Rabbit Polyclonal CRHR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRHR2 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CRHR2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13324
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 3 µg/mL | Notes - |
Species Rat | Dilution info 3 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
Alternative names
CRF2R, CRH2R, CRHR2, Corticotropin-releasing factor receptor 2, CRF-R-2, CRF-R2, CRFR-2, Corticotropin-releasing hormone receptor 2, CRH-R-2, CRH-R2
Recommended products
Rabbit Polyclonal CRHR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CRHR2 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CRHR2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13324
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Corticotropin-releasing hormone receptor 2 (CRHR2) also known as CRF2 receptor is a protein encoded by the CRHR2 gene. Mechanically CRHR2 functions by binding to corticotropin-releasing hormone (CRH) and related peptides leading to activation of intracellular signaling pathways. This receptor is a member of the G protein-coupled receptor family and is approximately 47 kDa in mass. CRHR2 is expressed in diverse tissues prominently in the brain heart gastrointestinal tract and skeletal muscle.
- Biological function summary
The receptor regulates stress-related and homeostatic functions. It plays a significant role in mediating the effects of CRH and urocortins on the central nervous system and peripheral tissues. The receptor contributes to managing anxiety cardiovascular function gastric motility and energy homeostasis. Although CRHR2 does not form a part of a larger complex it closely interacts with other neuropeptides and their receptors to maintain physiological balance.
- Pathways
CRHR2 is integrated into important signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathway. In these pathways CRHR2 influences cortisol release and modulates stress responses. Related proteins include CRHR1 which also binds CRH facilitating modulation of adaptive responses to stress. Additionally CRHR2 interacts with intracellular proteins that regulate signal transduction cascades linked to mood regulation and anxiety management.
- Associated diseases and disorders
CRHR2 has associations with conditions like depression and cardiovascular diseases. Elevated or altered activity of CRHR2 can affect mood regulation pathways leading to an increased risk of depression. The receptor's role in cardiovascular regulation links it to heart diseases where altered signaling can impact heart rate and blood pressure. CRHR2 is associated with proteins such as CRH which can jointly influence the susceptibility to these disorders by modulating stress and cardiovascular responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRHR2 antibody (ab236982)
Paraffin-embedded human adrenal gland tissue stained for CRHR2 with ab236982 at a 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-CRHR2 antibody (ab236982)
All lanes: Western blot - Anti-CRHR2 antibody (ab236982) at 3 µg/mL
Lane 1: Rat brain tissue
Lane 2: Mouse brain tissue
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CRHR2 antibody (ab236982)
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CRHR2 using ab236982 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
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