Anti-CREG1/CREG antibody

• WB

Supplier Data

Western blot - Anti-CREG1/CREG antibody (AB191909)

All lanes:

Western blot - Anti-CREG1/CREG antibody (ab191909)

Lane 1:

HEK293T whole cell lysate

Lane 2:

RAW 264.7 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 24 kDa

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• WB

CiteAb

Western blot - Anti-CREG1/CREG antibody (AB191909)

CREG1/CREG western blot using anti-CREG1/CREG antibody ab191909. Publication image and figure legend from Liu, Y., Tian, X., et al., 2016, PLoS One, PubMed 27139506.

ab191909 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab191909 please see the product overview.

Effect of GATA1 over-expression on high glucose/high palmitate-induced apoptosis and CREG expression in HUVECs.GATA1 over-expression was achieved by transfecting HUVECs with pcDNA 3.1(+) containing full length human GATA1 cDNA. HUVECs or HUVECs transfected with empty pcDNA 3.1(+) were used as controls. Cells were treated with high glucose/high palmitate concentrations for 24 hours prior to the following analyses. (A) Apoptosis was assessed via Annexin V/PI dual-colour flow cytometry. Cells staining positive for Annexin V-FITC and negative for PI were considered to be undergoing apoptosis. (B) Apoptosis was assessed via TUNEL staining. (C) Expression of CREG and cleaved caspase-3 were determined via immunoblot. (D) Expression of CREG and cleaved caspase-3 were determined via Real-time PCR. GAPDH was used as a loading control. n = 4. **, ##p<0.01 compared with control HUVECs or HUVECs transfected with the pcDNA 3.1 (+) vehicle control.

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