AB235270

Anti-CREBBP antibody

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(2 Publications)

Rabbit Polyclonal CREBBP antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein lactyltransferas CREBBP, Protein-lysine acetyltransferase CREBBP

2 Images

Supplier Data

Western blot - Anti-CREBBP antibody (AB235270)

All lanes:

Western blot - Anti-CREBBP antibody (ab235270) at 5 µg/mL

Lane 1:

MOLT-4 (Human lymphoblastic luekemia cell line) whole cell lysate at 35 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 35 µg

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 4:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 5:

Mouse brain whole tissue lysate at 35 µg

Secondary

All lanes:

Goat anti-Rabbit [H&L] HRP at 1/70000 dilution

Predicted band size: 265 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-CREBBP antibody (AB235270)

7.5% gel.

All lanes:

Western blot - Anti-CREBBP antibody (ab235270) at 5 µg/mL

Lane 1:

Mouse brain whole tissue lysate at 35 µg

Lane 2:

Human eye tissue lysate at 35 µg

Secondary

All lanes:

Goat anti-Rabbit IgG [H&L] HRP at 1/70000 dilution

Predicted band size: 265 kDa

false

Exposure time: 60s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CREBBP also known as CREB Binding Protein or CBP is a multifunctional protein with a molecular weight of approximately 265 kDa. This protein serves as a transcriptional coactivator and possesses intrinsic histone acetyltransferase (HAT) activity. CREBBP is ubiquitously expressed making it present in various tissues throughout the body. Its interaction with different transcription factors facilitates transcriptional activation by remodeling chromatin structure.

Biological function summary

CREBBP plays a pivotal role in regulating gene expression by serving as a coactivator that bridges transcription factors with the basal transcription machinery. CREBBP as part of large multiprotein complexes acetylates histones as well as non-histone proteins influencing chromatin accessibility and transcriptional efficiency. Its HAT activity contributes to the regulation of cell cycle differentiation and DNA repair.

Pathways

CREBBP integrates into both the p53 and Hedgehog signaling pathways modulating various cellular responses. In the p53 pathway CREBBP interacts with the tumor suppressor protein p53 enhancing p53’s ability to initiate expression of genes involved in cell cycle arrest and apoptosis. In the Hedgehog pathway CREBBP regulates transcription factors such as Gli proteins. The interplay between CREBBP and these pathways underlines its importance in maintaining cellular homeostasis and preventing oncogenesis.

Mutations or dysregulation of CREBBP are associated with Rubinstein-Taybi syndrome and various cancers including acute myeloid leukemia. In Rubinstein-Taybi syndrome CREBBP mutations impair cognitive development and skeletal malformations. In cancer particularly acute myeloid leukemia alterations in CREBBP disrupt normal cell growth regulation and lead to tumorigenesis. The protein's interaction with factors like p300 another histone acetyltransferase further emphasizes its critical involvement in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acetylates histones, giving a specific tag for transcriptional activation (PubMed : 21131905, PubMed : 24616510). Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively) (PubMed : 21131905). Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1 (PubMed : 10490106, PubMed : 11154691, PubMed : 12738767, PubMed : 12929931, PubMed : 24207024, PubMed : 28790157, PubMed : 30540930, PubMed : 35675826, PubMed : 9707565). Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators : NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed : 14645221). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed : 24939902). Acetylates POLR1E/PAF53, leading to decreased association of RNA polymerase I with the rDNA promoter region and coding region (PubMed : 24207024). Acetylates DDX21, thereby inhibiting DDX21 helicase activity (PubMed : 28790157). Acetylates FBL, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed : 30540930). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as lactoyl-CoA, and is able to mediate protein lactylation (PubMed : 38128537). Catalyzes lactylation of MRE11 in response to DNA damage, thereby promoting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 38128537). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed : 25514493).

See full target information CREBBP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401939 PubMed38704700

2024

The Long Non-Coding RNA Obesity-Related (Obr) Contributes To Lipid Metabolism Through Epigenetic Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Suneesh Kaimala,Shareena Saeed Lootah,Neha Mehra,Challagandla Anil Kumar,Saeeda Al Marzooqi,Prabha Sampath,Suraiya Anjum Ansari,Bright Starling Emerald

PLoS biology 21:e3002386 PubMed37983249

2023

Brn3b regulates the formation of fear-related midbrain circuits and defensive responses to visual threat.

Applications

Unspecified application

Species

Unspecified reactive species

Hyoseo Lee,Hannah Weinberg-Wolf,Hae-Lim Lee,Tracy Lee,Joseph Conte,Carlos Godoy-Parejo,Jonathan B Demb,Andrii Rudenko,In-Jung Kim

View all publications

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Product promise

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Anti-CREBBP antibody

Western blot - Anti-CREBBP antibody (AB235270)

Western blot - Anti-CREBBP antibody (AB235270)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise