Rabbit Polyclonal CREB phospho S129 + S133 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CREB1 phospho S129.
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- Experimental and therapeutic medicine 22:14422021Repeated exposure to sevoflurane in neonatal rats impairs cognition in adulthood via the PKA-CREB-BDNF signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJili Zhao,Jinyu Ren,Shuang Liu,Yanan Gong,Ping Meng,Haitao Tan,Yonggang ChenPubMed 34721684
- The Journal of physiology 596:4121-41392018Beta -adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorten Hostrup,Søren Reitelseder,Søren Jessen,Anders Kalsen,Michael Nyberg,Jon Egelund,Michael Kreiberg,Caroline Maag Kristensen,Martin Thomassen,Henriette Pilegaard,Vibeke Backer,Glenn A Jacobson,Lars Holm,Jens BangsboPubMed 29968301
- Molecular medicine reports 17:8530-85352018Norepinephrine inhibits the cytotoxicity of NK92‑MI cells via the β2‑adrenoceptor/cAMP/PKA/p‑CREB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZan Sun et. al.PubMed 29658580
- Journal of autoimmunity 90:49-582018TGF-β signalling defect is linked to low CD39 expression on regulatory T cells and methotrexate resistance in rheumatoid arthritis.
- Journal of neurochemistry 146:133-1442018Genetic removal of eIF2α kinase PERK in mice enables hippocampal L-LTP independent of mTORC1 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelena R Zimmermann,Wenzhong Yang,Brenna C Beckelman,Nicole P Kasica,Xueyan Zhou,Lucas Dufresne Galli,Alexey G Ryazanov,Tao MaPubMed 29337352
- Cancer immunology, immunotherapy : CII 60:111-222010Receptor desensitization and blockade of the suppressive effects of prostaglandin E(2) and adenosine on the cytotoxic activity of human melanoma-infiltrating T lymphocytes.
- Cell communication and signaling : CCS 3:102005Beta-adrenergic receptor activation in immortalized human urothelial cells stimulates inflammatory responses by PKA-independent mechanisms.Applications:WBReactive species:Unspecified reactive speciesErin B Harmon,Jill M Porter,James E PorterPubMed 16091148
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CREB1 phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P16220
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Alternative names
Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Recommended products
Rabbit Polyclonal CREB phospho S129 + S133 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CREB1 phospho S129.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CREB1 phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P16220
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a spanning peptide corresponding to the cleavage-site to remove antibody that is reactive with non-phosphorylated CREB. The final product is generated by affinity chromatography using a CREB-derived peptide that is phosphorylated at serines 129 and 133.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
- Biological function summary
CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.
- Pathways
CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.
- Associated diseases and disorders
Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564)
The membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method.
All lanes: Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564) at 1/1000 dilution
Lane 1: NIH3T3 cell extract
Lane 2: NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract
Lane 3: NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4: NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with a generic phosphoserine-containing peptide
Lane 5: NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, incubated with the phosphopeptide immunogen
Lane 6: NIH3T3 treated with 50 ng/mL PDGF for 15 minutes cell extract, treated with lambda phosphatase
Predicted band size: 36 kDa
Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564)
All lanes: Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564) at 0.5 µg/mL
Lane 1: Human A549 whole cell extract at 20000 Cells
Lane 2: Human A549 whole cell extract stimulated with EGF at 20000 Cells
Lane 3: Human A549 whole cell extract treated with pervanadate at 20000 Cells
SecondaryAll lanes: Anti-mouse, HRP-conjugated
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 30s
Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564)
Detection: Chemiluminescence.All lanes: Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564) at 1/1000 dilution
Lane 1: NIH/3T3 cell lysate at 20 µg
Lane 2: NIH/3T3 treated for 10 minutes with 50 ng/mL of PDGF cell lysate at 20 µg
Lane 3: NIH/3T3 treated for 10 minutes with 200 ng/mL of EGF cell lysate at 20 µg
Lane 4: A549 cell lysate at 20 µg
Lane 5: A549 treated for 10 minutes with 200 ng/mL of EGF cell lysate at 20 µg
Lane 6: A431 cell lysate at 20 µg
Lane 7: SK-N-SH cell lysate at 20 µg
Lane 8: MCF7 cell lysate at 20 µg
Lane 9: MDA-MB-231 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 35 kDa, 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S129 + S133) antibody (ab10564)
Immunofluorescent analysis of CREB (phospho S129/pS133) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with CREB (phospho S129/pS133) Rabbit polyclonal Antibody (ab10564) at 2 μg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CREB (phospho S129 + S133) antibody (ab10564)
CREB (phospho S129 + S133) western blot using anti-CREB (phospho S129 + S133) antibody ab10564. Publication image and figure legend from Harmon, E. B., Porter, J. M., et al., 2005, Cell Commun Signal, PubMed 16091148.
ab10564 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab10564 please see the product overview.
PKA inhibitors H-89 and Rp-cAMPS block phosphorylation of CREB. After a 30 min pre-incubation with serum-free DMEM alone or with 100 nM of H-89 or 10 μM of Rp-cAMPS, UROtsa cells were stimulated with 100 nM isoproterenol in serum-free DMEM for the indicated times and immunoblotted with an antibody specific to CREB phosphorylated at S129 and S133 (pCREB). Without inhibitor pretreatment, isoproterenol induced CREB phosphorylation within 5 min. Significant phosphorylation was still observed 15 min. after drug addition with banding intensity patterns similar to basal after 60 min. Preincubation with H-89 or Rp-cAMPS significantly decreased the phosphorylation of CREB at the 5 and 15 min time points in UROtsa cells, demonstrating the effectiveness of PKA inhibition for these compounds. As reported by the antibody suppliers, pCREB is identified as a band running at 43 kD. The second band seen on the autoradiographs represents a previously-reported alternative splice variant of CREB. Autoradiographs are representative immunoblots of n = 3 independent cell treatments.
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