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Rabbit Polyclonal Creatine kinase MT phospho Y153 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CKMT1A phospho Y153.

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Images

Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (AB228517), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.24% HEPES, 0.01% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CKMT1A phospho Y153. The exact immunogen used to generate this antibody is proprietary information. Database link P12532

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Mouse
Tested
Rat
Predicted
Cow
Predicted
Dog
Predicted
Guinea pig
Predicted
Hamster
Predicted
Rabbit
Predicted
Sheep
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Alternative names

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Rabbit Polyclonal Creatine kinase MT phospho Y153 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CKMT1A phospho Y153.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CKMT1A phospho Y153. The exact immunogen used to generate this antibody is proprietary information. Database link P12532
Purification technique

Affinity purification Immunogen

Specificity

Specific for endogenous levels of the ~46 kDa Creatine Kinase MT protein phosphorylated at Tyr153. Immunolabeling of Creatine Kinase MT is greatly decreased by lambda phosphatase treatment.

Concentration
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Purification notes

ab228517 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (ab228517), expandable thumbnail

    Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (ab228517)

    Phosphospecificity is shown in Lane 2 where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (1200 units for 30 minutes).

    All lanes: Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (ab228517) at 1/1000 dilution

    Lane 1: Mouse mitochondria from whole brain lysate

    Lane 2: Mouse mitochondria from whole brain lysate treated with lambda phosphatase (1200 units for 30 minutes)

    Predicted band size: 47 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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