Rabbit Polyclonal CREBBP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 58 publications. Immunogen corresponding to Synthetic Peptide within Human CREBBP aa 150-200.
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- Cellular and molecular gastroenterology and hepatology 14:1177-11982022cccDNA Surrogate MC-HBV-Based Screen Identifies Cohesin Complex as a Novel HBV Restriction Factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuanchang Wu,Liyuan Wang,Xin Wang,Yang Sun,Haoran Li,Zhaoying Zhang,Caiyue Ren,Xiaohui Zhang,Shuangjie Li,Jinghui Lu,Leiqi Xu,Xuetian Yue,Yue Hong,Qiang Li,Haizhen Zhu,Yaoqin Gong,Chengjiang Gao,Huili Hu,Lifen Gao,Xiaohong Liang,Chunhong MaPubMed 35987451
- Oxidative medicine and cellular longevity 2022:88257842022BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP Delivery of miR-877-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDapeng Feng,Zhengwei Li,Liang Yang,Haidong Liang,Hongtao He,Lin Liu,Wei ZhangPubMed 35281474
- Heliyon 8:e088902022Involvement of activator protein-1 family members in β-catenin and p300 association on the genome of PANC-1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomomitsu Doi,Hironori Hojo,Shinsuke Ohba,Kunie Obayashi,Motoyoshi Endo,Toshimasa Ishizaki,Akira Katoh,Hiroyuki KoujiPubMed 35198763
- Nature communications 12:60602021Inhibition of CBP synergizes with the RNA-dependent mechanisms of Azacitidine by limiting protein synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeannine Diesch,Marguerite-Marie Le Pannérer,René Winkler,Raquel Casquero,Matthias Muhar,Mark van der Garde,Michael Maher,Carolina Martínez Herráez,Joan J Bech-Serra,Michaela Fellner,Philipp Rathert,Nigel Brooks,Lurdes Zamora,Antonio Gentilella,Carolina de la Torre,Johannes Zuber,Katharina S Götze,Marcus BuschbeckPubMed 34663789
- Cell death & disease 12:6852021ZFP36 protects lungs from intestinal I/R-induced injury and fibrosis through the CREBBP/p53/p21/Bax pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongmei Cao,Weifeng Huang,Fang Wu,Jiawei Shang,Feng Ping,Wei Wang,Yingchuan Li,Xuan Zhao,Xiaoping ZhangPubMed 34238924
- mBio 12:2021Multiple Viral microRNAs Regulate Interferon Release and Signaling Early during Infection with Epstein-Barr Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMickaël Bouvet,Stefanie Voigt,Takanobu Tagawa,Manuel Albanese,Yen-Fu Adam Chen,Yan Chen,Devin N Fachko,Dagmar Pich,Christine Göbel,Rebecca L Skalsky,Wolfgang HammerschmidtPubMed 33785626
- Frontiers in immunology 11:6255422021CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowen Bi,Baolin Jiang,Jinyi Zhou,Xirui Fan,Xintong Yan,Juanjuan Liang,Lan Luo,Zhimin YinPubMed 33603756
- Signal transduction and targeted therapy 6:102021CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao-Hui Huang et. al.PubMed 33431788
- Molecular medicine reports 22:2351-23632020Upregulated microRNA‑330‑3p promotes calcification in the bicuspid aortic valve via targeting CREBBP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zheng et. al.PubMed 32705274
- Biomaterials 246:1200002020Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonhwa Lee et. al.PubMed 32247936
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CREBBP aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q92793
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested |
| Primates | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acetylates histones, giving a specific tag for transcriptional activation (PubMed:21131905, PubMed:24616510). Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively) (PubMed:21131905). Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1 (PubMed:10490106, PubMed:11154691, PubMed:12738767, PubMed:12929931, PubMed:24207024, PubMed:28790157, PubMed:30540930, PubMed:35675826, PubMed:9707565). Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates POLR1E/PAF53, leading to decreased association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:24207024). Acetylates DDX21, thereby inhibiting DDX21 helicase activity (PubMed:28790157). Acetylates FBL, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as lactoyl-CoA, and is able to mediate protein lactylation (PubMed:38128537). Catalyzes lactylation of MRE11 in response to DNA damage, thereby promoting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:38128537). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed:25514493).
Alternative names
CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein lactyltransferas CREBBP, Protein-lysine acetyltransferase CREBBP
Recommended products
Rabbit Polyclonal CREBBP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 58 publications. Immunogen corresponding to Synthetic Peptide within Human CREBBP aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CREBBP aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q92793
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CREBBP also known as CREB Binding Protein or CBP is a multifunctional protein with a molecular weight of approximately 265 kDa. This protein serves as a transcriptional coactivator and possesses intrinsic histone acetyltransferase (HAT) activity. CREBBP is ubiquitously expressed making it present in various tissues throughout the body. Its interaction with different transcription factors facilitates transcriptional activation by remodeling chromatin structure.
- Biological function summary
CREBBP plays a pivotal role in regulating gene expression by serving as a coactivator that bridges transcription factors with the basal transcription machinery. CREBBP as part of large multiprotein complexes acetylates histones as well as non-histone proteins influencing chromatin accessibility and transcriptional efficiency. Its HAT activity contributes to the regulation of cell cycle differentiation and DNA repair.
- Pathways
CREBBP integrates into both the p53 and Hedgehog signaling pathways modulating various cellular responses. In the p53 pathway CREBBP interacts with the tumor suppressor protein p53 enhancing p53’s ability to initiate expression of genes involved in cell cycle arrest and apoptosis. In the Hedgehog pathway CREBBP regulates transcription factors such as Gli proteins. The interplay between CREBBP and these pathways underlines its importance in maintaining cellular homeostasis and preventing oncogenesis.
- Associated diseases and disorders
Mutations or dysregulation of CREBBP are associated with Rubinstein-Taybi syndrome and various cancers including acute myeloid leukemia. In Rubinstein-Taybi syndrome CREBBP mutations impair cognitive development and skeletal malformations. In cancer particularly acute myeloid leukemia alterations in CREBBP disrupt normal cell growth regulation and lead to tumorigenesis. The protein's interaction with factors like p300 another histone acetyltransferase further emphasizes its critical involvement in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of MCF-7 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2832, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of HeLa cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody (ab2832)
Immunohistochemical analysis of FFPE mouse colon tissue, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Heat antigen retrieval performed with 10mM Sodium Citrate (pH 6) for 8-15 minutes. Tissue blocked with 3% H2O2-methanol for 15 minutes at room temperature. Incubation with ab2832 diluted 1/2000 in 3% BSA-PBS overnight at 4°C. Counterstaining with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody (ab2832)
Immunofluorescent analysis of NIH-3T3 cells, labeling CREBBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Nuclei were stained with DAPI (blue).
Western blot - Anti-CREBBP antibody (ab2832)
ab2832 using HeLa cell lysate. ab2832 using HeLa cell lysate.
All lanes: Western blot - Anti-CREBBP antibody (ab2832)
Predicted band size: 265 kDa
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