JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174672

Anti-Creatine Kinase MM antibody

5

(1 Review)

|

(3 Publications)

Goat Polyclonal Creatine Kinase MM antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CKM aa 250-300.

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

1 Images
Western blot - Anti-Creatine Kinase MM antibody (AB174672)
  • WB

Supplier Data

Western blot - Anti-Creatine Kinase MM antibody (AB174672)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Creatine Kinase MM antibody (ab174672) at 0.03 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 43 kDa

Observed band size: 45 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CKM aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P06732

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab174672 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information CKM

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 12:773995 PubMed34975527

2021

Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Carlton D Fox,Paulo H C Mesquita,Joshua S Godwin,Vitor Angleri,Felipe Damas,Bradley A Ruple,Casey L Sexton,Michael D Brown,Andreas N Kavazis,Kaelin C Young,Carlos Ugrinowitsch,Cleiton A Libardi,Michael D Roberts

Journal of molecular and cellular cardiology 138:304-317 PubMed31836543

2019

Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Cole R Michel,Ying H Lin,Timothy A McKinsey,Mark Y Jeong,Amrut V Ambardekar,Joseph C Cleveland,Richard Reisdorph,Nichole Reisdorph,Kathleen C Woulfe,Kristofer S Fritz

PloS one 13:e0206801 PubMed30395621

2018

Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey M Lynch,Andrew J Dolman,Chenying Guo,Katie Dolan,Chuanxi Xiang,Samir Reda,Bing Li,Ganesh Prasanna
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com