Anti-CRF antibody [EPR19154] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] - BSA and Azide free (AB250741)

This data was developed using ab184238, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human CRF fragment recombinant protein contains aa20-185 with a His-Tag^®. This recombinant human fragment protein was made in-house.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (<a href='/en-us/products/primary-antibodies/crf-antibody-epr19154-ab184238'>ab184238</a>) at 1/1000 dilution

All lanes:

Human CRF fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] - BSA and Azide free (AB250741)

This data was developed using ab184238, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (<a href='/en-us/products/primary-antibodies/crf-antibody-epr19154-ab184238'>ab184238</a>) at 1/5000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hypothalamus tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat hypothalamus tissue lysate at 20 µg

Lane 6:

Mouse pancreas tissue lysate at 20 µg

Lane 7:

Mouse skin tissue lysate at 20 µg

Lane 8:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 25 kDa

false

Exposure time: 3min