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Mouse Monoclonal CREBBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CREBBP.


Images

Western blot - Anti-CREBBP antibody [CBP5I001] (AB50702), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (AB50702), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody [CBP5I001] (AB50702), expandable thumbnail
  • Western blot - Anti-CREBBP antibody [CBP5I001] (AB50702), expandable thumbnail
  • Western blot - Anti-CREBBP antibody [CBP5I001] (AB50702), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CREBBP. Database link Q92793

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50.00000 - 1/100.00000
Notes

-

Species
Human
Dilution info
1/50.00000 - 1/100.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Acetylates histones, giving a specific tag for transcriptional activation (PubMed:21131905, PubMed:24616510). Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively) (PubMed:21131905). Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1 (PubMed:10490106, PubMed:11154691, PubMed:12738767, PubMed:12929931, PubMed:24207024, PubMed:28790157, PubMed:30540930, PubMed:35675826, PubMed:9707565). Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates POLR1E/PAF53, leading to decreased association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:24207024). Acetylates DDX21, thereby inhibiting DDX21 helicase activity (PubMed:28790157). Acetylates FBL, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as lactoyl-CoA, and is able to mediate protein lactylation (PubMed:38128537). Catalyzes lactylation of MRE11 in response to DNA damage, thereby promoting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:38128537). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed:25514493).

Alternative names

CBP, CREBBP, CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein lactyltransferas CREBBP, Protein-lysine acetyltransferase CREBBP

Recommended products

Mouse Monoclonal CREBBP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CREBBP.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CREBBP. Database link Q92793
Clone number
CBP5I001
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted(approximately 95%) fetal bovine serum, and filtered through a 0.22 µm membrane.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CREBBP also known as CREB Binding Protein or CBP is a multifunctional protein with a molecular weight of approximately 265 kDa. This protein serves as a transcriptional coactivator and possesses intrinsic histone acetyltransferase (HAT) activity. CREBBP is ubiquitously expressed making it present in various tissues throughout the body. Its interaction with different transcription factors facilitates transcriptional activation by remodeling chromatin structure.

Biological function summary

CREBBP plays a pivotal role in regulating gene expression by serving as a coactivator that bridges transcription factors with the basal transcription machinery. CREBBP as part of large multiprotein complexes acetylates histones as well as non-histone proteins influencing chromatin accessibility and transcriptional efficiency. Its HAT activity contributes to the regulation of cell cycle differentiation and DNA repair.

Pathways

CREBBP integrates into both the p53 and Hedgehog signaling pathways modulating various cellular responses. In the p53 pathway CREBBP interacts with the tumor suppressor protein p53 enhancing p53’s ability to initiate expression of genes involved in cell cycle arrest and apoptosis. In the Hedgehog pathway CREBBP regulates transcription factors such as Gli proteins. The interplay between CREBBP and these pathways underlines its importance in maintaining cellular homeostasis and preventing oncogenesis.

Associated diseases and disorders

Mutations or dysregulation of CREBBP are associated with Rubinstein-Taybi syndrome and various cancers including acute myeloid leukemia. In Rubinstein-Taybi syndrome CREBBP mutations impair cognitive development and skeletal malformations. In cancer particularly acute myeloid leukemia alterations in CREBBP disrupt normal cell growth regulation and lead to tumorigenesis. The protein's interaction with factors like p300 another histone acetyltransferase further emphasizes its critical involvement in these pathological conditions.

Product promise

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6 product images

  • Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)

    Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
    Lane 2: CREBBP knockout HAP1 whole cell lysate (40 μg)
    Lane 3: HeLa whole cell lysate (40 μg)
    Lane 4: NIH3T3 whole cell lysate (40 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab50702 observed at 265 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 50 kDa.

    ab50702 was shown to specifically recognize CREBBP in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CREBBP knockout samples were examined. Wild-type and CREBBP knockout samples were subjected to SDS-PAGE. ab50702 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)

    Predicted band size: 265 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (ab50702)

    IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50702, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CREBBP antibody [CBP5I001] (ab50702)

    Staining of HeLa cell lysate with ab50702 at a dilution of 1/5 and Goat anti-mouse secondary at 1/200.

  • Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)

    All lanes: Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/30 dilution

    All lanes: RIPA lysate of HeLa cells at 500 µg

    Secondary

    All lanes: Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 265 kDa

  • Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)

    All lanes: Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/50 dilution

    All lanes: NIH3T3 whole cell lysate at 50 µg

    Secondary

    All lanes: Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 265 kDa

  • Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702), expandable thumbnail

    Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702)

    All lanes: Western blot - Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/100 dilution

    All lanes: HeLa whole cell lysate at 50 µg

    Secondary

    All lanes: Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 265 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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