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AB277098

Anti-CREB5 antibody [PCRP-CREB5-1G8]

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(1 Publication)

Mouse Monoclonal CREB5 antibody. Suitable for ICC/IF, Flow Cyt, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CREB5.

View Alternative Names

CREBPA, CREB5, Cyclic AMP-responsive element-binding protein 5, CREB-5, cAMP-responsive element-binding protein 5, cAMP-response element-binding protein A, CRE-BPa

5 Images
Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 using ab277098 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/ Immunofluorescence - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 with ab277098 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CREB5 using ab277098 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)
  • WB

Supplier Data

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

All lanes:

Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] (ab277098) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 56 kDa

false

Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)
  • Protein Array

Supplier Data

Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] (AB277098)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277098.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-CREB5-1G8

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, Protein Array, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CREB5.

Q02930

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CREB5 also known as cAMP responsive element binding protein 5 is a transcription factor important for regulating gene expression. This protein has a mass of approximately 57 kDa and belongs to the CREB family of proteins. It is found expressed in various tissues including the brain lung and kidney. CREB5 binds to the cAMP response element (CRE) sequence found in the promoters of certain genes to modulate their transcription.
Biological function summary

CREB5 plays a role in the response to cellular stress and adaptation. It participates in mediating cellular responses through transcriptional activation influenced by signals such as hormones growth factors and neurotransmitters. CREB5 forms complexes with other transcription factors and coactivators enhancing or repressing the transcription of target genes depending on the context. It influences processes such as differentiation proliferation and survival of cells.

Pathways

CREB5 is involved in the cAMP signaling pathways and MAPK/ERK pathways. These pathways regulate various cellular functions including metabolism cell cycle progression and synaptic plasticity. CREB5 interacts with proteins like CREB1 and CREB3 which together contribute to the dysregulation of these pathways when mutations or malfunctions occur.

CREB5 has been associated with neurodegenerative disorders like Alzheimer's disease and certain types of cancer such as lung cancer. Variants in the expression or function of CREB5 can markedly affect the disease process often interacting with proteins like ATF4 in Alzheimer's which is implicated in stress responses and with NF-kB in cancer involved in immune response and cell survival mechanisms. Understanding CREB5's role provides insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the cAMP response element and activates transcription.
See full target information CREB5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 11:1186-1199 PubMed33456992

2021

CircBACH1/let-7a-5p axis enhances the proliferation and metastasis of colorectal cancer by upregulating CREB5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jutang Li,Qian Tang,Wei Dong,Yizhou Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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