Rabbit Polyclonal CREBBP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CREBBP.
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- Cell reports 38:1105462022ADRAM is an experience-dependent long noncoding RNA that drives fear extinction through a direct interaction with the chaperone protein 14-3-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wei,Qiongyi Zhao,Ziqi Wang,Wei-Siang Liau,Dean Basic,Haobin Ren,Paul R Marshall,Esmi L Zajaczkowski,Laura J Leighton,Sachithrani U Madugalle,Mason Musgrove,Ambika Periyakaruppiah,Jichun Shi,Jianjian Zhang,John S Mattick,Timothy R Mercer,Robert C Spitale,Xiang Li,Timothy W BredyPubMed 35320727
- Cell & bioscience 5:172015Division of labor between IRF1 and IRF2 in regulating different stages of transcriptional activation in cellular antiviral activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Ren et. al.PubMed 25960866
- Endocrinology 155:3674-832014Histone deacetylase inhibition induces long-lasting changes in maternal behavior and gene expression in female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle S Stolzenberg,Jacqueline S Stevens,Emilie F RissmanPubMed 24932804
- The Journal of experimental medicine 208:1809-222011High-level IGF1R expression is required for leukemia-initiating cell activity in T-ALL and is supported by Notch signaling.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CREBBP. The exact immunogen used to generate this antibody is proprietary information. Database link Q92793
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acetylates histones, giving a specific tag for transcriptional activation (PubMed:21131905, PubMed:24616510). Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively) (PubMed:21131905). Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1 (PubMed:10490106, PubMed:11154691, PubMed:12738767, PubMed:12929931, PubMed:24207024, PubMed:28790157, PubMed:30540930, PubMed:35675826, PubMed:9707565). Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates POLR1E/PAF53, leading to decreased association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:24207024). Acetylates DDX21, thereby inhibiting DDX21 helicase activity (PubMed:28790157). Acetylates FBL, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as lactoyl-CoA, and is able to mediate protein lactylation (PubMed:38128537). Catalyzes lactylation of MRE11 in response to DNA damage, thereby promoting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:38128537). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed:25514493).
Alternative names
CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein lactyltransferas CREBBP, Protein-lysine acetyltransferase CREBBP
Recommended products
Rabbit Polyclonal CREBBP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CREBBP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CREBBP. The exact immunogen used to generate this antibody is proprietary information. Database link Q92793
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CREBBP also known as CREB Binding Protein or CBP is a multifunctional protein with a molecular weight of approximately 265 kDa. This protein serves as a transcriptional coactivator and possesses intrinsic histone acetyltransferase (HAT) activity. CREBBP is ubiquitously expressed making it present in various tissues throughout the body. Its interaction with different transcription factors facilitates transcriptional activation by remodeling chromatin structure.
- Biological function summary
CREBBP plays a pivotal role in regulating gene expression by serving as a coactivator that bridges transcription factors with the basal transcription machinery. CREBBP as part of large multiprotein complexes acetylates histones as well as non-histone proteins influencing chromatin accessibility and transcriptional efficiency. Its HAT activity contributes to the regulation of cell cycle differentiation and DNA repair.
- Pathways
CREBBP integrates into both the p53 and Hedgehog signaling pathways modulating various cellular responses. In the p53 pathway CREBBP interacts with the tumor suppressor protein p53 enhancing p53’s ability to initiate expression of genes involved in cell cycle arrest and apoptosis. In the Hedgehog pathway CREBBP regulates transcription factors such as Gli proteins. The interplay between CREBBP and these pathways underlines its importance in maintaining cellular homeostasis and preventing oncogenesis.
- Associated diseases and disorders
Mutations or dysregulation of CREBBP are associated with Rubinstein-Taybi syndrome and various cancers including acute myeloid leukemia. In Rubinstein-Taybi syndrome CREBBP mutations impair cognitive development and skeletal malformations. In cancer particularly acute myeloid leukemia alterations in CREBBP disrupt normal cell growth regulation and lead to tumorigenesis. The protein's interaction with factors like p300 another histone acetyltransferase further emphasizes its critical involvement in these pathological conditions.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-CREBBP antibody (ab10489)
ab10489 immunoprecipitating CREBBP at 6 μg/ml lysate.
Lane 1: Anti-CREBBP antibody ab10489 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 2: Anti-CREBBP antibody Anti-CREBBP antibody ab10490 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).
Lane 3: IgG control.
For blotting immunoprecipitated CREBBP, ab10489 was used at 1 μg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1: Immunoprecipitation - Anti-CREBBP antibody (ab10489)
Lane 1: Immunoprecipitation - Anti-CREBBP antibody (Anti-CREBBP antibody ab10490)
Predicted band size: 265 kDa
Western blot - Anti-CREBBP antibody (ab10489)
All lanes: Western blot - Anti-CREBBP antibody (ab10489) at 0.4 µg/mL
Lane 1: HeLa cell lysate at 50 µg
Lane 2: HeLa cell lysate at 15 µg
Lane 3: HeLa cell lysate at 5 µg
Predicted band size: 265 kDa
Exposure time: 3min
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