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AB327195

Anti-CREB1 Antibody

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Rabbit Polyclonal CREB antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human CREB1.

View Alternative Names

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CREB1 Antibody (AB327195)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREB1 Antibody (AB327195)

Immunofluorescent staining of human cell line U-2 OS using ab327195 at 0.25–2 µg/ml, showing localization to nucleoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB1 Antibody (AB327195)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB1 Antibody (AB327195)

Immunohistochemical staining of human small intestine using ab327195 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in glandular cells.

ChIP - Anti-CREB1 Antibody (AB327195)
  • ChIP

Supplier Data

ChIP - Anti-CREB1 Antibody (AB327195)

ChIP-Exo-Seq composite graph for ab327195 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-CREB1 Antibody (AB327195)
  • WB

Supplier Data

Western blot - Anti-CREB1 Antibody (AB327195)

Western blot analysis using ab327195 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : Human cell line RT-4
Lane 3 : Human cell line U-251MG sp

All lanes:

Western blot - Anti-CREB1 Antibody (ab327195)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251MG sp

Predicted band size: 35.14 kDa

false

Western blot - Anti-CREB1 Antibody (AB327195)
  • WB

Supplier Data

Western blot - Anti-CREB1 Antibody (AB327195)

Western blot analysis using ab327195 at 0.04–0.4 µg/ml.
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)

All lanes:

Western blot - Anti-CREB1 Antibody (ab327195)

Lane 1:

NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)

Lane 2:

NBT-II cell lysate (Rat Wistar bladder tumour cells)

Predicted band size: 35.14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ChIP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CREB1.

P16220

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
See full target information CREB1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com