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AB194687

Anti-CREB (phospho S133) antibody

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(4 Publications)

Rabbit Polyclonal CREB phospho S133 + S133 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CREB1 phospho S133.

View Alternative Names

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody (AB194687)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody (AB194687)

Immunofluorescent analysis of MCF cells labeling CREB (phospho S133) with ab194687 at 1/50 dilution. DAPI staining of the nuclei is blue.

Western blot - Anti-CREB (phospho S133) antibody (AB194687)
  • WB

Supplier Data

Western blot - Anti-CREB (phospho S133) antibody (AB194687)

All lanes:

Western blot - Anti-CREB (phospho S133) antibody (ab194687) at 1/500 dilution

Lane 1:

Untreated HeLa cell extracts

Lane 2:

HeLa cell extracts treated with UV

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CREB1 phospho S133. The exact immunogen used to generate this antibody is proprietary information.

P16220

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
See full target information CREB phospho S133 + S133
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:21 PubMed39849382

2025

AdipoR1 enhances the radiation resistance via ESR1/CCNB1IP1/cyclin B1 pathway in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Gan,Linhui Zhu,Yimo Li,Ruoting Ge,Jiahe Tian,Yuxin Chen,Xiang He,Shumei Ma,Xiaodong Liu

Scientific reports 11:21766 PubMed34741086

2021

PER2 mediates CREB-dependent light induction of the clock gene Per1.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Brenna,Jürgen A Ripperger,Gabriella Saro,Dominique A Glauser,Zhihong Yang,Urs Albrecht

Scientific reports 10:16714 PubMed33028897

2020

Acrylamide alters CREB and retinoic acid signalling pathways during differentiation of the human neuroblastoma SH-SY5Y cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Attoff,Ylva Johansson,Andrea Cediel-Ulloa,Jessica Lundqvist,Rajinder Gupta,Florian Caiment,Anda Gliga,Anna Forsby

Cell 179:1160-1176.e24 PubMed31730855

2019

Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Huang,Zhanghua Chen,Lanlan Geng,Jun Wang,Huiying Liang,Yujie Cao,Huan Chen,Wanming Huang,Meiling Su,Hanqing Wang,Yanhui Xu,Yukun Liu,Bingtai Lu,Huifang Xian,Huiwen Li,Huilin Li,Lu Ren,Jing Xie,Liping Ye,Hongli Wang,Junhong Zhao,Peiyu Chen,Li Zhang,Shanmeizi Zhao,Ting Zhang,Banglao Xu,Di Che,Wenyue Si,Xiaoqiong Gu,Liang Zeng,Yong Wang,Dingyou Li,Yifan Zhan,David Delfouneso,Andrew M Lew,Jun Cui,Wai Ho Tang,Yan Zhang,Sitang Gong,Fan Bai,Min Yang,Yuxia Zhang
View all publications
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