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AB222312

Anti-CREST antibody

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(1 Publication)

Rabbit Polyclonal CREST antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SS18L1 aa 50-150.
1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (AB222312)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (AB222312)

PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for CREST (green) using ab222312 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SS18L1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

O75177

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CREST also known as SS18L1 is a 406 amino acid protein with a molecular weight of approximately 46 kDa. It is widely expressed in the central nervous system particularly in neurons. As a member of the SWI/SNF chromatin remodeling complex CREST plays a significant role in transcriptional regulation by altering chromatin architecture. Researchers often utilize CREST antibodies in experiments to study its localization and expression levels with CREST staining being a useful technique in identifying its presence in neuronal tissues.
Biological function summary

CREST functions as a regulator of gene transcription which is essential for various neuronal activities such as dendritic development and synaptic plasticity. As a component of the SWI/SNF complex CREST interacts with other proteins to modulate the transcription of genes required for neuronal differentiation and activity-dependent responses. These interactions help to facilitate the structural and functional modifications necessary for synaptic development and neurotransmission.

Pathways

CREST interacts within the calcium signaling pathway and CREB signaling pathways. In the calcium signaling pathway CREST has a role in calcium-dependent regulation of gene expression impacting neuronal development. It operates closely with other proteins like CBP (CREB-binding protein) to regulate the transcription of genes that are important for neuronal plasticity and memory formation.

CREST is linked to Huntington's disease and autism spectrum disorders. In Huntington's disease CREST's role in gene transcription and synaptic plasticity may influence the progression of the disease particularly through interactions with huntingtin the protein mutated in this disorder. Similarly in autism spectrum disorders alterations in CREST function or expression may affect normal neuronal development and connectivity potentially involving connections with proteins such as MeCP2 which is implicated in neurodevelopmental disorders. CREST products including CREST antibodies serve as invaluable resources for investigating these connections in various biological and clinical studies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).
See full target information SS18L1

Alternative Names

CREST, KIAA0693, SS18L1, Calcium-responsive transactivator, SS18-like protein 1, SYT homolog 1
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Scientific reports 14:29659 PubMed39609464

2024

CMTM7 shapes the chronic inflammatory and immunosuppressive tumor microenvironment in hepatocellular carcinoma as an M2 macrophage biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Zhu,Hanzhi Liu,Huafeng Fu,Yu Luo,Baisheng Chen,Xiaofang Wu,Anran Sun,Fuxing Zhang,Tao Wang
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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