JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB216599

Anti-CRF antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal CRF antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Corticoliberin aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF, CRH, CRH1, Corticoliberin, Corticotropin releasing factor, Corticotropin releasing hormone, Crh

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody (AB216599)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody (AB216599)

Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of rat kidney tissue labeling CRF with ab216599 at a 1/200 dilution, followed by conjugation with a goat anti-rabbit IgG PE conjugated secondary at 1/200 for 40 minutes at 37°C. DAPI (5 μg/mL, blue) was used to stain the cell nuclei.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody (AB216599)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody (AB216599)

Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of rat kidney tissue labeling CRF with ab216599 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Corticoliberin aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P06850

style
left-column

Alternative Products

Primary Antibodies

AB272391

Anti-CRF antibody [EPR23255-162]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

  • 0
  • 0
Primary Antibodies

AB231443

Anti-CRF antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody - C-terminal (AB231443)

  • 0
  • 0
Primary Antibodies

AB184238

Anti-CRF antibody [EPR19154]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

  • 5
  • 1
Primary Antibodies

AB226911

Anti-CRF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody (AB226911)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB254257

Anti-POMC antibody [EPR22534-165]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR22534-165] (AB254257)

  • 4
  • 1
Primary Antibodies

AB30914

Anti-Neuropeptide Y antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody (AB30914)

  • 5
  • 17
Primary Antibodies

AB77686

Anti-CRF1/CRHR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)

  • 0
  • 0
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>For IF please use a dilution of 1/50 - 1/200.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Corticotropin-releasing factor (CRF) also known as corticotropin-releasing hormone (CRH) is a peptide hormone involved in the stress response. This protein with a mass of approximately 41 kDa is predominantly produced in the hypothalamus and later secreted into the bloodstream. CRF plays an important role in activating the hypothalamic-pituitary-adrenal (HPA) axis stimulating the secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland. Its expression extends beyond the hypothalamus being present in peripheral tissues such as the adrenal glands and gastrointestinal tract.
Biological function summary

Corticotropin-releasing factor functions as a central coordinator of the body's response to stress. CRF engages several receptors including CRF receptors type 1 and 2 which mediate its actions. It forms part of a larger complex involving several other binding proteins that regulate its availability and receptor affinity. By interacting with these receptors CRF modulates diverse physiological processes such as immune responses and energy balance ensuring the maintenance of homeostasis under stress conditions.

Pathways

Corticotropin-releasing factor orchestrates important signaling within the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathways. CRF is pivotal in the activation of various signaling cascades that influence the production of glucocorticoids like cortisol. Within these pathways CRF interacts closely with proteins such as ACTH which acts downstream of CRF to potentiate adrenal corticosteroid synthesis. Another related cascade involves CRF in neuroendocrine signaling affecting neurotransmitter release and influencing behaviors related to stress and anxiety.

Corticotropin-releasing factor is heavily implicated in depression and anxiety disorders. Alterations in CRF expression and receptor sensitivity have been associated with the pathophysiology of these conditions. For instance abnormal CRF signaling can lead to elevated stress hormone levels contributing to mood disorders. CRF also connects to proteins like the glucocorticoid receptor influencing how the body processes stress-related hormones and impacting the development and progression of stress-related psychiatric disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).
See full target information Corticoliberin
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:343 PubMed41022714

2025

Deep brain stimulation of medial forebrain bundle modulates noradrenergic activity and feedforward inhibition in rodent model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Duan,Yixin Tong,Volker A Coenen,Máté D Döbrössy

The Journal of comparative neurology 531:1017-1031 PubMed37121600

2023

Uncovering the brain-wide pattern of synaptic input to vasopressin-expressing neurons in the paraventricular nucleus of the hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Woodson,Joseph F Bergan

The Journal of physiology 598:2355-2370 PubMed32266969

2020

Regular moderate exercise alleviates gastric oxidative damage in rats via the contribution of oxytocin receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Sevil Arabacı Tamer,Selen Üçem,Berk Büke,Muhammed Güner,Alp Giray Karaküçük,Niyazi Yiğit,Serap Şirvancı,Özge Çevik,Feriha Ercan,Berrak Ç Yeğen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com