AB221216

Anti-CREB antibody [E306] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(12 Publications)

Rabbit Recombinant Monoclonal CREB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

ab32515, at a 1/500 dilution, staining CREB in paraffin embedded human prostatic carcinoma tissue sections by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32515).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

This data was developed using ab32515, the same antibody clone in a different buffer formulation

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (ab221216) at 0.03 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

This data was developed using ab32515, the same antibody clone in a different buffer formulation :

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CREB knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : A431 cell lysate(20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32515 observed at 44 kDa. Red - loading control, ab18058, observed at 214 kDa.

ab32515 was shown to recognize CREB in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CREB knockout samples examined. Wild-type and knockout samples were subjected to SDS-PAGE. ab32515 and ab18058 (loading control to Vinculin) diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (ab221216)

Predicted band size: 36 kDa

false

Unconjugated

Anti-CREB antibody [E306]
HRP

HRP Anti-CREB antibody [E306]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E306

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes CREB, and can also recognize the splice isoform of CREB.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab221216 is the carrier-free version of ab32515.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.

Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).

See full target information CREB1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:1455-1463 PubMed27588067

2016

Effects of dihydrotestosterone on synaptic plasticity of the hippocampus in mild cognitive impairment male SAMP8 mice.

Applications

IHC-P

Species

Mouse

Wensen Pan,Shuo Han,Lin Kang,Sha Li,Juan Du,Huixian Cui

Frontiers in molecular neuroscience 8:70 PubMed26635523

2015

Morris Water Maze Training in Mice Elevates Hippocampal Levels of Transcription Factors Nuclear Factor (Erythroid-derived 2)-like 2 and Nuclear Factor Kappa B p65.

Applications

Species

Mouse

Wanda M Snow,Payam S Pahlavan,Jelena Djordjevic,Danielle McAllister,Eric E Platt,Shoug Alashmali,Michael J Bernstein,Miyoung Suh,Benedict C Albensi

Hepatology (Baltimore, Md.) 58:2099-108 PubMed23744808

2013

Receptor interacting protein kinase 3 is a critical early mediator of acetaminophen-induced hepatocyte necrosis in mice.

Applications

Species

Unspecified reactive species

Anup Ramachandran,Mitchell R McGill,Yuchao Xie,Hong-Min Ni,Wen-Xing Ding,Hartmut Jaeschke

Hepatology (Baltimore, Md.) 58:1011-20 PubMed23532963

2013

Mutual interaction between YAP and CREB promotes tumorigenesis in liver cancer.

Applications

Unspecified application

Species

Human

Jiayi Wang,Lifang Ma,Wenhao Weng,Yongxia Qiao,Yue Zhang,Jiangtu He,Hongmei Wang,Weifan Xiao,Lanlan Li,Qinghua Chu,Qiuhui Pan,Yongchun Yu,Fenyong Sun

Neurochemistry international 63:283-90 PubMed23831214

2013

Protective effects of EphB2 on Aβ1-42 oligomer-induced neurotoxicity and synaptic NMDA receptor signaling in hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Geng,Lin Kang,Yuhong Su,Jianxin Jia,Jun Ma,Sha Li,Juan Du,Huixian Cui

PloS one 8:e54261 PubMed23349840

2013

Cyclic AMP enhances TGFβ responses of breast cancer cells by upregulating TGFβ receptor I expression.

Applications

Species

Human

Ilka Oerlecke,Elke Bauer,Angela Dittmer,Benjamin Leyh,Jürgen Dittmer

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26:778-87 PubMed22049059

2011

Phthalates induce proliferation and invasiveness of estrogen receptor-negative breast cancer through the AhR/HDAC6/c-Myc signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Hua Hsieh,Cheng-Fang Tsai,Chia-Yi Hsu,Po-Lin Kuo,Jau-Nan Lee,Chee-Yin Chai,Shao-Chun Wang,Eing-Mei Tsai

Brain research 1417:127-36 PubMed21907331

2011

Caffeine induces beneficial changes in PKA signaling and JNK and ERK activities in the striatum and cortex of Alzheimer's transgenic mice.

Applications

Unspecified application

Species

Mouse

Ross Zeitlin,Sagar Patel,Sarah Burgess,Gary W Arendash,Valentina Echeverria

Cancer research 71:4720-31 PubMed21555366

2011

Akt2 regulates all Akt isoforms and promotes resistance to hypoxia through induction of miR-21 upon oxygen deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Christos Polytarchou,Dimitrios Iliopoulos,Maria Hatziapostolou,Filippos Kottakis,Ioanna Maroulakou,Kevin Struhl,Philip N Tsichlis

The EMBO journal 29:469-81 PubMed19942859

2009

PKA phosphorylates and inactivates AMPKalpha to promote efficient lipolysis.

Applications

Species

Mouse

Nabil Djouder,Roland D Tuerk,Marianne Suter,Paolo Salvioni,Ramon F Thali,Roland Scholz,Kari Vaahtomeri,Yolanda Auchli,Helene Rechsteiner,René A Brunisholz,Benoit Viollet,Tomi P Mäkelä,Theo Wallimann,Dietbert Neumann,Wilhelm Krek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CREB antibody [E306] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

Western blot - Anti-CREB antibody [E306] - BSA and Azide free (AB221216)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise