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AB327171

Anti-CREM Antibody

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Rabbit Polyclonal ICER antibody. Suitable for WB, IHC-P, ChIP and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human cAMP-responsive element modulator.

View Alternative Names

cAMP-responsive element modulator, Inducible cAMP early repressor, ICER, CREM, cAMP-responsive element modulator, Inducible cAMP early repressor, ICER, cAMP-responsive element modulator

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)

Immunohistochemical staining of human testis using ab327171 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in spermatids.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)

Immunohistochemistry analysis in human testis and fallopian tube tissues using ab327171 at 1 : 500–1 : 1000 dilution. Corresponding CREM RNA-seq data are presented for the same tissues.

ChIP - Anti-CREM Antibody (AB327171)
  • ChIP

Supplier Data

ChIP - Anti-CREM Antibody (AB327171)

ChIP-Exo-Seq composite graph for ab327171 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-CREM Antibody (AB327171)
  • WB

Supplier Data

Western blot - Anti-CREM Antibody (AB327171)

Western blot analysis in human cell lines PC-3 and Caco-2 using ab327171 at 0.04–0.4 µg/ml. Corresponding CREM RNA-seq data are presented for the same cell lines. Loading control : Anti-COX4I1 antibody.

All lanes:

Western blot - Anti-CREM Antibody (ab327171)

Lane 2:

Human cell lines PC-3

Lane 3:

Human cell lines Caco-2

Predicted band size: 37.01 kDa

false

Western blot - Anti-CREM Antibody (AB327171)
  • WB

Supplier Data

Western blot - Anti-CREM Antibody (AB327171)

Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using ab327171 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-CREM Antibody (ab327171)

Lane 2:

Mouse cell line NIH-3T3

Lane 3:

Rat cell line NBT-II

Lane 4:

Rat cell line pC12

Predicted band size: 37.01 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)

Immunohistochemical staining of human fallopian tube using ab327171 at 1 : 500–1 : 1000 dilution, showing no nuclear positivity in glandular cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)

Immunohistochemical staining of human tonsil using ab327171 at 1 : 500–1 : 1000 dilution, showing no nuclear positivity in non-germinal center cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREM Antibody (AB327171)

Immunohistochemical staining of human prostate using ab327171 at 1 : 500–1 : 1000 dilution, showing no nuclear positivity in glandular cells as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ChIP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human cAMP-responsive element modulator.

Q03060

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation (PubMed : 10373550).. Isoform 6. May play a role in the regulation of the circadian clock : acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.
See full target information cAMP-responsive element modulator

Additional targets

,

Product promise

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