AB151465

Anti-Creatine Kinase MM antibody

Name: Anti-Creatine Kinase MM antibody (ab151465) | Abcam
Brand: Abcam Limited
SKU: AB151465
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Creatine Kinase MM antibody. Suitable for IHC-P, WB, ICC/IF, IHC - Wmt, IHC and reacts with Mouse, Human, Dog, Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CKM aa 100 to C-terminus.

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine Kinase MM antibody (AB151465)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine Kinase MM antibody (AB151465)

Immunohistochemical analysis of paraffin embedded mouse heart tissue stained for Creatine kinase (muscle) protein at cytosol with ab151465 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Creatine Kinase MM antibody (ab151465) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

10% SDS PAGE

All lanes:

Western blot - Anti-Creatine Kinase MM antibody (ab151465) at 1/1000 dilution

All lanes:

HeLaS3 whole cell lysate at 30 µg

Predicted band size: 43 kDa

false

Supplier Data

Immunohistochemistry - Anti-Creatine Kinase MM antibody (AB151465)

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using ab151465 at 1/200 dilution.

IHC - Wholemount - Anti-Creatine Kinase MM antibody (AB151465)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Creatine Kinase MM antibody (AB151465)

ab151465 detects Ckma protein on 2 days-post-fertilization zebrafish embryo by whole mount immunohistochemical analysis at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Creatine Kinase MM antibody (AB151465)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Creatine Kinase MM antibody (AB151465)

Immunofluorescence analysis of MDCK cells fixed in 4% paraformaldehyde at RT for 15 min labelling Creatine kinase (muscle) protein at cytoplasm using ab151465 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Creatine Kinase MM antibody (ab151465) at 1/1000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Zebrafish, Dog

Applications

IHC - Wmt, IHC, WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CKM aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06732

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "guaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "predicted", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "predicted", "IHC-species-dilution-info": "", "IHC-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "", "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

See full target information CKM

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal science and biotechnology 16:31 PubMed40022146

2025

Effects of dietary supplementation of creatine on fetal development in gilts at d 60 and d 90 of gestation.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna N Lopez,Maddison A Olivarez,Claire Stenhouse,Robyn M Moses,Makenzie G Newton,Nirvay Sah,Heewon Seo,Joseph Cain,Carli Lefevre,Alexandria Ross,Patrick Ryan,Jeffrey G Wiegert,Guoyao Wu,Gregory A Johnson,Fuller W Bazer

iScience 25:103680 PubMed35036870

2022

An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Juan R Alvarez-Dominguez,Sally Winther,Jacob B Hansen,Harvey F Lodish,Marko Knoll

International journal of molecular sciences 22: PubMed34502484

2021

Slow Off-Rate Modified Aptamer (SOMAmer) Proteomic Analysis of Patient-Derived Malignant Glioma Identifies Distinct Cellular Proteomes.

Applications

Unspecified application

Species

Unspecified reactive species

Thatchawan Thanasupawat,Aleksandra Glogowska,Christopher Pascoe,Sai Nivedita Krishnan,Maliha Munir,Farhana Begum,Jason Beiko,Jerry Krcek,Marc R Del Bigio,Marshall Pitz,Yaoqing Shen,Victor Spicer,Kevin M Coombs,John Wilkins,Sabine Hombach-Klonisch,Thomas Klonisch

International journal of biological macromolecules 162:11-23 PubMed32531365

2020

Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis.

Applications

Unspecified application

Species

Unspecified reactive species

Caiyan Li,Wei Wang,Jinhyuk Lee,Lifang Zeng,Yufei Yang,Shang-Jun Yin,Yong-Doo Park,Guo-Ying Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Creatine Kinase MM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine Kinase MM antibody (AB151465)

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

Immunohistochemistry - Anti-Creatine Kinase MM antibody (AB151465)

IHC - Wholemount - Anti-Creatine Kinase MM antibody (AB151465)

Immunocytochemistry/ Immunofluorescence - Anti-Creatine Kinase MM antibody (AB151465)

Western blot - Anti-Creatine Kinase MM antibody (AB151465)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise