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Mouse Monoclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319), expandable thumbnail
  • Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (AB110319), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Alternative names

Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M, CKMM, CKM

Recommended products

Mouse Monoclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

3E5BA5

Purification technique

Affinity purification

Light chain type

kappa

Concentration
Loading...
Purification notes

ab110319 was produced in vitro using hybridomas grown in serum-free medium. Purity: >95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319), expandable thumbnail

    Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)

    All lanes: Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1 µg/mL

    All lanes: Human heart homogenate at 20 µg

    Predicted band size: 43 kDa

  • Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319), expandable thumbnail

    Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-Vinculin antibody [EPR8185] ab129002 was used as a loading control.

    All lanes: Western blot - Anti-Creatine Kinase MM antibody [3E5BA5] (ab110319) at 1/50 dilution

    Lane 1: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Lane 3: Mouse placenta tissue lysate at 20 µg

    Lane 4: Human skeletal muscle tissue lysate at 20 µg

    Lane 5: Human spleen tissue lysate at 20 µg

    Lane 6: Human placenta tissue lysate at 20 µg

    Predicted band size: 43 kDa

    Observed band size: 43 kDa

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com