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AB77686

Anti-CRF1/CRHR1 antibody

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(10 Publications)

Goat Polyclonal CRF1/CRHR1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human CRHR1 aa 100-150.

View Alternative Names

CRFR, CRFR1, CRHR, CRHR1, Corticotropin-releasing factor receptor 1, CRF-R-1, CRF-R1, CRFR-1, Corticotropin-releasing hormone receptor 1, CRH-R-1, CRH-R1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)

Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling CRF1 with ab77686 at 10μg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody was used as the secondary antibody (green) showing cytoplasmic, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control : Unimmunized goat IgG (10μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/mL).

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)

Immunocytochemistry/immunofluorescence analysis of Neuro2a cells labelling CRF1 with ab77686 at 10μg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody at 2ug/ml was used as the secondary antibody (green) showing plasma membrane, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (2ug/ml).

Western blot - Anti-CRF1/CRHR1 antibody (AB77686)
  • WB

Supplier Data

Western blot - Anti-CRF1/CRHR1 antibody (AB77686)

Incubated with the primary antibody for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CRF1/CRHR1 antibody (ab77686) at 1 µg/mL

Lane 1:

Human colon tissue lysate in RIPA buffer at 35 µg

Lane 2:

Human cerebellum tissue lysate in RIPA buffer at 35 µg

Lane 3:

Rat brain tissue lysate in RIPA buffer at 35 µg

Lane 4:

Mouse spinal cord tissue lysate in RIPA buffer at 35 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CRHR1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P34998

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.3 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.3 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab77686 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRF1 also known as CRHR1 is a receptor for corticotropin-releasing factor (CRF) a peptide hormone involved in stress response. This receptor is a G protein-coupled receptor with a mass of approximately 50 kDa. CRHR1 is expressed in the central nervous system especially in the brain regions like the cortex and the cerebellum. It can also be found in peripheral tissues like the pituitary gland. Its localization is important because it is involved in mediating the effects of CRF across various tissues.
Biological function summary

CRHR1 plays a significant role in stress-related responses and is involved in the modulation of the hypothalamic-pituitary-adrenal (HPA) axis. When CRF binds to this receptor it triggers the release of adrenocorticotropic hormone (ACTH) from the pituitary gland. CRHR1 is also part of the CRF receptor family complex which includes CRHR2 another receptor with similar functions. The interaction with CRF can initiate various signaling pathways further affecting physiological responses to stress.

Pathways

CRHR1 integrates into the HPA axis pathway as well as the cyclic AMP (cAMP) signaling pathway. In the HPA pathway CRHR1's activation leads to the secretion of ACTH ultimately resulting in cortisol production by the adrenal glands. The cAMP-responsive element-binding protein (CREB) may become activated in response to CRHR1 modulation influencing gene expression and cellular response to stress. The CRHR1 receptor therefore plays a critical role in these pathways by linking stress signals to hormonal and behavioral changes.

CRHR1 has a strong connection to stress-related conditions such as depression and anxiety. Dysregulation in CRHR1 signaling can lead to altered HPA axis functioning which contributes to the development of these disorders. Stress-induced activation of CRHR1 is associated with increased levels of cortisol which is commonly seen in individuals with depression. Furthermore CRHR1 interacts closely with other proteins like the glucocorticoid receptor (GR) which also play a part in these psychiatric conditions indicating a complex network of interactions that govern stress responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
See full target information CRHR1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009640

2025

CD163/CD63+ Monocyte-Derived DC Profiled in Tissue by Multi-Antigen Analysis (MAA) Discriminate Chronic Eczema and Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Windorfer,Michael Kirr,Waltraud Fröhlich,Bianca Plosnita,Christian Ostalecki,Carola Berking,Michael Sticherling,Andreas S Baur

Science advances 10:eadk7636 PubMed38728397

2024

Hyperexcitation of ovBNST CRF neurons during stress contributes to female-biased expression of anxiety-like avoidance behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Na Zhang,Sha Zhao,Yanqiao Ma,Zhixin Xiao,Bao Xue,Yuan Dong,Qingyu Wang,Huamin Xu,Xia Zhang,Ying Wang

iScience 27:109675 PubMed38706837

2024

extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhyoung Lee,So Young Kim,Seunghee Lee,Sunhyae Jang,Sungjoo Tommy Hwang,Youngji Kwon,Jaehwan Choi,Ohsang Kwon

The international journal of neuropsychopharmacology 26:828-839 PubMed37864842

2023

The Impact of Heroin Self-Administration and Environmental Enrichment on Ventral Tegmental CRF1 Receptor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Galaj,Eddy D Barrera,Kirk Persaud,Rudolf Nisanov,Apoorva Vashisht,Hindy Goldberg,Nima Patel,Hayley Lenhard,Zhi-Bing You,Eliot L Gardner,Robert Ranaldi

International journal of molecular sciences 23: PubMed35955675

2022

Psychological Stress Exacerbates Inflammation of the Ileum via the Corticotropin-Releasing Hormone-Mast Cell Axis in a Mouse Model of Eosinophilic Enteritis.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Kanamori,Fumio Tanaka,Masaki Ominami,Yuji Nadatani,Shusei Fukunaga,Koji Otani,Shuhei Hosomi,Noriko Kamata,Yasuaki Nagami,Koichi Taira,Yasuhiro Fujiwara

Reproduction (Cambridge, England) 162:11-20 PubMed33929340

2021

Urocortins exhibit differential effects on PGE2 and PGF2α output via CRHR2 in human myometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Xingji You,Zixi Chen,Qianqian Sun,Ruojin Yao,Hang Gu,Xin Ni

International journal of molecular sciences 22: PubMed33803422

2021

Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Yamanaka-Takaichi,Yukari Mizukami,Koji Sugawara,Kishiko Sunami,Yuichi Teranishi,Yukimi Kira,Ralf Paus,Daisuke Tsuruta

Journal of leukocyte biology 102:1299-1312 PubMed28684600

2017

Frontline Science: Corticotropin-releasing factor receptor subtype 1 is a critical modulator of mast cell degranulation and stress-induced pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Saravanan Ayyadurai,Amelia J Gibson,Susan D'Costa,Elizabeth L Overman,Laura J Sommerville,Ashwini C Poopal,Emily Mackey,Yihang Li,Adam J Moeser

Molecular and cellular endocrinology 437:312-322 PubMed27524410

2016

Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna M Wierzbicka,Michał A Żmijewski,Anna Piotrowska,Boguslaw Nedoszytko,Magdalena Lange,Robert C Tuckey,Andrzej T Slominski

Journal of cellular physiology 232:154-66 PubMed27061711

2016

Differentiation of Keratinocytes Modulates Skin HPA Analog.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna M Wierzbicka,Michał A Żmijewski,Jakub Antoniewicz,Michal Sobjanek,Andrzej T Slominski
View all publications
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