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AB254107

Anti-CREB (phospho S133) antibody

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(6 Publications)

Rabbit Polyclonal CREB phospho S133 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Rat Creb1 phospho S133.

View Alternative Names

Creb-1, Creb1, Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody (AB254107)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREB (phospho S133) antibody (AB254107)

Mouse piriform cortex tissue stained for CREB (phospho S133) using ab254107 in immunohistochemical analysis. the nuclear counterstain is DAPI (blue).

Western blot - Anti-CREB (phospho S133) antibody (AB254107)
  • WB

Supplier Data

Western blot - Anti-CREB (phospho S133) antibody (AB254107)

All lanes:

Western blot - Anti-CREB (phospho S133) antibody (ab254107) at 1/1000 dilution

Lane 1:

Rat hippocampus lysate

Lane 2:

Rat hippocampus lysate - treated with Lambda phosphatase (800 units / mg protein for 30 minutes)

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Creb1 phospho S133. The exact immunogen used to generate this antibody is proprietary information.

P15337

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (By similarity). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (PubMed : 30566056).
See full target information Creb1 phospho S133
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Human cell 38:96 PubMed40279007

2025

Deficiency of synaptotagmin-1 aggravates pressure overload-induced cardiac hypertrophy and dysfunction via the p38 MAPK signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shen,Junqiu Miao,Lifei Wu,Deping Wang,Guang Li,Haixiong Wang,Jimin Cao

Brain sciences 15: PubMed40149758

2025

The pCREB/BDNF Pathway in the Hippocampus Is Involved in the Therapeutic Effect of Selective 5-HT Reuptake Inhibitors in Adult Male Rats Exposed to Blast Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Fan,Hong Wang,Xiaoqiang Lv,Qi Wang,Boya Yu,Xiao Li,Liang Li,Yuhao Zhang,Ning Ma,Qing Lu,Airong Qian,Junhong Gao

Scientific reports 14:16260 PubMed39009622

2024

Pre-IVM with C-type natriuretic peptide promotes mitochondrial biogenesis of bovine oocytes via activation of CREB.

Applications

Unspecified application

Species

Unspecified reactive species

Zehua Zhang,Zhenwei Jia

Acta biochimica et biophysica Sinica 55:1275-1287 PubMed37365870

2023

Galangin mitigates glucocorticoid-induced osteoporosis by activating autophagy of BMSCs via triggering the PKA/CREB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenying Zeng,Shan Wang,Huimin Gu,Fenglei Chen,Ziming Wang,Jinteng Li,Zhongyu Xie,Pei Feng,Huiyong Shen,Yanfeng Wu

Journal of ovarian research 16:83 PubMed37106468

2023

BDNF promotes mouse follicular development and reverses ovarian aging by promoting cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Yongjie Liu,Shuman Li,Pingping Chen,Jun Zhang,Liping Feng

EBioMedicine 85:104274 PubMed36182775

2022

Inhibition of HIPK2 protects stress-induced pathological cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiulian Zhou,Danni Meng,Feng Li,Xiao Zhang,Li Liu,Yujiao Zhu,Shuqin Liu,Minjun Xu,Jiali Deng,Zhiyong Lei,Joost P G Sluijter,Junjie Xiao
View all publications
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