysate at 20 µg

Lane 6:

Human placenta tissue lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Exposure time: 180s

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)
  • WB

Unknown

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319)

All lanes:

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/mL

All lanes:

Human heart homogenate at 20 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E5BA5

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab110319 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information CKM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106831 PubMed37250791

2023

Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hu Zhao,Peinan Zhao,Zihao Deng,Ting Yang,Yu-Xing Qi,Li-Ya An,Da-Li Sun,Hai-Yu He
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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