AB226244

Anti-CRIF1 antibody

Name: Anti-CRIF1 antibody (ab226244) | Abcam
Brand: Abcam Limited
SKU: AB226244
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal CRIF1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GADD45GIP1 aa 150-200.

View Alternative Names

MRPL59, PLINP1, PRG6, GADD45GIP1, Large ribosomal subunit protein mL64, CKII beta-associating protein, CR6-interacting factor 1, Growth arrest and DNA damage-inducible proteins-interacting protein 1, Papillomavirus L2-interacting nuclear protein 1, p53-responsive gene 6 protein, MRP-L59, CRIF1, PLINP, PLINP-1

2 Images

Supplier Data

Immunoprecipitation - Anti-CRIF1 antibody (AB226244)

CRIF1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226244 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226244 at 1 μg/ml.

Lane 1 : ab226244 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CRIF1 antibody (ab226244)

Predicted band size: 25 kDa

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Supplier Data

Western blot - Anti-CRIF1 antibody (AB226244)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CRIF1 antibody (ab226244) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 25 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GADD45GIP1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q8TAE8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226244 was affinity purified using an epitope specific to CRIF1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRIF1 also known as CRIFone is a protein implicated in coordinating mitochondrial translation and metabolism. It has a molecular weight of approximately 33 kDa. The gene encoding CRIF1 is found in humans and is expressed in various tissues including liver heart and skeletal muscle indicating its significant role in energy-demanding tissues. CRIF1 localizes mainly in the mitochondrial inner membrane where it interacts with mitoribosomes to facilitate the synthesis and integration of respiratory chain complexes.

Biological function summary

CRIF1 functionally contributes to the mitochondrial protein synthesis machinery and is a part of the mitoribosome complex. It directly interacts with nascent polypeptide chains during mitochondrial translation ensuring proper insertion into the mitochondrial inner membrane. This interaction is essential for effective cellular respiration and ATP production. The protein further plays a role in maintaining mitochondrial structure and regulating cellular energy homeostasis by stabilizing key mitochondrial complexes particularly involved in oxidative phosphorylation.

Pathways

CRIF1 participates noticeably in the oxidative phosphorylation and mitochondrial biogenesis pathways. In these pathways CRIF1 ensures the proper assembly and maintenance of the electron transport chain complexes. It interacts with other mitochondrial proteins such as COX1 and ND1 integral parts of Complex IV and Complex I of the electron transport chain respectively. These interactions position CRIF1 as critical for efficient energy production and metabolic regulation.

CRIF1 has been linked to metabolic syndromes and neurodegenerative diseases. Its dysregulation can impact mitochondrial function leading to energy production deficits seen in disorders like mitochondrial myopathies and Parkinson's disease. CRIF1 interacts with proteins like Parkin known to be involved in mitochondrial quality control processes. Disruptions in CRIF1 function can exacerbate defects in mitochondrial maintenance and turnover contributing to disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

See full target information GADD45GIP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:5567-5580 PubMed38517371

2024

CRIF1 attenuates doxorubicin-mediated mitochondrial dysfunction and myocardial senescence via regulating PXDN.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zhou,Guilan Zhai,Ge Tian

iScience 26:108011 PubMed37841592

2023

A Haspin-ARHGAP11A axis regulates epithelial morphogenesis through Rho-ROCK dependent modulation of LIMK1-Cofilin.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Quadri,Giuseppe Rotondo,Sarah Sertic,Sara Pozzi,Maria Chiara dell'Oca,Luisa Guerrini,Marco Muzi-Falconi

Immunity 54:53-67.e7 PubMed33058782

2020

Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Henrique G Colaço,André Barros,Ana Neves-Costa,Elsa Seixas,Dora Pedroso,Tiago Velho,Katharina L Willmann,Pedro Faisca,Gerlinde Grabmann,Hyon-Seung Yi,Minho Shong,Vladimir Benes,Sebastian Weis,Thomas Köcher,Luís F Moita

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CRIF1 antibody

Immunoprecipitation - Anti-CRIF1 antibody (AB226244)

Western blot - Anti-CRIF1 antibody (AB226244)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

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Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise