AB227535

Anti-CREST antibody

Name: Anti-CREST antibody (ab227535) | Abcam
Brand: Abcam Limited
SKU: AB227535
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CREST antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SS18L1.

View Alternative Names

CREST, KIAA0693, SS18L1, Calcium-responsive transactivator, SS18-like protein 1, SYT homolog 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREST antibody (AB227535)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREST antibody (AB227535)

Paraffin-embedded Huh-7 xenograft tissue stained for CREST with ab227535 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (AB227535)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (AB227535)

Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CREST (green) using ab227535 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-CREST antibody (AB227535)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-CREST antibody (ab227535) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa nuclear lysate at 20 µg

Predicted band size: 43 kDa

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Supplier Data

Western blot - Anti-CREST antibody (AB227535)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-CREST antibody (ab227535) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human SS18L1. The exact immunogen used to generate this antibody is proprietary information.

O75177

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CREST also known as SS18L1 is a 406 amino acid protein with a molecular weight of approximately 46 kDa. It is widely expressed in the central nervous system particularly in neurons. As a member of the SWI/SNF chromatin remodeling complex CREST plays a significant role in transcriptional regulation by altering chromatin architecture. Researchers often utilize CREST antibodies in experiments to study its localization and expression levels with CREST staining being a useful technique in identifying its presence in neuronal tissues.

Biological function summary

CREST functions as a regulator of gene transcription which is essential for various neuronal activities such as dendritic development and synaptic plasticity. As a component of the SWI/SNF complex CREST interacts with other proteins to modulate the transcription of genes required for neuronal differentiation and activity-dependent responses. These interactions help to facilitate the structural and functional modifications necessary for synaptic development and neurotransmission.

Pathways

CREST interacts within the calcium signaling pathway and CREB signaling pathways. In the calcium signaling pathway CREST has a role in calcium-dependent regulation of gene expression impacting neuronal development. It operates closely with other proteins like CBP (CREB-binding protein) to regulate the transcription of genes that are important for neuronal plasticity and memory formation.

CREST is linked to Huntington's disease and autism spectrum disorders. In Huntington's disease CREST's role in gene transcription and synaptic plasticity may influence the progression of the disease particularly through interactions with huntingtin the protein mutated in this disorder. Similarly in autism spectrum disorders alterations in CREST function or expression may affect normal neuronal development and connectivity potentially involving connections with proteins such as MeCP2 which is implicated in neurodevelopmental disorders. CREST products including CREST antibodies serve as invaluable resources for investigating these connections in various biological and clinical studies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).

See full target information SS18L1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9614 PubMed39511190

2024

The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangbeom Hyun,Jihye Ahn,Hyoungmin Kim,Jihyun Kim,Yong-In Kim,Hee-Sung Park,Robert G Roeder,J Eugene Lee,Jaehoon Kim

eLife 11: PubMed35551737

2022

Inositol polyphosphate multikinase physically binds to the SWI/SNF complex and modulates BRG1 occupancy in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoon Beon,Sungwook Han,Hyeokjun Yang,Seung Eun Park,Kwangbeom Hyun,Song-Yi Lee,Hyun-Woo Rhee,Jeong Kon Seo,Jaehoon Kim,Seyun Kim,Daeyoup Lee

Molecular medicine reports 25: PubMed35119079

2022

Exenatide exerts a neuroprotective effect against diabetic cognitive impairment in rats by inhibiting apoptosis: Role of the JNK/c‑JUN signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gengyin Wang,Zongquan Zhao,Bo Ren,Wu Yu,Xudong Zhang,Jiang Liu,Liping Wang,Daowen Si,Meiliu Yang

Autophagy 18:643-660 PubMed34229552

2021

RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jin,Kehan Wang,Lu Wang,Wenwen Liu,Chi Zhang,Yuexin Qiu,Wei Liu,Huiyu Zhang,Dong Zhang,Zhixia Yang,Tinghe Wu,Jing Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CREST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREST antibody (AB227535)

Immunocytochemistry/ Immunofluorescence - Anti-CREST antibody (AB227535)

Western blot - Anti-CREST antibody (AB227535)

Western blot - Anti-CREST antibody (AB227535)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise