AB184238

Anti-CRF antibody [EPR19154]

Name: Anti-CRF antibody [EPR19154] (ab184238) | Abcam
Brand: Abcam Limited
SKU: AB184238
Price: 270 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal CRF antibody. Suitable for WB and reacts with Rat, Mouse, Human, Recombinant full length protein - Human samples. Cited in 12 publications.

View Alternative Names

Gm1347, Crh, Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF

2 Images

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human CRF fragment recombinant protein contains aa20-185 with a His-Tag^®. This recombinant human fragment protein was made in-house.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (ab184238) at 1/1000 dilution

All lanes:

Human CRF fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (ab184238) at 1/5000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hypothalamus tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat hypothalamus tissue lysate at 20 µg

Lane 6:

Mouse pancreas tissue lysate at 20 µg

Lane 7:

Mouse skin tissue lysate at 20 µg

Lane 8:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 25 kDa

false

Exposure time: 3min

Carrier free

Anti-CRF antibody [EPR19154] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19154

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Corticotropin-releasing factor (CRF) also known as corticotropin-releasing hormone (CRH) is a peptide hormone involved in the stress response. This protein with a mass of approximately 41 kDa is predominantly produced in the hypothalamus and later secreted into the bloodstream. CRF plays an important role in activating the hypothalamic-pituitary-adrenal (HPA) axis stimulating the secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland. Its expression extends beyond the hypothalamus being present in peripheral tissues such as the adrenal glands and gastrointestinal tract.

Biological function summary

Corticotropin-releasing factor functions as a central coordinator of the body's response to stress. CRF engages several receptors including CRF receptors type 1 and 2 which mediate its actions. It forms part of a larger complex involving several other binding proteins that regulate its availability and receptor affinity. By interacting with these receptors CRF modulates diverse physiological processes such as immune responses and energy balance ensuring the maintenance of homeostasis under stress conditions.

Pathways

Corticotropin-releasing factor orchestrates important signaling within the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathways. CRF is pivotal in the activation of various signaling cascades that influence the production of glucocorticoids like cortisol. Within these pathways CRF interacts closely with proteins such as ACTH which acts downstream of CRF to potentiate adrenal corticosteroid synthesis. Another related cascade involves CRF in neuroendocrine signaling affecting neurotransmitter release and influencing behaviors related to stress and anxiety.

Corticotropin-releasing factor is heavily implicated in depression and anxiety disorders. Alterations in CRF expression and receptor sensitivity have been associated with the pathophysiology of these conditions. For instance abnormal CRF signaling can lead to elevated stress hormone levels contributing to mood disorders. CRF also connects to proteins like the glucocorticoid receptor influencing how the body processes stress-related hormones and impacting the development and progression of stress-related psychiatric disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (PubMed : 23470617).

See full target information Crh

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:11099-11111 PubMed40261603

2025

Plasma-Derived Small Extracellular Vesicles miR- 182 - 5p Is a Potential Biomarker for Diagnosing Major Depressive Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Zhu,Lian-Di Li,Xuan-Yu Lin,Jian Hu,Chun Wang,Yi-Jun Wang,Qi-Gang Zhou,Jing Zhang

Molecular psychiatry 30:2407-2426 PubMed39578519

2024

Prefrontal parvalbumin interneurons mediate CRHR1-dependent early-life stress-induced cognitive deficits in adolescent male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Nu Ma,Chao-Juan Yang,Chen-Chen Zhang,Ya-Xin Sun,Xing-Duo Yao,Xiao Liu,Xue-Xin Li,Hong-Li Wang,Han Wang,Ting Wang,Xiao-Dong Wang,Chen Zhang,Yun-Ai Su,Ji-Tao Li,Tian-Mei Si

American journal of translational research 16:4549-4563 PubMed39398613

2024

Jiannao pills mitigate chronic restraint stress-induced anxiety in mice through the regulation of intestinal microflora.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Pan,Lihan Zhang,Jie Liu,Yuanyuan Liang

International journal of molecular sciences 25: PubMed38892090

2024

Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Viktor Stratilov,Sofiya Potapova,Diana Safarova,Ekaterina Tyulkova,Oleg Vetrovoy

Nature communications 14:5601 PubMed37699899

2023

Solar ultraviolet B radiation promotes α-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Huang,Lin Zhu,Shipeng Cheng,Ranran Dai,Chunrong Huang,Yanyan Song,Bo Peng,Xuezhen Li,Jing Wen,Yi Gong,Yunqian Hu,Ling Qian,Linyun Zhu,Fengying Zhang,Li Yu,Chunyan Yi,Wangpeng Gu,Zhiyang Ling,Liyan Ma,Wei Tang,Li Peng,Guochao Shi,Yaguang Zhang,Bing Sun

Frontiers in molecular neuroscience 16:1115569 PubMed36760604

2023

Electroacupuncture alleviates perioperative hypothalamus-pituitary-adrenal axis dysfunction circRNA-miRNA-mRNA networks.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Wei Hu,Jing Han,Jiayuan Zheng,Ning Jiang,Yi Feng,Zhanzhuang Tian

Cells 12: PubMed36611953

2022

Sleep-Disturbance-Induced Microglial Activation Involves CRH-Mediated Galectin 3 and Autophagy Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Liyang Guo,Kirstin M Reed,Ashley Carter,Yan Cheng,Soheil Kazemi Roodsari,Damian Martinez Pineda,Laurie L Wellman,Larry D Sanford,Ming-Lei Guo

Nutrients 14: PubMed35276788

2022

Urocortin Neuropeptide Levels Are Impaired in the PBMCs of Overweight Children.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Kavalakatt,Abdelkrim Khadir,Shihab Kochumon,Dhanya Madhu,Sriraman Devarajan,Maha Hammad,Nada Alam-Eldin,Samia Warsame,Hessa Al-Kandari,Maria AlMahdi,Rasheed Ahmad,Heikki A Koistinen,Jaakko Tuomilehto,Fahd Al-Mulla,Jehad Abubaker,Ali Tiss

Frontiers in neuroscience 15:703044 PubMed34658758

2021

GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jing Han,Jing Zhu,Mizhen Zhang,Minda Ju,Yueshan Du,Zhanzhuang Tian

The ISME journal 14:2625-2645 PubMed32632263

2020

Coprophagy prevention alters microbiome, metabolism, neurochemistry, and cognitive behavior in a small mammal.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Bei Bo,Xue-Ying Zhang,Kevin D Kohl,Jing Wen,Shuang-Jie Tian,De-Hua Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRF antibody [EPR19154]

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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