AB272391

Anti-CRF antibody [EPR23255-162]

BOND RX™ Validated
RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal CRF antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF, CRH, CRH1, Corticoliberin, Corticotropin releasing factor, Corticotropin releasing hormone, Crh

4 Images

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1000 (2 μg/ml) dilution (Green). Positive staining on median eminence of rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondaryat 1000 (2 μg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus is observed (PMID : 28883467). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1000 (2 μg/ml) dilution (Green). Positive staining on median eminence of mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondaryat 1000 (2 μg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus is observed (PMID : 23724107). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Carrier free

Anti-CRF antibody [EPR23255-162] - BSA and Azide free
578 PE

PE Anti-CRF antibody [EPR23255-162]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CRF antibody [EPR23255-162]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CRF antibody [EPR23255-162]
660 APC

APC Anti-CRF antibody [EPR23255-162]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CRF antibody [EPR23255-162]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CRF antibody [EPR23255-162]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CRF antibody [EPR23255-162]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23255-162

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Corticotropin-releasing factor (CRF) also known as corticotropin-releasing hormone (CRH) is a peptide hormone involved in the stress response. This protein with a mass of approximately 41 kDa is predominantly produced in the hypothalamus and later secreted into the bloodstream. CRF plays an important role in activating the hypothalamic-pituitary-adrenal (HPA) axis stimulating the secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland. Its expression extends beyond the hypothalamus being present in peripheral tissues such as the adrenal glands and gastrointestinal tract.

Biological function summary

Corticotropin-releasing factor functions as a central coordinator of the body's response to stress. CRF engages several receptors including CRF receptors type 1 and 2 which mediate its actions. It forms part of a larger complex involving several other binding proteins that regulate its availability and receptor affinity. By interacting with these receptors CRF modulates diverse physiological processes such as immune responses and energy balance ensuring the maintenance of homeostasis under stress conditions.

Pathways

Corticotropin-releasing factor orchestrates important signaling within the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathways. CRF is pivotal in the activation of various signaling cascades that influence the production of glucocorticoids like cortisol. Within these pathways CRF interacts closely with proteins such as ACTH which acts downstream of CRF to potentiate adrenal corticosteroid synthesis. Another related cascade involves CRF in neuroendocrine signaling affecting neurotransmitter release and influencing behaviors related to stress and anxiety.

Corticotropin-releasing factor is heavily implicated in depression and anxiety disorders. Alterations in CRF expression and receptor sensitivity have been associated with the pathophysiology of these conditions. For instance abnormal CRF signaling can lead to elevated stress hormone levels contributing to mood disorders. CRF also connects to proteins like the glucocorticoid receptor influencing how the body processes stress-related hormones and impacting the development and progression of stress-related psychiatric disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

See full target information Corticoliberin

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry : PubMed41053436

2025

Regulation of stress susceptibility by chromatin-binding protein PHF6 in the pituitary intermediate lobe.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Jingjie Wang,Xiaohua Wu,Linhua Gan,Jingjing Sun,Guangzhong Wang,Qian Li,Ju Huang

Nature communications 16:8870 PubMed41053178

2025

Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Tao Liu,Yang Wang,Xue-Mei Miao,Yi-Yu Xiong,Qian Zhao,Wei-Yun Shen,Fu-Hong Su,Kang Chen,Ru-Ping Dai

World journal of psychiatry 15:107342 PubMed40837817

2025

Electroacupuncture ameliorates visceral hypersensitivity and negative emotions by regulating paraventricular hypothalamic nucleus and colonic corticotropin-releasing factor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Gang Xu,Yi Yuan,Hong-Kun Ma,Shun Huang,Shan-Lan Zhu,Tan-Ting Li,Xi-Yang Wang,Guo-Ming Shen,Hao Wang

Endocrinology 166: PubMed40302251

2025

Serotonergic and Chemosensory Brain Areas and Sensory Ganglia Expressing Type 3 Deiodinase Mapped With Dio3Cre drivers.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Liu,Lily Ng,Chengyu Liu,Douglas Forrest

eLife 12: PubMed39526880

2024

Hypothalamic corticotropin-releasing hormone neurons modulate sevoflurane anesthesia and the post-anesthesia stress responses.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Jiang,Lu Chen,Wei-Min Qu,Zhi-Li Huang,Chang-Rui Chen

EMBO reports 24:e57176 PubMed37870400

2023

Role and mechanism of PVN-sympathetic-adipose circuit in depression and insulin resistance induced by chronic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Linshan Sun,Jingjing You,Honghai Peng,Haijing Yan,Jiangong Wang,Fengjiao Sun,Minghu Cui,Sanwang Wang,Zheng Zhang,Xueli Fan,Dunjiang Liu,Cuilan Liu,Changyun Qiu,Chao Chen,Zhicheng Xu,Jinbo Chen,Wei Li,Bin Liu

Nature communications 14:5601 PubMed37699899

2023

Solar ultraviolet B radiation promotes α-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Huang,Lin Zhu,Shipeng Cheng,Ranran Dai,Chunrong Huang,Yanyan Song,Bo Peng,Xuezhen Li,Jing Wen,Yi Gong,Yunqian Hu,Ling Qian,Linyun Zhu,Fengying Zhang,Li Yu,Chunyan Yi,Wangpeng Gu,Zhiyang Ling,Liyan Ma,Wei Tang,Li Peng,Guochao Shi,Yaguang Zhang,Bing Sun

Cancer research communications 3:1524-1537 PubMed37575281

2023

Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline L E Tearle,Satya N V Arjunan,Szun S Tay,Feyza Colakoglu,James Cremasco,Matteo Golo,Maté Biro

The Journal of physiology 601:2853-2875 PubMed37154244

2023

Perinatal high-fat diet exposure alters oxytocin and corticotropin releasing factor inputs onto vagal neurocircuits controlling gastric motility.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlin E Carson,Jared Alvarez,Jasmine Q Mackley,R Alberto Travagli,Kirsteen N Browning

The Journal of biological chemistry 299:102728 PubMed36410433

2022

rAAV-CRISPRa therapy corrects Rai1 haploinsufficiency and rescues selective disease features in Smith-Magenis syndrome mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Cheng Chang,Yu-Ju Lee,Sehrish Javed,Minza Haque,Ya-Ting Chang,Yu Cheng Lin,Cameron Oram,Wei-Hsiang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRF antibody [EPR23255-162]

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise