JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236982

Anti-CRHR2 antibody

0

(1 Review)

|

(3 Publications)

Rabbit Polyclonal CRHR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CRHR2 aa 1-150.

View Alternative Names

CRF2R, CRH2R, CRHR2, Corticotropin-releasing factor receptor 2, CRF-R-2, CRF-R2, CRFR-2, Corticotropin-releasing hormone receptor 2, CRH-R-2, CRH-R2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRHR2 antibody (AB236982)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRHR2 antibody (AB236982)

Paraffin-embedded human adrenal gland tissue stained for CRHR2 with ab236982 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CRHR2 antibody (AB236982)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRHR2 antibody (AB236982)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CRHR2 using ab236982 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-CRHR2 antibody (AB236982)
  • WB

Supplier Data

Western blot - Anti-CRHR2 antibody (AB236982)

All lanes:

Western blot - Anti-CRHR2 antibody (ab236982) at 3 µg/mL

Lane 1:

Rat brain tissue

Lane 2:

Mouse brain tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CRHR2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q13324

style
left-column

Alternative Products

Primary Antibodies

AB150510

Anti-CRHR2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRHR2 antibody (AB150510)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB77686

Anti-CRF1/CRHR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB272391

Anti-CRF antibody [EPR23255-162]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (AB272391)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Corticotropin-releasing hormone receptor 2 (CRHR2) also known as CRF2 receptor is a protein encoded by the CRHR2 gene. Mechanically CRHR2 functions by binding to corticotropin-releasing hormone (CRH) and related peptides leading to activation of intracellular signaling pathways. This receptor is a member of the G protein-coupled receptor family and is approximately 47 kDa in mass. CRHR2 is expressed in diverse tissues prominently in the brain heart gastrointestinal tract and skeletal muscle.
Biological function summary

The receptor regulates stress-related and homeostatic functions. It plays a significant role in mediating the effects of CRH and urocortins on the central nervous system and peripheral tissues. The receptor contributes to managing anxiety cardiovascular function gastric motility and energy homeostasis. Although CRHR2 does not form a part of a larger complex it closely interacts with other neuropeptides and their receptors to maintain physiological balance.

Pathways

CRHR2 is integrated into important signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathway. In these pathways CRHR2 influences cortisol release and modulates stress responses. Related proteins include CRHR1 which also binds CRH facilitating modulation of adaptive responses to stress. Additionally CRHR2 interacts with intracellular proteins that regulate signal transduction cascades linked to mood regulation and anxiety management.

CRHR2 has associations with conditions like depression and cardiovascular diseases. Elevated or altered activity of CRHR2 can affect mood regulation pathways leading to an increased risk of depression. The receptor's role in cardiovascular regulation links it to heart diseases where altered signaling can impact heart rate and blood pressure. CRHR2 is associated with proteins such as CRH which can jointly influence the susceptibility to these disorders by modulating stress and cardiovascular responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
See full target information CRHR2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109675 PubMed38706837

2024

extract attenuates corticotropin-releasing hormone-induced cellular senescence in human hair follicle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhyoung Lee,So Young Kim,Seunghee Lee,Sunhyae Jang,Sungjoo Tommy Hwang,Youngji Kwon,Jaehwan Choi,Ohsang Kwon

The Journal of physiology 602:3375-3400 PubMed38698722

2024

Oxytocin and corticotropin-releasing hormone exaggerate nucleus tractus solitarii neuronal and synaptic activity following chronic intermittent hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Procopio Gama de Barcellos Filho,Heather A Dantzler,Eileen M Hasser,David D Kline

Cell reports 31:107681 PubMed32460023

2020

Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Stiff,Fabio R Echegaray-Iturra,Harry J Pink,Alex Herbert,Constantino Carlos Reyes-Aldasoro,Helfrid Hochegger
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com